Summary
We have studied the influence of several parameters (glutaraldehyde concentration, pH and contact time) on the activation of an amine silica with glutaraldehyde. In order to take interactions between parameters into consideration, we have used the Response Surface Methodology which allows us to obtain an equation (not a single estimation). The optimal conditions for glutaraldehyde activation of amine Spherosil beads were as follow: the porous silica was activated for 1 h at 25°C with an 8% (V/V) solution of glutaraldehyde at pH 6.4 these conditions were the same with RNA or CMP used as substrates.
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Marty, JL. Application of response surface methodology to optimization of glutaraldehyde activation of a support for enzyme immobilization. Appl Microbiol Biotechnol 22, 88–91 (1985). https://doi.org/10.1007/BF00250025
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DOI: https://doi.org/10.1007/BF00250025