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KGF Phage Model Peptide Accelerates Cutaneous Wound Healing in a Diabetic Rat Model

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Abstract

The authors have previously demonstrated that one phage-displayed keratinocyte growth factor (KGF) model peptide that can bind to epidermal cells and facilitate cellular proliferation. In this study, the authors investigated the role of phage-displayed KGF model peptide on wound healing in diabetic rats. Full-thickness excisional dorsal wounds were created in the diabetic rats, after which the rats were randomly divided into five groups: negative control group [normal saline (NS)], two KGF control groups which were respectively treated with low-dose KGF (5 ng/mL, K-LD) and high-dose KGF (50 ng/mL, K-HD), and two KGF model-peptide-treated groups which were respectively treated with low-dose model peptide (5 ng/mL, M-LD) and high-dose model peptide (50 ng/mL, M-HD). On day 14 post-injury, wound closure was followed by digital planimetry and wound tissues were harvested for histologic assay and real-time polymerase chain reaction. Wounds treated with model peptide closed markedly faster than negative control wounds and were comparable to KGF treated wounds. Histology and immunohistology results demonstrated significantly higher levels of re-epithelization, granulation tissue formation and vascularization in the model peptide groups. Furthermore, real-time polymerase chain reaction expression of KGFR, collagen I and transforming growth factor-β1 in model peptide groups, were also generally higher than that in negative control group. Phage-displayed KGF peptide promotes wound healing through accelerating re-epithelialization, enhancing dermal regeneration, and inducing angiogenesis. Model peptide possesses the potential to be a promising therapeutic option for the treatment of diabetic ulcers.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (30670571, 81201467), and the Scientific Research Fund for Youth of Chinese Academy of Medical Sciences and Peking Union Medical College (2017310007).

Funding

This study was supported by the National Natural Science Foundation of China (30670571, 81201467), and the Scientific Research Fund for Youth of Chinese Academy of Medical Sciences and Peking Union Medical College (2017310007).

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Authors and Affiliations

  1. 16th Department, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Hong Du, Guodong Song, Dong Zhang, Panxi Yu, Xiaolei Jin & Xianlei Zong

  2. Department of Scientific Research, Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Chunyan Cao

Authors
  1. Hong Du
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  2. Guodong Song
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  3. Chunyan Cao
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  4. Dong Zhang
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  5. Panxi Yu
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  6. Xiaolei Jin
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  7. Xianlei Zong
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Correspondence to Xiaolei Jin or Xianlei Zong.

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The authors declare that there are no conflicts of interest.

Ethical Approval

The current research was approved by the Medical Ethical Committee at Plastic Surgery Hospital of Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

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In this type of study, formal consent is not required.

Research Involving Human and/or Animal Participants

50 female Sprague–Dawley (SD) rats were obtained from Beijing Medical Laboratory Animal Center (SYXK 2020–0018). This article does not contain any studies with human participants performed by any of the authors.

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Du, H., Song, G., Cao, C. et al. KGF Phage Model Peptide Accelerates Cutaneous Wound Healing in a Diabetic Rat Model. Int J Pept Res Ther 27, 1769–1781 (2021). https://doi.org/10.1007/s10989-021-10209-9

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  • DOI: https://doi.org/10.1007/s10989-021-10209-9

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