Abstract
The purpose of the present work was to investigate the concentration ranges of H2O2 generated from cigarette smoke of selected Chinese brands and the effect of different smoking behaviors on the deliveries of H2O2. A simple, rapid and reliable fluorescence assay was developed for the measurement of H2O2 based on the catalytic activity of hemoglobin using 10-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red) as the substrate. The proposed method was applied to determine the H2O2 deliveries from mainstream cigarette smoke of 30 Chinese (5 blended and 25 Virginia) and two reference cigarettes (3R4F and 1R5F) under two machine smoking regimes. Results showed that, under ISO smoking conditions, Chinese blended cigarettes had lower H2O2 yields than Chinese flue-cured cigarettes (mean value 2.41 vs. 4.69 μg cig−1, p < 0.05). As expected, the deliveries of H2O2 for all cigarettes analyzed were higher under Canadian intense smoking conditions than those under ISO conditions (ISO mean 4.31 μg cig−1 vs. Canadian mean 5.96 μg cig−1, p < 0.05). In addition, the ratios of H2O2 to nicotine yields were utilized to show the relative differences in yields among brands tested in this work and more than a twofold difference was found for most of selected Chinese blended and flue-cured cigarettes.
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Gluck M, Ehrhart J, Jayatilleke E, Zeevalk GD (2002) Inhibition of brain mitochondrial respiration by dopamine: involvement of H2O2 and hydroxyl radicals but not glutathione–protein–mixed disulfides. J Neurochem 82(1):66–74
Stone JR, Yang S (2006) Hydrogen peroxide: a signaling messenger. Antioxid Redox Sign 8(3–4):243–270
Geiszt M, Leto TL (2004) The Nox family of NAD(P)H oxidases: host defense and beyond. J Biol Chem 279(50):51715–51718
Cai H (2005) NAD (P) H oxidase-dependent self-propagation of hydrogen peroxide and vascular disease. Circ Res 96(8):818–822
Sadrzadeh S, Graf E, Panter S, Hallaway P, Eaton J (1984) Hemoglobin. A biologic fenton reagent. J Biol Chem 259(23):14354–14356
Yan F, Williams S, Griffin GD, Jagannathan R, Plunkett SE, Shafer KH, Vo-Dinh T (2005) Near-real-time determination of hydrogen peroxide generated from cigarette smoke. J Environ Monit 7(7):681–687
Hu Y, Zhang Z, Yang C (2007) The determination of hydrogen peroxide generated from cigarette smoke with an ultrasensitive and highly selective chemiluminescence method. Anal Chim Acta 601(1):95–100
Takanami Y, Moriyama T, Kosaka Y, Nakayama T (2009) Analysis of hydrogen peroxide in an aqueous extract of cigarette smoke and effect of pH on the yield. Biosci Biotech Bioch 73(10):2222–2225
Yamaguchi S, Kishikawa N, Ohyama K, Ohba Y, Kohno M, Masuda T, Takadate A, Nakashima K, Kuroda N (2010) Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species. Anal Chim Acta 665(1):74–78
Lazrus AL, Kok GL, Lind JA, Gitlin SN, Heikes BG, Shetter RE (1986) Automated fluorometric method for hydrogen peroxide in air. Anal Chem 58(3):594–597
Jie N, Yang J, Huang X, Zhang R, Song Z (1995) Fluorimetric determination of hydrogen peroxide in water using acetaminophen. Talanta 42(11):1575–1579
Zhang G, Dasgupta PK (1992) Hematin as a peroxidase substitute in hydrogen peroxide determinations. Anal Chem 64(5):517–522
Li J, Dasgupta PK (2000) Measurement of atmospheric hydrogen peroxide and hydroxymethyl hydroperoxide with a diffusion scrubber and light emitting diode-liquid core waveguide-based fluorometry. Anal Chem 72(21):5338–5347
Luo W, Li YS, Yuan J, Zhu L, Liu Z, Tang H, Liu S (2010) Ultrasensitive fluorometric determination of hydrogen peroxide and glucose by using multiferroic BiFeO3 nanoparticles as a catalyst. Talanta 81(3):901–907
Wei H, Wang E (2008) Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem 80(6):2250–2254
Adam T, McAughey J, Mocker C, McGrath C, Zimmermann R (2010) Influence of filter ventilation on the chemical composition of cigarette mainstream smoke. Anal Chim Acta 657(1):36–44
Purkis SW, Cahours X, Rey M, Teillet B, Troude V, Verron T (2011) Some consequences of using cigarette machine smoking regimes with different intensities on smoke yields and their variability. Regul Toxicol Pharm 59(2):293–309
ISO 3308, Routine analytical cigarette-smoking machine-definitions and standard conditions. Geneva, Switzerland, 2000
Canada Health (1999) Determination of tar, nicotine and carbon monoxide in mainstream tobacco smoke -official method. Ottawa, Health Canada
Šnyrychová I, Ayaydin F, Hideg É (2009) Detecting hydrogen peroxide in leaves in vivo—a comparison of methods. Physiol Plantarum 135(1):1–18
Mishin V, Gray JP, Heck DE, Laskin DL, Laskin JD (2010) Application of the Amplex red/horseradish peroxidase assay to measure hydrogen peroxide generation by recombinant microsomal enzymes. Free Radical Bio Med 48(11):1485–1491
Zhou M, Diwu Z, Panchuk-Voloshina N, Haugland RP (1997) A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253(2):162–168
Mohanty J, Jaffe J, Schulman E, Raible D (1997) Highly sensitive fluorescent micro-assay of hydrogen peroxide release from activated human leukocytes using a dihydroxyphenoxazine derivative. J Immunol Methods 202(2):133–141
Zhu M, Huang X, Liu L, Shen H (1997) Spectrophotometric determination of hydrogen peroxide by using the cleavage of Eriochrome black T in the presence of peroxidase. Talanta 44(8):1407–1412
Tang B, Wang Y, Sun Y, Xi Shen H (2002) Spectrofluorimetric determination of hydrogen peroxide with 2-hydroxy-1-naphthaldehyde salicyloylhydrazone (HNSH) as the substrate for horseradish peroxidase (HRP). Spectrochim Acta A 58(1):141–148
Li J, Dasgupta PK (2000) Measurement of atmospheric hydrogen peroxide and hydroxymethyl hydroperoxide with a diffusion scrubber and light emitting diode-liquid core waveguide-based fluorometry. Anal Chem 72(21):5338–5347
Zhang K, Mao L, Cai R (2000) Stopped-flow spectrophotometric determination of hydrogen peroxide with hemoglobin as catalyst. Talanta 51(1):179–186
Xu C, Zhang Z (2001) Fluorescence determination of hydrogen peroxide using hemoglobin as a mimetic enzyme of peroxidase. Anal Sci 17(12):1449–1451
Vaughan C, Stanfill SB, Polzin GM, Ashley DL, Watson CH (2008) Automated determination of seven phenolic compounds in mainstream tobacco smoke. Nicotine Tob Res 10(7):1261–1268
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This work was supported by the National Nature Science Foundation of China (No. 20875102).
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Liu, H., Sun, S., Zong, Y. et al. Analysis of hydrogen peroxide in cigarette smoke from selected Chinese cigarette brands under conventional and intense machine smoking conditions. Eur Food Res Technol 235, 1107–1115 (2012). https://doi.org/10.1007/s00217-012-1840-6
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DOI: https://doi.org/10.1007/s00217-012-1840-6