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Induction of chalcone synthase in cell suspension cultures of carrot (Daucus carota L. spp. sativus) by ultraviolet light: evidence for two different forms of chalcone synthase

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Abstract

Two cell lines of carrot (Daucus carota L. spp. sativus), grown as cell-suspension cultures in the dark, were irradiated with ultraviolet light (315–420 nm) 10 d after the onset of cultivation. Chalcone synthase (CHS) enzyme activity was induced in both cell lines. Anthocyanin synthesis was only stimulated in the anthocyanin-containing cell line DCb. Parallel to the increase in CHS activity there was an increase with time in the amount of one CHS form with an isoelectric point of 6.5 and a molecular weight of 40 kilodaltons (kDa) per subunit. Whereas the anthocyanin-free cell line DCs failed to accumulate anthocyanin, it did stimulate another CHS form with an isoelectric point at pH 5.5 and a molecular weight of 43 kDa per subunit. Both enzyme activities could be separated by isoelectric focusing and stabilized using sodium hydrosulfite as an oxidation protectant. In carrot plants, CHS was restricted to the dark purple petals of the inflorescence (40 kDa) and to the leaves (43 kDa).

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Abbreviations

BSA:

bovine serum albumin

CHS:

chalcone synthase

IEF:

isoelectric focusing

kDa:

kilodaltons

KPi :

potassium phosphate buffer

PAL:

phenylalanine ammonialyase

pI:

isoelectric point

UV:

ultraviolet

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  1. Allgemeine Botanik und Pflanzenphysiologie, Botanisches Institut der Universität, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany

    Johannes Gleitz & Hanns Ulrich Seitz

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  1. Johannes Gleitz
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  2. Hanns Ulrich Seitz
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Gleitz, J., Seitz, H.U. Induction of chalcone synthase in cell suspension cultures of carrot (Daucus carota L. spp. sativus) by ultraviolet light: evidence for two different forms of chalcone synthase. Planta 179, 323–330 (1989). https://doi.org/10.1007/BF00391077

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  • DOI: https://doi.org/10.1007/BF00391077

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