Multiple Shoot Regeneration from Nodal Explants of Gynura procumbens (Lour.) Merr
Z. Alizah *
Biotechnology Research Centre Malaysian Agriculture Research and Development Institute Serdang, P.O. Box 12301, Kuala Lumpur, Malaysia.
Y. Nurulaishah
Biotechnology Research Centre Malaysian Agriculture Research and Development Institute Serdang, P.O. Box 12301, Kuala Lumpur, Malaysia.
*Author to whom correspondence should be addressed.
Abstract
Gynura procumbens (Lour.) Merr. is an important medicinal plants and used to treat various ailments such as diabetes, hypertension and urinary tract infection. Multiple shoot formation was induced from nodal segment of G. procumbens cultured on Murashige and Skoog (MS) medium containing various concentrations 6-benzylaminopurine (BAP). The MS medium with 3 mg/l BAP gave the highest number of shoots per explant. All the micro-shoots produced normal roots within two weeks of culture on basic MS medium without any plant growth regulators. Regenerated plants were aclimatised before transferred to field condition and showed similar morphology to field grown plants.
Keywords: Gynura procumbens, tissue culture, nodal segment, shoot regeneration