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Diverse endophytic nitrogen-fixing bacteria isolated from wild rice Oryza rufipogon and description of Phytobacter diazotrophicus gen. nov. sp. nov.

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Abstract

Twenty-three nitrogen-fixing bacteria were isolated from surface-sterilized stems and roots of wild rice Oryza rufipogon. Four clusters were defined among these bacteria by SDS-PAGE protein patterns and further confirmed by IS-PCR finger-printing analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the representative strains LS 8 and LS 18 of cluster II formed a monophyletic group sharing 94.0–97.3% similarities with defined enterobacterial species within the genera Salmonella, Citrobacter, Pantoea, Klebsiella, and Enterobacter. DNA–DNA hybridization, physiological, biochemical tests, and cell morphology also revealed that these strains could be differentiated from the related enterobacterial species. Based upon these results, we propose Phytobacter diazotrophicus gen. nov., sp. nov. to the bacterial group represented by strains LS 8 and LS 18. The type strain is LS 8T (=DSM 17806T = LMG 23328T = CGMCC 1.5339T). The DNA G+C content of strain LS 8T is 58.6 ± 0.5 mol%.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (30470002) and by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. We thank Dr. Jinquan Li and Dr. Xuelin Fu for providing Oryza rufipogon plants and Dr. J. Euzéby for checking the Latin name.

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Authors and Affiliations

  1. Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Guo Xia Zhang, Hui Yan, Qing Hua Yuan, Wu Zhang, Xu Lou, Hui Wu & Zhi Yuan Tan

  2. College of Resources and Environment, South China Agricultural University, Guangzhou, 510642, China

    Gui Xiang Peng

  3. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico, D. F., 11340, Mexico

    En Tao Wang

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  1. Guo Xia Zhang
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Correspondence to Zhi Yuan Tan.

Additional information

Communicated by Ercko Stackebrandt.

GenBank accession numbers: The 16S rRNA gene sequences acquired in this study have been deposited in GenBank database under the accession numbers of DQ821583, and DQ821584 respectively for strains LS 8 T and LS 18.

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Zhang, G.X., Peng, G.X., Wang, E.T. et al. Diverse endophytic nitrogen-fixing bacteria isolated from wild rice Oryza rufipogon and description of Phytobacter diazotrophicus gen. nov. sp. nov.. Arch Microbiol 189, 431–439 (2008). https://doi.org/10.1007/s00203-007-0333-7

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  • DOI: https://doi.org/10.1007/s00203-007-0333-7

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