Abstract
Objective
To elucidate the regulation, function of the chemokine CXC-motif ligand 12 (CXCL12) and its receptors (CXCR) 4 and 7 in prostate cancer tumor microenvironment.
Material
In-silico-analysis of expression in prostate cancer tissues. In-vitro comparison, testing of regulation in human prostate cancer cells LNCaP, DU145, and PC3.
Treatment
Dihydrotestosterone (DHT) treatments (0–10 nM) were for 0–48 h. The inflammatory agent Flagellin treatment (20 ng/ml) was for 2 h. Migration assays were performed for 24 h using 10 ng/ml CXCL12.
Methods
Real-time PCR, western analysis, and migration assays were used to determine mRNA, protein, and functional changes, respectively.
Results
Malignant prostate cancer tissues exhibit higher CXCR4/7 mRNA ratio, and higher CXCR7 mRNA levels were detected in the androgen-responsive LNCaP cells. Putative androgen-responsive elements were identified in CXCR4, 7 gene, and exposure to DHT, flagellin increased CXCR4 mRNA but decreased CXCR7 mRNA levels in LNCaP cells. Androgen receptor siRNA significantly attenuated the effects of DHT on CXCR4, 7 mRNA in LNCaP cells. However, DHT and flagellin only decrease CXCR7 protein and additively increased migration of LNCaP cells towards CXCL12.
Conclusions
Down regulation of CXCR7 protein by DHT and flagellin increased migration, supporting CXCR7 as decoy receptor counteracting CXCL12/CXCR4-mediated migration in prostate cancer cells.
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Acknowledgements
The authors thank Dr. Norberta Schoene for her comments and editorial assistance. This study was supported by US Department of Agriculture appropriated fund #8040–51530-057-00D
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Yu, L., Pham, Q., Yu, L.L. et al. Modulation of CXC-motif chemokine receptor 7, but not 4, expression is related to migration of the human prostate cancer cell LNCaP: regulation by androgen and inflammatory stimuli. Inflamm. Res. 69, 167–178 (2020). https://doi.org/10.1007/s00011-019-01305-0
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DOI: https://doi.org/10.1007/s00011-019-01305-0