AAS and GC-MS Analysis of Phytocomponents in the Leaf, Stem and Root of Azadirachta indica A. Juss (Dongoyaro)
Momoh Johnson Oshiobugie *
Department of Science Laboratory Technology (Biochemistry Unit), School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos, Nigeria.
Adeniyi Michael Olaniyi
Department of Mathematics and Statistics, School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos, Nigeria.
Aderele Oluwaseun Raphael
Department of Mathematics and Statistics, School of Pure and Applied Sciences, Lagos State Polytechnic, Ikorodu, Lagos, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Objective: To determine the mineral component using AAS and screen for the presence of bioactive phytoconstituents in hexane extract of Azadirachta indica A. Juss leaf, stem and root using the GC-MS technique.
Methods: Mineral analysis was carried out using AAS. The leaf, stem and root hexane extracts of A. indica were prepared by standard procedure and concentrated at 40ºC using hot air oven. The concentrated hexane extracts were subjected to phytochemical analysis using GC-MS.
Results: The result of mineral analysis shows that Azadirachta indica A. Juss leaf, stem and root contain potassium, iron, copper, calcium, magnesium and sodium. The GC-MS analysis of the neem leaf, stem and root extract revealed the existence of the GC-MS chromatogram of twenty three peaks present. Ten chemical constituents were identified in the leaf of A. indica, six were found in the stem while seven were identified in the root of the plant by Gas Chromatogram Mass spectrometry (GC-MS) analysis.
Conclusion: The result of the analysis showed that the plant contains important minerals and many pharmacologically important bioactive compounds. The presence of various bioactive compounds justifies the uses of Neem for various traditional medicines.
Keywords: AAS, Azadirachta indica A. Juss, GC-MS analysis, biological activities.