Abstract
The aim of this work was to give characteristic stable carbon and nitrogen isotope ratio (δ13Choney, δ13Cprotein and δ15N) ranges and examine their relation with botanical origin of honey. Despite that δ13C parameter has primary purpose to detect honey adulteration, stable isotopes generally have become important parameter for detection its botanical and geographical origin. The data about stable isotopes are scarce in comparison to other well-known parameters in honey, and in Croatia there is no data about stable isotopes in unifloral honey. This research includes six characteristic honey types (black locust, chestnut, lime, rape, winter savory, and sage honey) from Croatia. Large number of differences between honey types were found in the analyzed IRMS parameters. PCA analysis has successfully separated winter savory from all other honey types, except sage honey, whose samples differed from black locust samples.
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Data availability
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- AOAC:
-
Association of official analytical collaboration (AOAC) international
- BCR:
-
Community Bureau of reference
- EA–IRMS:
-
Elemental analyser–isotope ratio mass spectrometer
- EA/LC–IRMS:
-
Elemental analyser/liquid chromatography–isotope ratio mass spectrometer
- IAEA-N:
-
International atomic energy agency
- ICP–MS:
-
Inductively coupled plasma–mass spectrometry
- IRMS:
-
Isotope ratio mass spectrometry
- ISCIRA:
-
Internal stable carbon isotope ratio analysis
- KW test:
-
Kruskal–Wallis test
- LC–MS:
-
Liquid chromatography–mass spectrometry
- MWW test:
-
Mann–Withney–Wilcoxon test
- PCA:
-
Principal component analysis
- PDB:
-
Peedee belemnite
- SCIRA:
-
Stable carbon isotope ratio analysis
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BBR: Conceptualization; Formal analysis; Writing—original draft; IF: Writing—editing; Visualization; Conceptualization; Methodology; Resource; DMO: Writing—editing; Visualization; ŽT: Writing—editing; FČK: Writing—editing; LJP: Conceptualization; Writing—editing; Visualization; Methodology; Resource.
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Bilić Rajs, B., Flanjak, I., Milojković-Opsenica, D. et al. Analysis of δ13C and δ15N values in Croatian honey by EA–IRMS and possibility of their application in botanical origin verification. J Food Sci Technol 61, 939–949 (2024). https://doi.org/10.1007/s13197-023-05888-9
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DOI: https://doi.org/10.1007/s13197-023-05888-9