Abstract
The long-term cryopreservation of the germplasm (spermatozoa and oocytes) of threatened species offers flexibility in their genetic management and security against disasters or outbreaks of disease that might seriously affect subpopulations. A wide range of infectious diseases can, however, be transmitted via artificial insemination, and the risk of infectious diseases being spread via manipulated germplasm needs to be carefully managed in order to avoid fertility problems in the dams and decreased survival of offspring. Furthermore, accidental introduction of exotic microorganisms into ecosystems remain a major threat. The aims of the present study were to assess the impact of diseases transmissible by sperm on the establishment of germplasm banks for the Iberian ibex, a wild mountain ungulate, and to determine the influence of the presence of these pathogens on sperm functionality. Blood and sperm samples were obtained from 52 mature ibex males legally shot in southern Spain. Sperm motility, morphological abnormalities, acrosome integrity and plasma membrane integrity were assessed for each sample to determine in vitro sperm quality. All serum samples underwent serological analysis for bovine herpes virus type I, bluetongue virus (BTV), bovine leukaemia virus, caprine arthritis–encephalitis virus, pestivirus, Brucella, Coxiella burnetii, Chlamydiophila abortus, Mycoplasma agalactiae and Borrelia burgdorferi. The highest prevalence (30.7%) was recorded for B. burgdorferi, followed by C. burnetii (13.4%). A total of 734 sperm doses containing approximately 200 × 106 spermatozoa each were frozen in straws. Forty-five straws (6.1% of the total number) came from animals seropositive for diseases listed in the Terrestrial Animal Code of the OIE for collection and processing of bovine and small ruminant semen (in this case, pestivirus and BTV). A total of 271 frozen straws (36.9% of the total of frozen straws) were provided by animals seropositive for pathogens potentially transmissible by semen not included in the above OIE code (in this case, B. burgdorferi and C. burnetii). The values of sperm variables were not affected by seropositivity for any of the pathogens transmissible by sperm.
Similar content being viewed by others
References
Afshar A, Eaglesome MD (1990) Viruses associated with bovine serum. Vet Bull 60:93–109
Amin AS, Hamdy MER, Ibrahim AK (2001) Detection of Brucella melitensis in semen using the polymerase chain reaction assay. Vet Microbiol 83:37–44
Bach G (2001) Recovery of Lyme spirochetes by PCR in semen samples previously diagnosed Lyme disease patients. 14th International Scientific Conference on Lyme Disease, April 2001. Hartford, CT.
Burgess EC, Wachal MD, Cleven TD (1993) Borrelia burgdorferi infection in dairy cows, rodents, and birds from four Wisconsin dairy farms. Vet Microbiol 35:61–77
Calvete C, Miranda MA, Estrada R, Borras D, Sarto i Monteys V, Collantes F, García de Francisco JM, Moreno N, Lucientes J (2006) Spatial distribution of Culicoides imicola, the main vector of bluetongue virus, in Spain. Vet Rec 158:130–131
Campbell RC, Dott HM, Glover TD (1956) Nigrosin eosin as a stain for differentiating live and dead spermatozoa. J Agric Sci 48:1–8
Clarke GN (1999) Sperm cryopreservation: is there a significant risk of cross-contamination? Hum Reprod 14:2941–2943
de la Fe C, Amores J, Gómez Martín A, Sánchez A, Contreras A, Corrales JC (2009) Mycoplasma agalactiae detected in the semen of goat bucks. Theriogenology 72:1278–1281
Fandos P (1991) Evolución de la población de cabra montés en el Parque Natural de Cazorla, Segura y las Villas. In: ICONA-CSIC (eds) La cabra montés en el Parque Natural de las Sierras de Cazorla, Segura y las Villas. Madrid, Spain, pp 123–133
Foster NM, Alders MA, Luedke AJ, Walton TE (1980) Abnormalities and virus-like particles in spermatozoa from bulls latency infected by bluetongue virus. Am J Vet Res 41:1045–1048
Garcia I, Napp S, Casal J, Perea A, Allepuz A, Alba A, Carbonero A, Arenas A (2009) Bluetongue epidemiology in wild ruminants from Southern Spain. Eur J Wildl Res 55:173–178
Gustafson JM (1993) The in utero and seminal transmission of Borrelia burgdorferi in Canidae. PhD Thesis, University of Wisconsin, Madison, USA.
Gustafson JM, Burgess EC, Wachal MD, Steinberg H (1993) Intrauterine transmission of Borrelia burgdorferi in dogs. Am J Vet Res 54:882–890
Harvey WT, Salvato P (2003) Lyme disease: ancient engine of an unrecognized borreliosis pandemic? Med Hypotheses 60:742–759
Holt WV (1994) Reproductive technologies. In: Olney PJS, Mace GM, Feistner ATC (eds) Creative conservation: interactive management of wild and captive animals. Chapman and Hall, London, pp 144–166
Hrudka F (1984) Microscopical evidence of Mycoplasma-like organisms in Mycoplasma positive bovine semen. Andrologia 16:203–212
Jeyendran R, Van Der Ven H, Perez-Pelaez M, Crabo B, Zaneveld L (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relation to other semen characteristics. J Reprod Fertil 70:219–228
Johnson RC, Marek N, Kodner C (1984) Infection of Syrian hamsters with Lyme diseases spirochetes. J Clin Microbiol 20:1099–1101
Kirkwood JK, Colenbrander B (2001) Disease control measures for genetic resource banking. In: Watson PF, Holt WV (eds) Cryobanking the genetic resource: wildlife conservation for the future? Taylor and Francis, London, pp 69–84
Kirschvink N, Raes M, Saegerman C (2009) Impact of a natural bluetongue serotype 8 infection on semen quality of Belgian rams in 2007. Vet J 182:244–251
Komarov A, Goldsmit L (1951) A disease similar to bluetongue in cattle and sheep in Israel. Refuah Vet 8:96–100
Kruszewska D, Tylewska-Wierzbanowska SK (1993) Coxiella burnetii penetration into the reproductive system of male mice, promoting sexual transmission of infection. Infect Immun 61:4188–4195
Kruszewska D, Lembowicz K, Tylewska-Wierzbanowska S (1996) Possible sexual transmission of Q fever among humans. Clin Infect Dis 22:1087–1088
Kumi-Diaka J, Harris O (1994) Effects of Borrelia burgdorferi organisms on the functional characteristics and membrane integrity of the canine spermatozoa. Vet Hum Toxicol 36:281–285
Kumi-Diaka J, Harris O (1995) Viability of Borrelia burgdorferi in stored semen. Br Vet J 151:221–224
Leibstein MM, Khan MI, Bushmich SL (1998) Evidence for in utero transmission of Borrelia burgdorferi from naturally infected cows. J Spirochetal Tick-Borne Dis 5:54–62
Lozano EA (1986) Etiologic significance of bacterial isolates from rams with palpable epididymitis. Am J Vet Res 47:1153–1156
Lucas MH, Dawson M, Chasey D, Wibberley G, Roberts DH (1980) Enzootic bovine leukosis virus in semen. Vet Rec 106:128
MacLachlan NJ, Osburn BI (2006) Impact of bluetongue virus infection on the international movement and trade of ruminants. J Am Vet Med Assoc 228:1346–1349
Mellor P, Wittmann EJ (2002) Bluetongue virus in the Mediterranean basin, 1998–2001. Vet J 164:20–37
Meyling A, Jensen AM (1988) Transmission of bovine virus diarrhoea virus (BVDV) by artificial insemination (AI) with semen from a persistently-infected bull. Vet Microbiol 17:97–105
OIE, World Organisation for Animal Health (2008) Manual of diagnostic tests and vaccines for terrestrial animals, 6th edn. OIE, Paris, France
OIE, World Organisation for Animal Health (2009) Terrestrial Animal Health Code 2009. Chapter 4.5. Collection and processing of bovine, small ruminant and porcine semen. http://www.oie.int/eng/normes/mcode/en_chapitre_1.8.3.htm
Osburn BI (1994) The impact of bluetongue virus on reproduction. Comp Immunol Microbiol Infect Dis 17:189–196
Paton DJ, Goodey R, Brockman S, Wood L (1989) Evaluation of the quality and virological status of semen from bulls acutely infected with BVDV. Vet Rec 124:63–64
Philpott M (1993) The dangers of disease transmission by artificial insemination and embryo transfer. Br Vet J 149:339–369
Pursel V, Johnson L (1974) Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology 1:63–68
Revell SG, Chasey D, Drew TW, Edwards S (1988) Some observations on the semen of bulls persistently infected with bovine diarrhoea virus. Vet Rec 123:122–125
Robison CD, Davis DS, Templeton JW, Westhusin M, Foxworth WB, Gilsdorf MJ, Adams LG (1998) Conservation of germplasm from bison infected with Brucella abortus. J Wildl Dis 34:582–589
Russell PH, Lyaruu VH, Millar JD, Curry MR, Watson PF (1997) The potential transmission of infectious agents by semen packaging during storage for artificial insemination. Anim Reprod Sci 47:337–342
Santiago-Moreno J, Toledano-Díaz A, Dorado J, Pulido-Pastor A, Coloma MA, López-Sebastián A (2007) Recovery and cryopreservation of Spanish Ibex epididymal spermatozoa. Arch Androl 53:309–316
Santiago-Moreno J, Astorga RJ, Luque I, Coloma MA, Toledano-Díaz A, Pulido-Pastor A, Gómez-Guillamon F, Salas-Vega R, López-Sebastián A (2009) Influence of recovery method and microbial contamination on the response to freezing–thawing in ibex (Capra pyrenaica) epididymal spermatozoa. Cryobiology 59:357–362
Smith MC, Sherman DM (2009) Goat medicine, 2nd edn. Wiley-Blackwell, Iowa
StatSoft (1995) STATISTICA® for Windows. version 5.0 [computer program]. Tulsa, OK: StatSoft Inc
Tedder RS, Zuckerman MA, Goldstone AH, Hawkins AE, Fielding A, Briggs EM, Irwin D, Blair S, Gorman AM, Patterson KG, Linch DC, Heptonstall J, Brink NS (1995) Hepatitis B transmission from contaminated cryopreservation tank. Lancet 346:137–140
Travasos CE, Benoit C, Valas S, da Silva AG, Perrin G (1999) Caprine arthritis–encephalitis virus en semen of naturally infected bucks. Small Rumin Res 32:101–106
Acknowledgements
This work was funded by FEDER-INIA (grant FAU 2006-00001). The authors would like to thank the Sedella District Council (Málaga) for its help in setting up the local laboratory, the gamekeepers of the Tejeda and Almijara Game Reserve for help in obtaining samples and the Regional Environmental Council of Málaga (Consejería de Medio Ambiente, Junta de Andalucía) for its unfailing help in the execution of all projects proposed.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by C. Gortázar
Rights and permissions
About this article
Cite this article
Santiago-Moreno, J., Carvajal, A., Astorga, R.J. et al. Potential impact of diseases transmissible by sperm on the establishment of Iberian ibex (Capra pyrenaica) genome resource banks. Eur J Wildl Res 57, 211–216 (2011). https://doi.org/10.1007/s10344-010-0413-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10344-010-0413-x