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Proteomic analysis of phytopathogenic fungus Botrytis cinerea as a potential tool for identifying pathogenicity factors, therapeutic targets and for basic research

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Abstract

Botrytis cinerea is a phytopathogenic fungus causing disease in a substantial number of economically important crops. In an attempt to identify putative fungal virulence factors, the two-dimensional gel electrophoresis (2-DE) protein profile from two B. cinerea strains differing in virulence and toxin production were compared. Protein extracts from fungal mycelium obtained by tissue homogenization were analyzed. The mycelial 2-DE protein profile revealed the existence of qualitative and quantitative differences between the analyzed strains. The lack of genomic data from B. cinerea required the use of peptide fragmentation data from MALDI-TOF/TOF and ESI ion trap for protein identification, resulting in the identification of 27 protein spots. A significant number of spots were identified as malate dehydrogenase (MDH) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The different expression patterns revealed by some of the identified proteins could be ascribed to differences in virulence between strains. Our results indicate that proteomic analysis are becoming an important tool to be used as a starting point for identifying new pathogenicity factors, therapeutic targets and for basic research on this plant pathogen in the postgenomic era.

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Abbreviations

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

MDH:

Malate dehydrogenase

PMF:

Peptide mass fingerprinting

2-DE:

Two-dimensional gel electrophoresis

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Acknowledgments

This research has been financed by the Spanish DGICYT (Project AGL2003-06480-C02-02). We thank Professor Isidro G. Collado (Department of Organic Chemistry, University of Cádiz, Spain) and the partners of AOSPLANT project for supplying the B. cinerea 1.11 strain.

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Authors and Affiliations

  1. Laboratory of Microbiology, Marine and Environmental Sciences Faculty, University of Cádiz, Pol. Río San Pedro, 11510, Puerto Real, Cádiz, Spain

    Francisco Javier Fernández-Acero, Inmaculada Vallejo, María Carbú, Carlos Garrido & Jesús Manuel Cantoral

  2. Agricultural and Plant Biochemistry Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Campus de Rabanales, Ed. Severo-Ochoa, 14071, Córdoba, Spain

    Inmaculada Jorge & Jesús Jorrin

  3. Proteomics Unit, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Ronda de Poniente, 28760, Tres Cantos, Madrid, Spain

    Enrique Calvo, Emilio Camafeita & Juan Antonio López

Authors
  1. Francisco Javier Fernández-Acero
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  2. Inmaculada Jorge
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  3. Enrique Calvo
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  4. Inmaculada Vallejo
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  5. María Carbú
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  6. Emilio Camafeita
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  7. Carlos Garrido
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  8. Juan Antonio López
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  9. Jesús Jorrin
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  10. Jesús Manuel Cantoral
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Correspondence to Jesús Manuel Cantoral.

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Fernández-Acero, F.J., Jorge, I., Calvo, E. et al. Proteomic analysis of phytopathogenic fungus Botrytis cinerea as a potential tool for identifying pathogenicity factors, therapeutic targets and for basic research. Arch Microbiol 187, 207–215 (2007). https://doi.org/10.1007/s00203-006-0188-3

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  • DOI: https://doi.org/10.1007/s00203-006-0188-3

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