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The effect of cryopreservation or heating on the mechanical properties and histomorphology of rat bone-patellar tendon-bone

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Abstract

The effects of cryopreservation on tendon allograft have been reported, but remain unclear, particularly the potential effects on mechanical properties and histological changes by ice crystal formation. There are also few studies about effects of heating for sterilization of tendon. We evaluated the effect of cryopreservation or heating on the mechanical properties and histomorphology of rat bone-patellar tendon-bones (BTBs). BTBs were processed by cryopreservation at −80°C for 3 weeks, or heating at 80°C for 10 min. Tensile testing and histomorphological examination were performed. The cryopreservation of tendons showed less influences on their mechanical properties. When cryopreserved BTBs in frozen state were fixed by freeze-substitution method, many spaces were observed in interfibrillar substances. These results suggest that the collagen fibers of cryopreserved tendons were histomorphologically affected by ice crystals. The heating of tendons completely destroyed the collagen fibers of the tendons and is therefore thought to be inappropriate for the sterilization of BTBs.

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Acknowledgment

We would like to thank K. Kamiya for help with statistical analysis, and N. Watanabe for help with biomechanical tests.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Kitasato University School of Medicine, 15-1 Kitasato 1-chome, 228-8555, Sagamihara, Kanagawa, Japan

    Hwang Jung Park, Ken Urabe, Kouji Naruse, Kenji Onuma & Moritoshi Itoman

  2. Research Center for Biological Imaging, Kitasato University School of Medicine, 15-1 Kitasato 1-chome, 228-8555, Sagamihara, Kanagawa, Japan

    Noriko Nemoto

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  1. Hwang Jung Park
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  2. Ken Urabe
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  3. Kouji Naruse
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  4. Kenji Onuma
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  5. Noriko Nemoto
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  6. Moritoshi Itoman
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Correspondence to Ken Urabe.

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Park, H.J., Urabe, K., Naruse, K. et al. The effect of cryopreservation or heating on the mechanical properties and histomorphology of rat bone-patellar tendon-bone. Cell Tissue Bank 10, 11–18 (2009). https://doi.org/10.1007/s10561-008-9109-9

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  • DOI: https://doi.org/10.1007/s10561-008-9109-9

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