The structural regeneration of biological tissue is imperative for recovery of the organ function during tissue healing process. The mechanical and biological function of bone tissue is governed by the oriented microstructure of collagen/apatite matrix. Control of unidirectional cell alignment triggers the subsequent oriented bone matrix organization. However, the development of biomedical devices equipped with ideal design for microstructural recovery of bone tissue is not yet fully achieved. Mesenchymal stem cells （MSCs） play important roles in bone tissue regeneration, which have been considered as a promising therapeutic target easily isolatable form the patients. Here, we propose additive manufacturing（AM）technology as a powerful tool for control of the differentiation fate and the function of mesenchymal stem cells. MSCs aligned along the grooved structure fabricated by selective laser melting. Moreover, the aligned MSCs showed upregulated expression of BGLAP, an important osteogenic differentiation marker gene. The results indicate the technological advances of AM process, which realize the functional bone tissue regeneration from MSCs.