Evaluation of Antioxidant, Antimicrobial, Antityrosinase and Cytotoxic Potentials of Isatis cappadocica subsp. alyssifoli as a Potent Pharmaceutical Resource
Meltem Güner
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Atila Taner Kalaycioglu
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Seyda Kanbolat
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Nuriye Korkmaz
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Rezzan Aliyazicioglu *
Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Mahmoud Abudayyak
Department of Pharmaceutic Toxicology, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
Ali Kandemir
Department of Biology, Faculty of Science, Erzincan University, 24000, Erzincan, Turkey.
Sengul Alpay Karaoglu
Department of Biology, Faculty of Arts and Sciences, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Ufuk Ozgen
Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
*Author to whom correspondence should be addressed.
Abstract
Isatis species, which are endemic across most of Turkey, exhibit antibacterial, anticancer and antiviral effects. The aim of this study was to determine of antioxidant, antimicrobial, tyrosinase inhibitor, cytotoxic activities, and phenolic profile of polar extracts of Isatis cappadocica Desv. We analyzed the antioxidant properties of extracts using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging activity. The phenolic composition of methanolic extract of I. cappadocica was analyzed by reverse phase high performance liquid chromatography (RP-HPLC). Extracts’ antimicrobial properties were evaluated based on the agar well diffusion technique. Tyrosinase inhibitory activity was measured colorimetrically. Methanolic extract yielded better FRAP and CUPRAC results and aqueous extract yielded better DPPH activity. Benzoic acid, and sinapic acid were detected as major phenolic compounds. Methanolic extract was particularly effective against all the bacteria investigated, apart from Yersinia pseudotuberculosis. Methanol extract was exhibited tyrosinase inhibitory activity. The methanol extract has caused to death of cells by dosage in the high concentrations cytotoxic activity on the PC-3 and 3T3 cell lines. The results showed that I. cappadocica could be used as a natural source in the food, cosmetic, and drug industries due to their rich antioxidant, antimicrobial, cytotoxic and tyrosinase inhibitor activities.
Keywords: Isatis cappadocica, antioxidant, antimicrobial, antityrosinase, cytotoxicity