Abstract
Expression in Nicotiana tabaccum L. plants containing the β-glucuronidase (GUS) gene under the control of the 35S (CaMV promoter) was affected by tissue type and ontogenic development of the leaves. GUS activity in ontogenetically younger leaves was 1003–1022 nmol 7-hydroxy-4-methylcoumarin (MU) formed mg−1 (protein) min−1 and in ontogenetically older leaves was only 140–198 nmol (MU) mg−1 (protein) min−1.
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Pret'ová, A., Obert, B. & Wetzstein, H. Leaf developmental stage and tissue location affect the detection of β-glucuronidase in transgenic tobacco plants. Biotechnology Letters 23, 555–558 (2001). https://doi.org/10.1023/A:1010311524048
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DOI: https://doi.org/10.1023/A:1010311524048