Abstract
Enterococcus bacteria are studied in various sectors including fermentation, food and dairy industries,as well as studied for their probiotic properties but have limited use due to their possible pathogenic behavior. The present report talks about the metabolites produced, by the previously isolated Enterococcus strain, E.villorum SB2 (accession number KX830968), from the vaginal source. The growth of the bacteria in three types of media (M17, MRS and LAPTg) was compared, where the M17 media gave better bacterial colonies, also maximum growth rate was observed in M17 media (Td = 1.6 h & k = 0.4 h−1), and thus was selected as the metabolite production media. Further, the studied bacteria did not show any hemolytic activity, making it safe for industrial applications. The HR-LCMS results showed the production of various amino acids, organic acids, peptides, and other metabolites like flavonoids (Quercetin 3-O-Manoglucoside), terpenoids (7’,8’,Dihydro-8’-hydroxycitraniaxanthin, O-Methylganoderic acid O, Thalicsessine, Austinol, Valdiate), indole derivatives produced by tryptophan metabolism (5-hydroxykynurenamine, 2S,4R)-4-(9H-Pyrido[3,4-b]indol-1-yl)-1,2,4-butanetriol, Indoleacrylic acid), antimicrobial compounds (Fortimicin A) and fatty acids (Stearic acid, Myristic acid), which were earlier unreported form Enterococcus species opening new scope for discovering new industrial applications of the strain. As the studied bacteria has been reported to be a potential probiotic, the detection of these industrially important metabolites can be studied further in future studies to reveal the potential industrial applications of the strain.
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Acknowledgements
We want to express our special thanks to Bhagwat et al. (Bhagwat and Annapure 2019b) for providing us the isolated Enterococcus strains for the current study. We would like to express our gratitude towards DST and SAIF/CRNTS, IIT Bombay, for providing HR-LCMS analytical facility for the research work.
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All authors contributed to the study conception and design. Material preparation, data collection, analysis and manuscript drafting was performed by SSG. The guidance, comments on the manuscript draft, and approval was provided by Prof. USA. Both the authors read and approved the final manuscript.
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Gaur, S.S., Annapure, U.S. Untargeted metabolite profiling of Enterococcus villorum SB2, isolated from the vagina of pregnant women, by HR-LCMS. World J Microbiol Biotechnol 38, 219 (2022). https://doi.org/10.1007/s11274-022-03404-3
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DOI: https://doi.org/10.1007/s11274-022-03404-3