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Saccharothrix lopnurensis sp. nov., a filamentous actinomycete isolated from sediment of Lop Nur

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Abstract

A novel actinomycete strain, designated YIM LPA2hT, was isolated from a sediment sample collected from Lop Nur, Xinjiang Uygur Autonomous Region, North–West China. The taxonomic position of strain YIM LPA2hT was investigated by a polyphasic approach. The morphological and chemotaxonomic properties of the isolate were in accordance with the members of the genus Saccharothrix. The 16S rRNA gene sequence of the strain showed highest similarities to Saccharothrix yanglingensis (98.6 %), Saccharothrix longispora (98.4 %) and Saccharothrix hoggarensis (98.3 %). However, the DNA–DNA hybridization values between the new isolate YIM LPA2hT and S. yanglingensis, S. longispora and S. hoggarensis were significantly below 70 %. Strain YIM LPA2hT was found to contain meso-diaminopimelic acid as diagnostic diamino acid. The major sugars in whole-cell hydrolysates were rhamnose, galactose, mannose, glucose and fructose. The major polar lipids were identified as phosphatidylethanolamine, phosphatidylhydroxylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. The predominant respiratory menaquinones were MK-9 (H4) and MK-10 (H4). The major fatty acids were C17:1 ω8c (15 %), iso-C15:0 (12 %), anteiso-C15:0 (12 %) and summed feature 3 (C16:1 ω6c/C16:1 ω7c, 10 %). The genomic DNA G+C content of strain YIM LPA2hT was determined to be 75 mol %. The genotypic and phenotypic results suggest that strain YIM LPA2hT represents a novel species of the genus Saccharothrix, for which the name Saccharothrix lopnurensis sp. nov. is proposed. The type strain is YIM LPA2hT (=CGMCC 4.7246T=KCTC 39545T).

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Acknowledgments

The authors are grateful to Prof. Yu-Guang Zhou (CGMCC, China) and Dr. Ruediger Pukall (DSMZ, Germany) for providing the reference type strains. This research work was supported by Chinese National Program of High Technology Research and Development (2012AA021705) and Natural Science Foundation of Xinjiang Uygur Autonomous Region (No. 2011211A030). WJ Li was also supported by the Hundred Talents Program of Chinese Academy of Sciences and Guangdong Province Higher Vocational Colleges and Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Ürümqi, 830054, People’s Republic of China

      Yu-Qian Li, Cong Cheng, Xiao-Han Shi, Chun-Yan Lu & Deng-Di An

    2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Lan Liu & Wen-Jun Li

    3. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Zhou-Yan Dong, Nimaichand Salam & Wen-Jun Li

    4. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, Xinjiang, People’s Republic of China

      Wen-Jun Li

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    1. Yu-Qian Li
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    Yu-Qian Li and Lan Liu have contributed equally to this work.

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    Li, YQ., Liu, L., Cheng, C. et al. Saccharothrix lopnurensis sp. nov., a filamentous actinomycete isolated from sediment of Lop Nur. Antonie van Leeuwenhoek 108, 975–981 (2015). https://doi.org/10.1007/s10482-015-0550-z

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