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Quality Control of Xiebai San Standard Decoction Assisted by Network Pharmacology Strategy

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Abstract

Xiebai San (XBS) is a classical herbal prescription which has been used for the treatment of infantile cough and asthma for several hundred years. In this study, 39 compounds in XBS standard decoction were identified by HPLC–ESI–TOF system. Network pharmacology strategy was adopted to explore the relationship of chemicals, target genes and pathways, mulberroside A, kukoamine B and glycyrrhizic acid were chosen as quality markers in XBS standard decoction. An ultra-high-performance liquid chromatography (UHPLC) method was established to simultaneously determine these three compounds on the Waters CORTECS T3 column (150 mm × 3 mm, 1.6 µm) at 0.45 mL min−1 with 0.1% (v/v) TFA (A) and acetonitrile (B) as the mobile phase. The developed method was validated with satisfactory parameters: specificity, linearity, precision, accuracy, stability and ruggedness. The method was then applied to analyze ten batches of XBS standard decoction and the initial quality control standard of XBS standard decoction was established.

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Acknowledgements

This research was funded by Grant of National Natural Science Funds of China (Grant No. 81973464/H2803), National Key R&D Program of China (Grant No. U1508220), National Natural Science Foundation of China (Grant No.U1508220), Guidance Plan of Natural Science Foundation of Liaoning in China (2019-ZD-0463) and supported by Liaoning Distinguished Professor Project for Qing Li (2017).

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Author notes

  1. Wenchuan Bi and Yan Cui are two corresponding units contributed equality to this work.

Authors and Affiliations

  1. School of Pharmacy, National and Local Joint Engineering Laboratory for Key Technology of Chinese Material Medical Quality Control, Shenyang Pharmaceutical University, 103 Wenhua Rd, Shenyang, 110016, China

    Wenhua Zhang, Xiuli Sun, Xiaoyan Jiang, Song Cang, Qi Wang, Ran Liu, Huarong Xu, Qing Li & Yan Cui

  2. Shenyang Harmony Health Medical Laboratory and Key Laboratory of Shenyang Medical Mass Spectrometry Technology, No. 19, Wenhui Street, Hunnan District, Shenyang, 110179, China

    Wenwen Sui

  3. School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, China

    Wenchuan Bi

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  1. Wenhua Zhang
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Correspondence to Wenchuan Bi or Yan Cui.

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Zhang, W., Sun, X., Sui, W. et al. Quality Control of Xiebai San Standard Decoction Assisted by Network Pharmacology Strategy. Chromatographia 83, 873–884 (2020). https://doi.org/10.1007/s10337-020-03897-w

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