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CMOS image sensor for detection of interferon gamma protein interaction as a point-of-care approach

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Abstract

Complementary metal oxide semiconductor (CMOS)-based image sensors have received increased attention owing to the possibility of incorporating them into portable diagnostic devices. The present research examined the efficiency and sensitivity of a CMOS image sensor for the detection of antigen–antibody interactions involving interferon gamma protein without the aid of expensive instruments. The highest detection sensitivity of about 1 fg/ml primary antibody was achieved simply by a transmission mechanism. When photons are prevented from hitting the sensor surface, a reduction in digital output occurs in which the number of photons hitting the sensor surface is approximately proportional to the digital number. Nanoscale variation in substrate thickness after protein binding can be detected with high sensitivity by the CMOS image sensor. Therefore, this technique can be easily applied to smartphones or any clinical diagnostic devices for the detection of several biological entities, with high impact on the development of point-of-care applications.

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Acknowledgements

This work was partly supported by the ETRI Adventure Research Program (grant no. 10QC1110, Photosensitive Biosensor Array Chip for Mobile Devices) and was further supported by the Industrial Strategic Technology Development Program (10035197) funded by the Ministry of Knowledge Economy of Korea.

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Authors and Affiliations

  1. College of Bionanotechnology, Kyungwon University, San 65, Bokjeong-dong, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461 701, Republic of Korea

    Mohana Marimuthu, Karthikeyan Kandasamy & Sanghyo Kim

  2. BT Convergence Technology Research Department, Electronics and Telecommunications Research Institute (ETRI), Daejeon, 305-700, Republic of Korea

    Chang Geun Ahn & Gun Yong Sung

  3. Biomonitoring Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Republic of Korea

    Min-Gon Kim

Authors
  1. Mohana Marimuthu
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  2. Karthikeyan Kandasamy
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  3. Chang Geun Ahn
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  4. Gun Yong Sung
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  5. Min-Gon Kim
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  6. Sanghyo Kim
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Correspondence to Sanghyo Kim.

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Marimuthu, M., Kandasamy, K., Ahn, C.G. et al. CMOS image sensor for detection of interferon gamma protein interaction as a point-of-care approach. Anal Bioanal Chem 401, 1641–1649 (2011). https://doi.org/10.1007/s00216-011-5231-9

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  • DOI: https://doi.org/10.1007/s00216-011-5231-9

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