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Flavobacterium panacisoli sp. nov., isolated from soil of a ginseng field

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Abstract

A novel bacterial strain, designated DCY70T, was isolated from soil of a ginseng field in Republic of Korea and was characterized in order to determine its taxonomic position. The strain was Gram-reaction negative, yellow-pigmented, rod-shaped and catalase- and oxidase-positive. Based on the 16S rRNA gene sequence similarity, strain DCY70T was shown to belong to the genus Flavobacterium, most closely related to Flavobacterium oncorhynchi 631-08T (98.4 %), Flavobacterium plurextorum 1126-1H-08T (97.9 %), Flavobacterium chilense LM-09-FpT (97.9 %) and Flavobacterium chungangense CJT (97.7 %). The chemotaxonomic characteristics showed only menaquinone-6 (MK-6), iso-C15:0, iso-C15:0 3OH, iso-C17:0 3OH and summed feature 3 as major cellular fatty acids. Polar lipids were phosphatidylethanolamine (PE), two unidentified aminolipids, four unidentified polar lipids and one unidentified phospholipid. The DNA G+C content was 34.9 mol%. Based on the phylogenetic, phenotypic and genotypic data, a novel species, Flavobacterium panacisoli sp. nov., is proposed (=KCTC 32393T = JCM 19162T).

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Acknowledgments

This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (KIPET NO: 313038-03-2-SB010) and also supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant#: PJ0120342016), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 449-701, Republic of Korea

    Sun Young Jung, Yeon-Ju Kim, Van-An Hoang, Yan Jin, Ngoc-Lan Nguyen, Keun Huyn Oh & Deok-Chun Yang

  2. Graduate School of Biotechnology and Ginseng Bank, College of Life Science, Kyung Hee University, Yongin-si, Republic of Korea

    Deok-Chun Yang

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  1. Sun Young Jung
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  2. Yeon-Ju Kim
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  3. Van-An Hoang
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  4. Yan Jin
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  5. Ngoc-Lan Nguyen
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Corresponding authors

Correspondence to Yeon-Ju Kim or Deok-Chun Yang.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY70T is JX863366.

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Supplementary Fig. S1

Two-dimensional TLC of the polar lipid analysis. TCL of the total polar lipids of strain F. panacisoli DCY70T (I) and F.oncorhynchi CCUG 59446T (II), stained with 5 % ethanolicmolybdophosphoric acid for total polar lipids. Abbreviations: PE, phosphatidylethanolamine; AL1-2, unidentified aminolipids; L1-4, unidentified polarlipid; PL, unidentified phospholipid (DOCX 370 kb)

Supplementary Fig. S2

Transmission electron microscopy of the strain F. panacisoli DCY70T. Bar, 0.5 μm (TIFF 2416 kb)

Supplementary material 3 (DOCX 39 kb)

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Jung, S.Y., Kim, YJ., Hoang, VA. et al. Flavobacterium panacisoli sp. nov., isolated from soil of a ginseng field. Arch Microbiol 198, 645–651 (2016). https://doi.org/10.1007/s00203-016-1216-6

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  • DOI: https://doi.org/10.1007/s00203-016-1216-6

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