Summary
A considerable number of studies have demonstrated the presence of NAD(P)-oxidoreductases in the plant and animal cell plasma membranes. Recently several attempts on the isolation and purification of these proteins have been presented. The results indicate the presence of distinct NAD(P)H-utilizing enzymes in the plasma membrane of several species. Proteins with molecular masses of 27 kDa, 31 kDa, 36–39 kDa, and 45 kDa have been identified. Little information is so far available on the presence and nature of the chromophores on these proteins. The electron donor and acceptor specificities of the purified enzymes seem to depend to some extent on the purification procedures used. Two interesting remarks became apparent when evaluating the literature available on this subject. First, although some plasma membrane NAD(P)H-oxidoreductase activity is transmembrane, none of the purified enzymes was reported to depend on the presence of polar lipids to reach full activity. Second, considerable amounts of enzyme activity were found in the non-solubilised membrane material and apparently resisted the solubilisation procedures. The nature of these activities has not yet been clarified. Clearly the amino acid sequencing and structural analysis of these proteins will reveal important new clues to the understanding of the plasma membrane electron transport in the near future.
Similar content being viewed by others
Abbreviations
- DQ:
-
duroquinone
- HCF:
-
hexacyanoferrate (III)
References
Asard H, Venken M, Caubergs RJ, Reijnders W, Oltmann FL, De Greef JA (1989)b-Type cytochromes in higher plant plasma membranes. Plant Physiol 90: 1077–1083
—, Horemans N, Caubergs RJ (1992) Transmembrane electron transport in ascorbate-loaded plasma membrane vesicles from higher plants involves ab-type cytochrome. FEBS Lett 306: 143–146
— — — (1995) Involvement of ascorbic acid and ab-type cytochrome in plant plasma membrane redox reactions. Protoplasma 184: 36–41
Askerlund P, Laurent P, Nakagawa H, Kader J-C (1991) NADH-ferricyanide reductase of leaf plasma membrane. Plant Physiol 95: 6–13
Bérczi A, Faulk WP (1992) Iron-reducing activity of plasma membranes. Biochem Int 28: 577–584
Brightman AO, Barr R, Crane FL, Morré DJ (1988) Auxin-stimulated NADH oxidase purified from plasma membrane of soybean. Plant Physiol 86: 1264–1269
—, Zhu XZ, Morré DJ (1991) Activation of plasma membrane NADH oxidase activity by products of phospholipase A. Plant Physiol 96: 1314–1320
Briskin DP (1990) The plasma membrane H+-ATPase of higher plant cells, biochemistry and transport function. Biochim Biophys Acta 1019: 95–109
Brüggeman W, Moog PR (1989) NADH-dependent Fe3+-EDTA and oxygen reduction by plasma membrane vesicles from barley roots. Physiol Plant 75: 245–254
Buckhout TJ, Luster DG (1991) Pyridine-nucleotide-dependent reductases of the plant plasma membrane. In: Crane FL, Morré DJ, Löw H (eds) Oxidoreduction at the plasma membrane: relation to growth and transport, vol 2, plants. CRC Press, Boca Raton, pp 61–84
Crane FL, Löw H, Clark MG (1985) Plasma membrane redox enzymes. In: Martonosi AN (ed) The enzymes of biological membranes. Plenum, New York, pp 465–509
—, Morré DI, Löw H (eds) (1988) Plasma membrane oxidoreductases in control of animal and plant growth. Plenum, New York (NATO ASI series, series A, vol 157)
—, Löw H, Morré DJ (1990) Historical perspective. In: Crane FL, Morré DJ, Löw H (eds) Oxidoreduction at the plasma membrane: relation to growth and transport, vol 1, animals. CRC Press, Boca Raton, pp 1–27
Dancis A, Klausner RD, Hinnebusch AG, Barriocanal JG (1990) Genetic evidence that ferric reductase is required for iron uptake inSaccharomyces cerevisiae. Mol Cell Biol 10: 2294–2301
—, Roman DG, Anderson GJ, Hinnebusch AG, Klausner RD (1992) Ferric reductase ofSaccharomyces cereviciae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci USA 89: 3869–3873
Guerrini F, Valenti V, Pupillo P (1987) Solubilization and purification of NAD(P)H dehydrogenase ofCucurbita microsomes. Plant Physiol 85: 828–834
Kaien A, Norling B, Appelkvist EL, Dallner G (1987) Ubiquinone biosynthesis by the microsomal fraction from rat liver. Biochim Biophys Acta 926: 70–78
Kasamo K, Nouchi I (1987) The role of phospholipids in plasma membrane ATPase activity inVigna radiata L. (mung bean) roots and hypocotyls. Plant Physiol 83: 323–328
Kim C, Crane FL, Becker GW, Morré DJ (1995) Purification of NADH-cytochromeb 5 reductase from rat liver plasma membranes. Protoplasma 184: 111–117
Lawrence J, Geilen C, Flesher G, Reutter W, Spicher G, Morré DJ (1995) The stimulation of NADH oxidase activity of rat liver plasma membranes by guanine nucleotides may involve both guanine nucleotide-binding proteins of the plasma membrane and responses not mediated by classic heterotrimeric G proteins. Protoplasma 184: 118–123
Löw H, Crane FL, Morré DJ, Sun IL (1990) Oxidoreductase enzymes in the plasma membrane. In: Crane FL, Morré DJ, Löw H (eds) Oxidoreduction at the plasma membrane: relation to growth and transport, vol 2, plants. CRC Press, Boca, pp 29–65
Luster DG, Buckhout TJ (1988) Characterization and partial purification of multiple electron transport activities in plasma membranes from maize (Zea mays) roots. Physiol Plant 73: 339–347
—, Buckhout TJ (1989) Purification and identification of a plasma membrane associated electron transport protein from maize (Zea mays) roots. Plant Physiol 91: 1014–1019
Lüthen H, Böttger M (1988) Hexachloroiridate IV as an electron acceptor for a plasmalemma redox system in maize roots. Plant Physiol 86: 1044–1047
Lüthje S, Böttger M (1989) Hexabromoiridate IV as an electron acceptor: comparison with hexachloroiridate IV and hexacyanoferrate III. Biochim Biophys Acta 997: 335–340
Marrè MT, Moroni A, Albergoni FG, Marré E (1988) Plasmalemma redox activity and H+-extrusion. I. Activation of the H+-pump by ferricyanide-induced potential depolarization and cytoplasm acidification. Plant Physiol 87: 25–29
Medina MA, de Castro IN (1995) Plasma membrane redox system in tumor cells. Protoplasma 184: 168–172
Møller IM, Crane FL (1990) Redox processes in the plasma membrane. In: Larson C, Møller IM (eds) The plant plasma membrane. Structure, function and molecular biology. Springer, Berlin Heidelberg New York Tokyo, pp 93–126
—, Askerlund P, Widell S (1991) Electron transport constituents in the plant plasma membrane. In: Crane FL, Morré DJ, Löw H (eds) Oxidoreduction at the plasma membrane: relation to growth and transport, vol 2, plants. CRC Press, Boca Raton, pp 35–59
—, Fredlund KM, Bérczi A (1995) The stereospecificity, purification, and characterization of an NADH-ferricyanide reductase spinach leaf plasma membrane. Protoplasma 184: 124–132
Morré DJ, Brightman AO (1991) NADH oxidase of plasma membranes. J Bioenerg Biomembr 23: 469–489
Palmgren MG, Sommarin M, Ulvskov P, Larsson C (1990) Effect of detergents on the H+-ATPase activity of inside-out and rightside-out plasma membrane vesicles. Biochim Biophys Acta 1021: 133–140
Raghavendra AS (1990) Blue light effects on stomata are mediated by the guard cell plasma membrane redox system distinct from the proton translocating ATPase. Plant Cell Environ 13: 105–110
Rubinstein B, Luster DG (1993) Plasma membrane redox activity: components and role in plant processes. Annu Rev Plant Physiol Plant Mol Biol 44: 131–155
Segal AW, Abo A (1993) The biochemical basis of the NADPH oxidase of phagocytes. Trends Biochem Sci 18: 43–47
Serrano A, Villalba JM, González-Reyes JA, Navas P, Córdoba F (1994) Two distinct NAD(P)H-dependent redox enzymes isolated from onion root plasma membranes. Biochem Mol Biol Int 32: 841–849
—, Córdoba F, González-Reyes JA, Navas P, Villalba JM (1994) Purification and characterization of two distinct NAD(P)H-dehydrogenases from onion (Allium cepa L.) root plasma membrane. Plant Physiol 106: 87–96
Serrano R (1989) Structure and function of the plasma membrane ATPase. Annu Rev Plant Physiol Plant Mol Biol 40: 61–90
Villalba JM, Canalejo A, Rodríguez-Aguilera JC, Burön MI, Morré DJ, Navas P (1993) NADH-ascorbate free radical and ferricyanide reductase activities represent different levels of plasma membrane electron transport. J Bioenerg Biomembr 25: 411–417
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bérczi, A., Asard, H. NAD(P)H-utilizing oxidoreductases of the plasma membrane An overview of presently purified proteins. Protoplasma 184, 140–144 (1995). https://doi.org/10.1007/BF01276911
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01276911