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Effects of suni-bug (Eurygaster spp.) damage on size distribution of durum wheat (Triticum durum L.) proteins

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Abstract

Wholemeal samples were obtained from five durum wheat cultivars at two different bug (Eurygaster spp.) damage levels (medium and high damage). The samples were incubated (60 and 120 min) and used in size exclusion high performance liquid chromatography (SE-HPLC) analyses. The results showed that the amount of larger polymeric protein (TP1) and smaller polymeric protein (TP2) obtained from total (sodium dodecyl sulfate soluble) proteins decreased significantly in the bug-damaged samples, while the amount of total larger monomeric proteins (TP3) increased. The polymeric/monomeric protein ratio of all cultivars decreased at 60 min of incubation with increasing damage level. For all cultivars, the ratio of unextractable polymeric protein (UPP%) significantly decreased at 60 min of incubation except cv. Diyarbakir. The results suggested that bug protease caused depolymerization and/or disaggregation of polymeric proteins to lower their average molecular size. The changes in protein structure as determined using SE-HPLC supported by the decreases in gluten content and gluten index values which decreased with suni-bug damage. Deteriorative effects of bug damage on durum wheat quality were found to be quite similar to those on bread wheats.

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Abbreviations

TP:

Total protein

EP:

Extractable protein

UP:

Unextractable protein

UPP:

Unextractable polymeric protein

SE-HPLC:

Size exclusion high performance liquid chromatography

SDS:

Sodium dodecyl sulfate

RPA:

Relative protease activity

HMW-GS:

High molecular weight glutenin subunit

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Acknowledgment

The project was supported by Turkish Scientific and Technical Research Council (Project No: TOVAG 104 O 479).

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Authors and Affiliations

  1. Food Engineering Department, Hacettepe University, 06800, Beytepe/Ankara, Turkey

    Burcu Olanca, Dilek Sivri Ozay & Hamit Koksel

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  1. Burcu Olanca
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  2. Dilek Sivri Ozay
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  3. Hamit Koksel
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Correspondence to Hamit Koksel.

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Olanca, B., Ozay, D.S. & Koksel, H. Effects of suni-bug (Eurygaster spp.) damage on size distribution of durum wheat (Triticum durum L.) proteins. Eur Food Res Technol 229, 813–820 (2009). https://doi.org/10.1007/s00217-009-1116-y

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  • DOI: https://doi.org/10.1007/s00217-009-1116-y

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