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Cytotoxity of the trans10,cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids, tocopherol, and tocotrienol

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Summary

This study was performed to evaluate the isomer-specific cytotoxic effects of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. A 10trans,12cis (10t,12c)-CLA showed a strong cytotoxic effect on dRLh-84 cells in culture, whereas no such effect was observed with 9cis,11trans (9c,11t)-CLA or linoleic acid. The optimum concentration for induction of cytotoxity by 10t,12c-CLA was 5 to 10 μM, but the effect was alleviated at higher concentrations. Coincubation with oleic or palmitoleic acid and 10t,12c-CLA cancelled the cytotoxic effect, but other major saturated or polyunsaturated fatty acids and eraidic acid did not interfere with 10t,12c-CLA-induced cytotoxity. The cytotoxic effect was also alleviated by α-tocopherol (α-toc) and α-tocotrienol but not by any other antioxidant regent examined. Significant cytotoxity of 10t,12c-CLA was detected after only a 15-min incubation, and the most noticeable effect was seen after 3 h. After incubation with 10t,12c-CLA at 10 μM, an additional 90 μM, an additional 90 μM of 10t,12c-CLA or 100 μM of α-toc was also able to alleviate the cytotoxity. When cells were treated with 10 μM 10t,12c-CLA for more than 48 h, treatment with additional CLA or α-toc could not prevent cell death.

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Authors and Affiliations

  1. Department of Biochemistry and Applied BioScience, Faculty of Agriculture, Miyazaki University, 1-1 Gakuenkibanadai-nishi, 889-2192, Miyazaki, Japan

    Masao Yamasaki, Hrofumi Tachibana & Koji Yamada

  2. Laboratory of Food Chemistry, Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan

    Shinsuke Nou & Koji Yamada

  3. R&D Division, Omu Milk Products Co., Ltd, 1-38-1 Shin Katsudachi Omuta, 836-0895, Fukuoka, Japan

    Masao Yamasaki, Shinsuke Nou & Hrofumi Tachibana

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  1. Masao Yamasaki
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  2. Shinsuke Nou
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  3. Hrofumi Tachibana
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  4. Koji Yamada
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Correspondence to Masao Yamasaki.

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Yamasaki, M., Nou, S., Tachibana, H. et al. Cytotoxity of the trans10,cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids, tocopherol, and tocotrienol. In Vitro Cell.Dev.Biol.-Animal 41, 239–244 (2005). https://doi.org/10.1290/0402008.1

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  • DOI: https://doi.org/10.1290/0402008.1

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