Abstract
The objective of this research was to define an in vitro regeneration and transformation system for bell pepper (Capsicum annuum L.) using six cultivars and one Guatemalan wild accession. The wild accession exhibited the best regeneration response. Only occasional elongation of shoot buds in ‘Yolo Wonder L’ was achieved by culture in the dark on a medium containing 10 mg/l BA and l mg/l IAA. Transformed shoot buds and leaf-like structures were obtained, showing beta- glucuronidase activity predominantly in the vascular and perivascular tissues, with no indication of contaminating Agrobacterium in the tissues. Attempts to regenerate whole transgenic plants from transformed shoot buds were unsuccessful.
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Literature Cited
Aerts, M., M. Jacobs, J.-P. Hernalsteens, M. Van Montagu and J. Schell (1979). Plant Sci. Lett. 17:43–5.
Caplan, A., L. Herrera-Estrella, D. Inze, E. Van Haute, M. Van Montagu, J. Schell and P. Zambryski (1983). Sci. 222:815–821.
De Cleene, M. and J. De Lay (1976). Bot. Rev. 42:389–466.
Diaz, I., R. Moreno and J. B. Power (1988). Plant Cell Rep. 7:210–212.
Dumas de Vaulx, R., D., Chambonnet and M. Sibi (1982). In:Variability in plants regenerated from tissue culture, E. D. Earle and Y. Demarfy (eds.), Praeger Publishers, New York, pp 92–98.
Fari, M. and M. Czako (1981). Scientia Hort. 15:207–213.
Hood, E. E., G. L. Helmer, R. T. Fraley and M.-D. Chilton (1986). Bacteriol. 168:1291–1301.
Horsch, R. B., J. E. Fry, N. L. Hoffmann, D. Eichholtz, S. G. Rogers and R. T. Fraley (1985). Sci. 227:1229–1231.
Jefferson, R, A. (1987). Plant Mol. Biol. Reptr. 5:387–405.
Jefferson, R. A., T. A. Kavanagh and M. W. Bevan (1987). EMBO J. 6:3901–3907.
Jin S., T. Komari, M. P. Gordon and E. W. Nester (1987). J. Bacteriol. 169:4417–4425.
Maniatis, T., E. F. Fritsch and J. Sambrook (eds.) (1982). Cold Spring Harbor Laboratory, New York.
McCormick, S., J. Niedermeyer, J. Fry, A. Barnason, R Horsch and R. Fraley (1986). Plant Cell Pep. 5:81–84.
Murashige, T. and F. Skoog (1962). Physiol. Plant. 15:473–497.
Otten, L. A. B. M. and R. A. Schilperoort (1978). Biochim. Biophys. Acta 527:497–500.
Phillips, G. C. and G. B. Collins (1979). Crop Sci. 19:59–64.
Phillips, G. C. and J. F. Hubstenberger (1985). Plant Cell Tiss. Org. Cult. 4:261–269.
Saxena, P. K., R. Gill, A. Rashid and S. C. Maheshwari (1981). Protoplasma 108:357–360.
Schardl, C. L., A. D. Byrd, G. Benzion, M. A. Altschuler, D. F. Hildebrand and A. G. Hunt (1987). Gene 61:1–11.
Sibi, M., R., Dumas de Vaulx and D. Chambonnet (1979). Ann. Amelior. Plantes 29:583–606.
Tamm, B. (1954). Arch. Mikrobiol. 20:273–292.
Yang, N. S., B. Cohen and P. Christou (1989). Abstracts UCLA Symposium on Plant Gene Transfer, April 1–7, 1989, Park City, Utah.
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Communicated by G. C. Phillips
This paper (No. 90381) is published with the approval of the Director, Kentucky Agricultural Experiment Station, University of Kentucky, Lexington, Kentucky
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Liu, W., Parrott, W.A., Hildebrand, D.F. et al. Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot-like structures expressing introduced genes. Plant Cell Reports 9, 360–364 (1990). https://doi.org/10.1007/BF00232399
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DOI: https://doi.org/10.1007/BF00232399