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Differential Production of Chemokines by Phagocytosing Rat Neutrophils and Macrophages

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Abstract

In this study, rat neutrophils and macrophages produced cytokine-induced neutrophil chemoattractants (CINCs) and rat macrophage inflammatory protein-1α (MIP-lα) in different patterns during phagocytosis of heat-killed yeast cells in vitro. The cultured supernatants of the phagocytosing rat neutrophils and macrophages had chemotactic activities toward neutrophils, and the chemotactic potencies were markedly inhibited by anti-CINCs IgGs or/and anti-MIP-lα IgG, suggesting that CINCs and MIP-lα are major neutrophil chemoattractants produced by the phagocytosing neutrophils and macrophages. Dexamethasone suppressed the production of CINCs and MIP-1α by the phagocytosing cells in a dose-dependent manner. Our results demonstrate significant differences in the production of CINCs and MIP-1α by neutrophils and macrophages during phagocytosis of yeast cells and thus may suggest the different contribution of each chemokine to neutrophil recruitment in the processes of inflammation in rats.

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Authors and Affiliations

  1. Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, 930-01, Japan

    Maher Al-Mokdad, Futoshi Shibata, Katsuhiko Takano & Hideo Nakagawa

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  1. Maher Al-Mokdad
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  2. Futoshi Shibata
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  3. Katsuhiko Takano
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  4. Hideo Nakagawa
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Al-Mokdad, M., Shibata, F., Takano, K. et al. Differential Production of Chemokines by Phagocytosing Rat Neutrophils and Macrophages. Inflammation 22, 145–159 (1998). https://doi.org/10.1023/A:1022383922039

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