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Does sorghum phenolic extract have antifungal effect?

  • Soil and Agricultural Microbiology - Research Paper
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Abstract

This study aimed to evaluate the antifungal effect of SC319 sorghum phenolic extract (SPE) on the Aspergillus, Fusarium, Penicillium, Stenocarpella, Colletotrichum, and Macrophomina genera. SPE was extracted by 20% ethanol and used in four assays: (1) against Fusarium verticillioides in solid (PDA) and liquid (PD) potato dextrose media; (2) Minimum Inhibitory Concentration (MIC) assay with 16 fungi isolates; (3) Conidial Germination Rate (CGR) with 14 fungi isolates and (4) Growth Curve (GC) with 11 fungi isolates. There was no reduction in the mycelial growth (colony diameter and dry weight) and in the number of Fusarium verticillioides spores in assay 1 (PDA and PD). The colony's dry weight was almost six times higher in the presence than in the absence of SPE. All SPE samples presented MIC (assay 1) above the maximum concentration tested (5000 µg.mL−1) for the 16 isolates. Also, there was no inhibitory effect of SPE on conidia germination rate (CGR). Oppositely, in GC assay, the control had a higher CFU count than the samples with SPE in 24 h. This result suggests that SPE can delay the fungal growth in the first hours of incubation, which is an important finding that may help reduce the severity of fungal diseases in plants. However, further studies are needed to confirm these results, including sorghum genotypes with different profiles of phenolic compounds. Although the SC319 SPE was not effective as an antifungal agent, it may have potential as a growth promoter of beneficial fungi in the food and pharmaceutical industries.

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Acknowledgements

The authors acknowledge the Empresa Brasileira de Pesquisa Agropecuária – Embrapa, Brazil (grant number SEG 20.18.03.006.00.00) and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais – FAPEMIG, Brazil (grant number APQ-00657-18) for the financial support and the Conselho Nacional de Desenvolvimento Científico e Tecnológico– CNPq, Brazil, for for the granting of the PhD student scholarship (grant number 142352/2016-0).

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Authors and Affiliations

  1. Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, no 6.627, Belo Horizonte, MG, 31270-901, Brazil

    Renata Regina Pereira da Conceição, Paulo Henrique Fonseca do Carmo & Maria Aparecida de Resende Stoianoff

  2. Embrapa Milho e Sorgo, Rodovia MG-424, Km 65, Sete Lagoas, MG, 35702-098, Brazil

    Valéria Aparecida Vieira Queiroz, Maria Lúcia Ferreira Simeone, Dagma Dionísia da Silva Araújo, Cícero Beserra de Menezes & José Edson Fontes Figueiredo

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  1. Renata Regina Pereira da Conceição
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Contributions

Renata R. P. da Conceição: Conceptualization, Methodology, Investigation, Data curation, Resources, Writing – original draft. Valéria A. V. Queiroz: Conceptualization, Methodology, Resources, Writing – review & editing, Supervision. Maria Lúcia F. Simeone: Conceptualization, Methodology, Writing – review & editing, Supervision. Dagma D. da Silva: Methodology, Writing – review & editing, Project administration, funding acquisition. Maria Aparecida de R. Stoianoff: Conceptualization, Methodology, Writing – review & editing, Supervision, and José Edson F. Figueiredo: Writing – review & editing.

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Correspondence to Valéria Aparecida Vieira Queiroz.

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da Conceição, R.R.P., Queiroz, V.A.V., Simeone, M.L.F. et al. Does sorghum phenolic extract have antifungal effect?. Braz J Microbiol 55, 1829–1839 (2024). https://doi.org/10.1007/s42770-024-01327-9

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