Skip to main content
SpringerLink
Log in

Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Tobacco leaf enation virus (TLEV) is a putative member of the genus Phytoreovirus within the family Reoviridae. Previous western blot analysis of structural viral proteins (apparent molecular weights of 93 kDa; 58 kDa; 48 kDa; 39 kDa and 36 kDa) associated with TLEV, isolated from infected tobacco in South Africa, suggested that these proteins may correspond to structural Wound tumor virus (WTV) proteins. To further establish the nature of this novel virus disease phenotype in tobacco, molecular characterization of six dsRNA components was undertaken. Full-length cDNA clones were obtained by an optimized modified single-primer amplification sequence-independent dsRNA cloning method. Results of this study revealed the conserved terminal sequence: 5′GG(U/C)...UGAU 3′ of segments S6–S12, while adjacent to these conserved terminal sequences are imperfect inverted repeats (7–15 bp in length), both features being common to reoviruses. The complete nucleotide sequences of segments S5 (2,610 bp), S7 (1,740 bp), S8 (1,439 bp), S10 (1,252 bp), S11 (1,187 bp) and S12 (836 bp) were determined. Comparison of full-length nucleotide sequences with corresponding segments of other phytoreoviruses, Rice gall dwarf virus (RGDV), Rice dwarf virus (RDV) and WTV has shown nucleotide and predicted amino acid identities within the range of 30–60%. TLEV consistently shows a higher identity to WTV than to other phytoreovirus species where sequence data is available. Each segment had a single predicted open reading frame encoding proteins with calculated molecular weights of S5 (90.6 kDa); S7 (58.1 kDa); S8 (47.7 kDa); S10 (39.8 kDa); S11 (35 kDa) and S12 (19.5 kDa). The relatively low nucleotide and amino acid identity to other members of the genus demonstrates that TLEV is a novel phytoreovirus, distinct from the only other reported dicotyledenous-infecting WTV and is the first phytoreovirus reported to emerge in Africa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. P.P.C. Mertens, M. Arella, H. Attoui, S. Belloncik, M. Bergoin, G. Boccardo, T.F. Booth, W. Chiu, J.M. Disprose, R. Duncan, in ed. by M.H.V. van Regenmortel, C.M. Fauquet, D.H.L. Bishop, F.B. Carstens, M.K. Estes, S.M. Lemon, J. Maniloff, M.A. Mayo, D.J. McGeogh, C.R. Pringle, R.B. Wickner. Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses, (Academic Press, San Diego, 2000), p. 395–480

  2. M.E.C. Rey, E. D’Andrea, J. Calvert-Evers, M. Paximadis, G. Boccardo, Phytopath 89, 303–307 (1999)

    Article  CAS  Google Scholar 

  3. M. Paximadis, G. Dusterwald, L. Duyver, M.E.C. Rey, Plant Pathol. 46, 282–290 (1997)

    Article  Google Scholar 

  4. L.M. Black, Am J Bot. 32, 408–415 (1945)

    Article  Google Scholar 

  5. T. Omura, Sem Virol. 6, 97–102 (1995)

    Article  CAS  Google Scholar 

  6. I. Uyeda, R.G. Milne, Sem Virol. 6, 85–88 (1995)

    Article  Google Scholar 

  7. J.V. Anzola, D.J. Dall, Z. Xu, D.L. Nuss, Virology 171, 222–228 (1989)

    Article  CAS  Google Scholar 

  8. J.V. Anzola, D.J. Dall, Z. Xu, D.L. Nuss, Nucl. Acids Res. 17, 3300 (1989)

    Article  CAS  Google Scholar 

  9. T. Asamizu, D. Summers, M.B. Motika, J.V. Anzola, D.L. Nuss, Virology 144, 398–409 (1985)

    Article  CAS  Google Scholar 

  10. D.J. Dall, J.V. Anzola, Z. Xu, D.L. Nuss, Nucl. Acids Res. 17, 3599 (1989)

    Article  CAS  Google Scholar 

  11. D.J. Dall, J.V. Anzola, Z. Xu, D.L. Nuss, Virology 179, 599–608 (1990)

    Article  CAS  Google Scholar 

  12. K. Ichimi, A. Kikuchi, Y. Moriyasu, X.B. Zhong, K. Hagiwara, H. Kamiunten, T. Omura, JARQ 36, 83–87 (2002)

    Article  CAS  Google Scholar 

  13. H. Koganezawa, H. Hibino, F. Motoyoshi, H. Kato, H. Noda, K. Ishikawa, T. Omura, J. Gen. Virol. 71, 1861–1863 (1990)

    Article  CAS  Google Scholar 

  14. Y. Moriyasu, K. Ishikawa, A. Kikuchi, S. Imanishi, S. Tomita, K. Akutsu, T. Omura, Virus Genes 20, 237–241 (2000)

    Article  CAS  Google Scholar 

  15. H. Noda, K. Ishikawa, H. Hibino, H. Kato, T. Omura, J. Gen. Virol. 72, 2837–2842 (1991)

    Article  CAS  Google Scholar 

  16. N. Suzuki, Sem. Virol. 6, 89–95 (1995)

    Article  CAS  Google Scholar 

  17. B.X. Zhong, A. Kikuchi, Y. Moriyasu, T. Higashi, T. Hagiwara, T. Omura, Arch. Virol. 148, 2275–2280 (2003)

    Article  CAS  Google Scholar 

  18. Y. Li, Y.M. Bao, C.H. Wei, K.S. Kang, Y.W. Zhong, P. Mao, G. Wu, Z.L. Chen, J. Schiemann, R.S. Nelson, J. Virol. 78, 5382–5389 (2004)

    Article  CAS  Google Scholar 

  19. N. Miyazaki, K. Hagiwara, H. Naitow, T. Higashi, R.H. Cheng, T. Tsukihara, A. Nakagawa, T. Omura, J. Mol. Biol. 345, 229–237 (2005)

    Article  CAS  Google Scholar 

  20. T. Omura, K. Ishikawa, H. Hirano, M. Ugaki, Y. Minobe, T. Tsuchizaki, H. Kato, J. Gen. Virol. 70, 2759–2764 (1989)

    Article  CAS  Google Scholar 

  21. N. Suzuki, M. Sugawara, T. Kusano, Virology 191, 992–995 (1992)

    Article  CAS  Google Scholar 

  22. S. Ueda, I. Uyeda, Mol. Plant Pathol. On-Line [http://www.bspp.org.uk/mppo1], (1997)

  23. Z. Xu, J.V. Anzola, D.L. Nuss, Virology 168, 73–78 (1989)

    Article  CAS  Google Scholar 

  24. B.X. Zhong, Y.W. Shen, T. Omura, Acta Biochim. Biophys. Sin. 37, 55–60 (2005)

    Article  CAS  Google Scholar 

  25. S. Zhu, F. Gao, X. Cao, M. Chen, G. Ye, C. Wei, Y. Li, Plant Physiol. 139, 1935–1945 (2005)

    Article  CAS  Google Scholar 

  26. Y. Wei, A. Kikuchi, N. Suzuki, T. Shimizu, K. Hagiwara, H. Chen, T. Omura, Arch. Virol. 151, 1701–1712 (2006)

    Article  CAS  Google Scholar 

  27. Y. Takahashi, M. Tomiyama, H. Hibino, T. Omura, J. Gen. Virol. 75, 269–275 (1994)

    Article  CAS  Google Scholar 

  28. B.I. Hillman, J.V. Anzola, B.T. Halpern, T.D. Cavileer, D.L. Nuss, Virology 185, 896–900 (1991)

    Article  CAS  Google Scholar 

  29. T.J. Morris, J.A. Dodds, Phytopathology 69, 854–858 (1979)

    Article  CAS  Google Scholar 

  30. A.C. Potgieter, A.D. Steele, A.A. van Dijk, J. Gen. Virol. 83, 2215–2223 (2002)

    Article  CAS  Google Scholar 

  31. C.T. Chung, R.H. Miller, Nucl. Acids Res. 16, 3580 (1988)

    Article  CAS  Google Scholar 

  32. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucl. Acids. Res. 22, 4673–4680 (1994)

    Article  CAS  Google Scholar 

  33. N. Saitou, M. Nei, Mol. Biol. Evol. 4, 406–425 (1987)

    CAS  Google Scholar 

  34. D. Swofford, PAUP: Phylogenetic Analysis Using Parsimony, version 3.1.1. (University of Illinois, Natural History Survey, Champaign, IL, 1998)

    Google Scholar 

  35. J.V. Anzola, Z. Xu, T. Asamizu, D.L. Nuss, Proc. Nat. Acad. Sci. USA 84, 8301–8305 (1987)

    Article  CAS  Google Scholar 

  36. X. Cao, P. Zhou, X. Zhang, S. Zhu, X. Zhong, Q. Xiao, B. Ding, Y. Li, J. Virol. 79, 13018–13027 (2005)

    Article  CAS  Google Scholar 

  37. H. Xu, Y. Li, Z.J. Mao, Z.L. Chen, Virology 240, 267–272 (1998)

    Article  CAS  Google Scholar 

  38. N. Suzuki, D. Hosokawa, Y. Matsuura, A. Kituchi, T. Omura, Arch. Virol. 144, 1371–1380 (1999)

    Article  CAS  Google Scholar 

  39. T. Wei, T. Shimizu, K. Hagiwara, A. Kikuchi, Y. Moriyasu, N. Suzuki, H. Chen, T. Omura, J. Gern. Virol. 87, 429–438 (2006)

    Article  CAS  Google Scholar 

  40. W.R. Pearson, Methods Enzymol. 183, 63–98 (1990)

    Article  CAS  Google Scholar 

  41. N. Nakashima, H. Noda, Virology 107, 303–307 (1995)

    Article  Google Scholar 

  42. H.H. Storey, Nature 128, 187–188 (1931)

    Article  Google Scholar 

Download references

Acknowledgements

The authors thank Dr. Maria Paximadis for her assistance, Dr. Brad Hillman for supplying WTV-infected and healthy clover samples and Dr. Judy Brown for assistance in some of the phylogenetic tree construction programs.

Author information

Authors and Affiliations

  1. School of Molecular and Cell Biology, University of the Witwatersrand, Private Bag 3, Johannesburg, PO Wits 2050, South Africa

    Anabela Picton & Marie Emma Christine Rey

  2. Biochemistry Division, Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa

    Christiaan Potgieter

Authors
  1. Anabela Picton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christiaan Potgieter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marie Emma Christine Rey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marie Emma Christine Rey.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Picton, A., Potgieter, C. & Rey, M.E.C. Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco. Virus Genes 35, 387–393 (2007). https://doi.org/10.1007/s11262-007-0088-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-007-0088-x

Keywords

Search

Navigation