Skip to main content
SpringerLink
Log in

Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Two double stranded RNAs (dsRNAs) that likely represent the genome of an alphapartitivirus, tentatively named "impatiens cryptic virus 1" (ICV1), were recovered from Impatiens balsamina L. RNA1 (2008 bp) codes for the RNA-dependent RNA polymerase (RdRp) of ICV1, which shares <83% amino acid sequence identity with the RdRps of other alphapartitiviruses. RNA2 (1906 bp) codes for the coat protein (CP) of ICV1, which shares <60% amino acid sequence identity with the CPs of other alphapartitiviruses. Phylogenetic analysis suggested that ICV1 is closely related to plant alphapartitiviruses, including vicia cryptic virus, beet cryptic virus 1, carrot cryptic virus, and white clover cryptic virus 1. Using primers specific for RNA1 or RNA2, ICV1 could be detected in I. balsamina from various parts of China.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Vainio EJ, Chiba S, Ghabrial SA, Maiss E, Roossinck M, Sabanadzovic S, Suzuki N, Xie J, Nibert M, ICTV Report Consortium (2018) ICTV virus taxonomy profile: Partitiviridae. J Gen Virol 99:17–18

    Article  CAS  Google Scholar 

  2. Nibert ML, Ghabrial SA, Maiss E, Lesker T, Vainio EJ, Jiang D, Suzuki N (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188:128–141

    Article  CAS  Google Scholar 

  3. Boccardo G, Lisa V, Luisoni E, Milne RG (1987) Cryptic plant viruses. Adv Virus Res 32:171–214

    Article  CAS  Google Scholar 

  4. Boccardo G, Candresse T (2005) Complete sequence of the RNA2 of an isolate of white clover cryptic virus 1, type species of the genus Alphacryptovirus. Arch Virol 150:403–405

    Article  CAS  Google Scholar 

  5. Suzuki A, Kobayashi F, Abe M, Uchiumi T, Higashi S (2001) Cloning and expression of a down-regulated gene (TrEnodDR1) of white clover responded by thenod genes derived from Rhizobium leguminosarum bv. trifolii strain 4S. Gene 266:77–84

    Article  CAS  Google Scholar 

  6. Nakatsukasa-Akune M, Yamashita K, Shimoda Y, Uchiumi T, Abe M, Aoki T, Kamizawa A, Ayabe S, Higashi S, Suzuki A (2005) Suppression of root nodule formation by artificial expression of the TrEnodDR1 (coat protein of white clover cryptic virus 1) gene in Lotus japonicus. Mol Plant Microbe Interact 18:1069–1080

    Article  CAS  Google Scholar 

  7. Safari M, Ferrari MJ, Roossinck MJ (2019) Manipulation of aphid behavior by a persistent plant virus. J Virol 93:e01781-e1818

    Article  CAS  Google Scholar 

  8. Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  9. Guo L, Yang X, Wu W, Tan G, Fang S, Zhang S, Li F (2016) Identification and molecular characterization of Panax notoginseng virus A, which may represent an undescribed novel species of the genus Totivirus, family Totiviridae. Arch Virol 161:731–734

    Article  CAS  Google Scholar 

  10. Blawid R, Stephan D, Maiss E (2007) Molecular characterization and detection of vicia cryptic virus in different Vicia faba cultivars. Arch Virol 152:1477–1488

    Article  CAS  Google Scholar 

  11. Szegő A, Enünlü N, Deshmukh SD, Veliceasa D, Hunyadi-Gulyás É, Kühne T, Ilyés P, Potyondi L, Medzihradszky K, Lukács N (2010) The genome of beet cryptic virus 1 shows high homology to certain cryptoviruses present in phylogenetically distant hosts. Virus Genes 40:267–276

    Article  Google Scholar 

  12. Willenborg J, Menzel W, Vetten HJ, Maiss E (2009) Molecular characterization of two alphacryptovirus dsRNAs isolated from Daucus carota. Arch Virol 154:541–543

    Article  CAS  Google Scholar 

  13. Lu S, Wang J, Chitsaz F, Derbyshire MK, Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Marchler GH, Song JS, Thanki N, Yamashita RA, Yang M, Zhang D, Zheng C, Lanczycki CJ, Marchler-Bauer A (2020) CDD/SPARCLE: the conserved domain database in 2020. Nucleic Acids Res 48(D1):D265–D268

    Article  CAS  Google Scholar 

  14. Nguyen LT, Schmidt HA, Von Haeseler A, Quang MB (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 32:268–274

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the Science and Technology Project of Guizhou Tobacco Company (No. 201921) and the National Natural Science Foundation of China (No. 31972243).

Author information

Author notes

  1. Yun Zheng and Daoshun Zhang have contributed equally to this work.

Authors and Affiliations

  1. Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, 434025, Hubei, China

    Yun Zheng, Daoshun Zhang, Haoran Wang, Yan Wei, Hongliu An, Shouguo Fang & Songbai Zhang

  2. Zunyi City Company, Guizhou Tobacco Company, Zunyi, 563000, Guizhou, China

    Jianyu Gou & Xiaoyan Wang

  3. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310021, Zhejiang, China

    Haoran Wang

Authors
  1. Yun Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daoshun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Haoran Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yan Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongliu An
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jianyu Gou
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shouguo Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xiaoyan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Songbai Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Haoran Wang, Xiaoyan Wang or Songbai Zhang.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zheng, Y., Zhang, D., Wang, H. et al. Molecular characterization of a putative alphapartitivirus from Impatiens balsamina L. Arch Virol 167, 2099–2102 (2022). https://doi.org/10.1007/s00705-022-05537-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-022-05537-0

Search

Navigation