Chemical composition, antioxidant and antitumor activities of sub-fractions of wild and cultivated Pleurotus ferulae ethanol extracts

Pleurotus ferulae is an edible and medicinal mushroom with various bioactivities. Here, the ethanol extracts of wild and cultivated P. ferulae (PFEE-W and PFEE-C) and their subfractions including petroleum ether (Pe-W/Pe-C), ethyl acetate (Ea-W/Ea-C) and n-butanol (Ba-W/Ba-C) were prepared to evaluate their antioxidant and antitumor activities. Both PFEE-W and PFEE-C show the antioxidant activity and PFEE-W is stronger than PFEE-C. The antioxidant activities of their subfractions are in the following order: Ea > Ba > Pe. Moreover, PFEE-W and PFEE-C significantly inhibit the proliferation of murine melanoma B16 cells, human esophageal cancer Eca-109 cells, human gastric cancer BGC823 cells and human cervical cancer HeLa cells through induction of apoptosis, which partially mediated by reactive oxygen species. The antitumor activities of their subfractions are in the following order: Ea ≥ Pe > Ba. Pe-W shows higher antitumor activity compared with Pe-C, which might be correlated with the difference of their components identified by gas chromatography-mass spectrometry. These results suggest that both wild and cultivated P. ferulae have antioxidant and antitumor activities, and cultivated P. ferulae could be used to replace wild one in some functions.


INTRODUCTION
Currently, cancer is the second leading cause of death in the world. In 2015, there were 17.5 million cancer cases and 8.7 million deaths globally, which included around 4.3 million cancer cases and 2.8 million deaths in China (Chen et al., 2016;Global Burden of Disease Cancer Collaboration et al., 2017). Different strategies including surgery, chemotherapy, radiotherapy, hormone therapy, targeted therapy or their combinations have been developed to treat cancer (Chang et al., 2015;Eberhardt et al., 2015;Le Tourneau et al., 2015;Perez et al., 2014).
Medicinal fungi have various biological functions and become a hot spot in functional food and medical research. Pleurotus ferulae is an edible and medicinal The optical density (OD) was measured at 490 nm using a 96-well microplate reader (Bio-Rad Laboratories, Hercules, CA, USA). The polysaccharide content was calculated according to the standard curve made by the standard of glucose.

Determination of polyphenol content
The polyphenol contents of PFEE-W/C or subfractions were determined by ferrous tartrate method (Yu et al., 2007). The OD was measured at 540 nm using a 96-well microplate reader. The polyphenol content was calculated according to the standard curve made by the standard of gallic acid.

Determination of total flavonoid content
The flavonoid contents of PFEE-W/C or subfractions were detected according to previous description (Swamy, Sinniah & Akhtar, 2015). The OD was detected at 517 nm using a 96-well microplate reader. The content of flavonoids was calculated according to the standard curve obtained by the standard of rutin.

Diphenylpicrylhydrazyl radical scavenging assay
Diphenylpicrylhydrazyl (DPPH) radical scavenging assay was carried out according to previously described (Molyneux, 2004). Briefly, PFEE-W/C or subfractions were dissolved in ethanol and mixed with 0.1 mM DPPH. The ascorbic acid was used as positive control and the mixture of ethanol and DPPH was used as negative control. The OD was measured at 517 nm using a microplate reader. Tests were carried out in triplicate. The percentage of DPPH radical scavenging was calculated according to the equation: A s is the OD of the sample mixed with DPPH, A c is the OD of sample without DPPH and A b is the OD of the ethanol mixed with DPPH.

Reducing power
The reducing activities of PFEE-W/C or subfractions were carried out according to previous description (Yildirim, Mavi & Kara, 2001). The ascorbic acid was used as positive control. The OD was measured at 700 nm.

MTT assay
MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] (Sigma-Aldrich, St. Louis, MO, USA) assay was used to evaluate the effects of PFEE-W/C and subfractions on the proliferation of tumor cells. The tumor cells at the logarithmic phase of growth were seeded in 96-well plates at a density of 5 Â 10 3 cells/well and cultured overnight, then treated with PFEE-W/C or subfractions at various concentrations (0, 100, 200, 400, 600 mg/ml) or 0.6% DMSO (equal to that in 600 mg/ml) for 24 h. Cisplatin was used as a positive control. After centrifugation at 1,200 rpm for 7 min, supernatant was discarded and 100 ml of MTT solution (0.5 mg/ml in PBS) was added into each well and incubated at 37 C for 4 h. The formed formazan crystals were dissolved in 150 ml DMSO. The OD490 values were measured by a 96-well microplate reader. The relative cell viability was calculated as the followed formula: Cell viability (%) = (OD treated /OD untreated ) Â 100%.

Hoechst 33342 staining
After treatment with 400 mg/ml PFEE-W/C and subfractions or 0.4% DMSO for 24 h, HeLa cells were washed with PBS and fixed with 4% ice-cold paraformaldehyde at 4 C for 10 min. Then cells were stained with Hoechst 33342 (Beyotime, Shanghai, China) at 4 C for 10 min in the dark after washing with PBS. Samples were observed by inverted fluorescence microscope (Nikon Eclipse Ti-E, Tokyo, Japan).

Measurement of reactive oxygen species
Intracellular reactive oxygen species (ROS) production was measured using DCFH-DA probes. HeLa cells were treated with 400 mg/ml Ea-W/Ea-C or 0.4% DMSO for 24 h. After treatment, all cells were harvested and stained by 10 mM of fluorescent probe DCFH-DA (Beyotime, Shanghai, China) for 20 min at 37 C. After washing three times with PBS, the fluorescence intensity in cells was determined by flow cytometry.

Gas chromatography-mass spectrometry
The samples were prepared according to the following procedure. Briefly, 50 mg Pe-W/Pe-C were mixed with one ml methanol and 50 ml concentrated sulfuric acid, followed by reflux for 4 h. After cooling, saturated NaCl solution was add to terminate the reaction. Then, the solution was extracted with two ml n-hexane, and the upper layer was collected for GC-MS analysis. The methyl derivatives of fatty acids were identified by a 7890A-5975C gas chromatograph equipped with a PE-5MS capillary column (30 m Â 0.25 mm Â 0.25 mm). Helium (flow rate, 1.0 ml/min) was used as the carrier gas. The column temperature was maintained initially at 100 C for 1 min, followed by increasing to 200 C at a rate of 3 C/min, from 200 to 250 C at a rate of 5 C/min, and then kept at 250 C for 30 min. The electron impact (EI) energy was 70 eV and the ion source temperature was 230 C. Split ratio was 50:1. EI mass spectra were recorded in the 33-450 amu range at 1 s intervals. The library search was carried out using NIST GC-MS libraries.

Statistical analysis
We expressed all data as mean ± standard error of the mean. Statistical analysis was performed by GraphPad Prism 5.0, and conducted with one-way analysis of variance. The two-tailed paired t-test was used to compare PFEE-W and PFEE-C and their subfractions. p < 0.05 was considered to be statistically significant.

RESULTS
The polysaccharide, polyphenol and flavonoid contents of PFEE-W and PFEE-C and subfractions PFEE-W/C and subfractions were prepared to analyze the contents of polysaccharides, polyphenols and flavonoids. Compared with PFEE-C, PFEE-W contained higher concentration of flavonoids (1.202 vs 1.04 mg/ml), lower concentration of polysaccharides (38.46 vs 54.87 mg/ml) and similar concentration of polyphenols (Table 1). The polysaccharide and polyphenol contents in Pe-W are lower than that in Pe-C, while the flavonoid contents in Pe-W and Pe-C are similar. Ea-W and Ea-C contain similar polysaccharide contents but Ea-W contains higher concents of polyphenols and flavonoids than that of Ea-C. Same as Ea-W/Ea-C, Ba-W and Ba-C also contain similar polysaccharide contents, while Ba-W contains higher contents of polyphenols and flavonoids than that of Ba-C.

Antioxidant activities of PFEE-W/C and subfractions
The antioxidant activities of PFEE-W/C and subfractions were measured by DPPH radical scavenging assay. As shown in Figs. 1A-1D, all fractions showed remarkable radical scavenging activities in a dose-dependent manner. Generally, the radical scavenging activities of fractions from wild P. ferulae are higher than that of cultivated P. ferulae. The scavenging activity of ethyl acetate fraction was higher than those of petroleum ether and n-butanol fractions both in wild and cultivated P. ferulae. However, the scavenging activities of PFEE-W/C and subfractions were lower than that of V c , a positive control, which reached the 100% scavenging rate at one mg/ml. The antioxidant activities of all fractions were further evaluated by reducing power. Similarly, all fractions showed reducing power in a dose-dependent manner, and V c showed higher reducing power than that of PFEE-W/C and subfractions (Figs. 1E-1H). The reducing power of fractions from wild P. ferulae is slightly higher than that of cultivated P. ferulae. In addition, the reducing power of fractions from both wild and cultivated P. ferulae followed the order: PFEE > Ea ! Ba > Pe. These results indicate that PFEE-W/C and subfractions have antioxidant activities.

PFEE-W/C and subfractions suppress the growth of tumor cells
The antitumor effect of PFEE-W/C and subfractions was detected and compared by MTT assay. B16, Eca-109, BGC823 and HeLa cells were treated with different concentrations of PFEE-W/C and subfractions for 24 h. As shown in Fig. 2, PFEE-W/C and subfractions significantly reduced the viability of B16, Eca-109 and HeLa cells in a dose-dependent manner except BGC823 cells that were not sensitive. PFEE-W and PFEE-C showed similar antitumor activities against B16 and Eca-109 cells but the antitumor activity of PFEE-W was higher than that of PFEE-C for HeLa cells. Furthermore, subfractions from wild and cultivated P. ferulae showed different antitumor activities. For Eca-109 and HeLa cells, Pe-W and Ea-W exhibited higher antitumor activities than that of Pe-C and Ea-C, respectively. The antitumor activities of subfractions from both wild and cultivated P. ferulae followed the order: Ea ! Pe > Ba. These results indicate that PFEE-W/C and subfractions have antitumor activities.
We also detected the cytotoxicity of PFEE-W/C and subfractions on mouse liver NCTC1469 cells. Generally, the low concentrations of PFEE-W/C and subfractions showed no cytotoxicity, while the high concentrations of PFEE-W/C and subfractions significantly reduced the viability of NCTC1469 cells (Fig. S1). However, the cytotoxicity of PFEE-W/C and subfractions on NCTC1469 cells was much lower than that on tumor cells, suggesting that PFEE-W/C and subfractions have minor side effect on normal cells.

Chemical composition of fatty acids of Pe-W and Pe-C
In order to find out the components caused the different antitumor activities, Pe-W and Pe-C were selected to do GC-MS due to their much difference of antitumor activities. The fatty acid compositions of the two subfractions were identified by GC-MS (Fig. S2). A total of 21 components were identified in Pe-W (Table 2), which mainly contained methyl linoleate (60.95%), hexadecanoic acid methyl ester (10.44%), 11-octadecenoic acid methyl ester (6.95%) and hexadecanoic acid (4.1%). A total of 25 components were identified in Pe-C (Table 2), which mainly contained methyl linoleate (58.25%), methyl oleate (15.23%), hexadecanoic acid methyl ester (14.32%) and myo-inositol hexaacetate (6.15%). Compared the major constituents in Pe-W and Pe-C, the contents of methyl linoleate and hexadecanoic acid methyl ester are similar but the contents of 11-octadecenoic acid methyl ester and methyl stearate are different. Except for the quantitative differences of some components, the qualitative differences were also found between Pe-W and Pe-C. Some components including hexadecanoic acid, 12-methyl-E, E-2,13-octadecadien-1-ol, desmosterol, ergosterol were found in Pe-W but not in Pe-C. Other components including methyl oleate and myo-inositol hexaacetate were found

PFEE-W/C and subfractions induce apoptosis of HeLa cells
To investigate whether PFEE-W/C and subfractions can induce apoptosis of tumor cells, HeLa cells were selected and treated with 400 mg/ml of PFEE-W/C, Pe-W/C and Ea-W/C for 24 h. After Annexin V-FITC and PI staining, cell apoptosis was analyzed by flow cytometry. Compared with untreated group, the frequencies of apoptotic and necrotic HeLa cells were significantly increased after PFEE-W/C, Pe-W/C and Ea-W/C treatment, but these extracts mainly induced apoptosis (>60%) compared with necrosis (<20%) (Figs. 3A-3C). PFEE-W and PFEE-C have similar activities in the induction of apoptosis and necrosis. Pe-W and Ea-W show higher activities than Pe-C and Ea-C, respectively, in the induction of apoptosis and necrosis. Furthermore, Hoechst 33342 staining was used to observe the morphology of nuclei of HeLa cells upon PFEE-W/C and subfractions treatment. As shown in Figs. 3D and 3E, the nuclei of untreated cells were homogeneously stained, but the nuclei of PFEE-W/C and subfractions treated cells showed condensed and fragmented. These results indicated that PFEE-W/C and subfractions induced apoptosis of HeLa cells.

ROS partially mediates the induction of apoptosis by Ea-W and Ea-C
Reactive oxygen species plays an important role in the induction of apoptosis (Redza-Dutordoir & Averill-Bates, 2016). Due to the high antitumor activity, Ea-W and Ea-C were selected to investigate the role of ROS in the induction of apoptosis. After treatment for 24 h, Hela cells were stained with the specific fluorescence probe DCFH-DA to detect intracellular ROS levels. As shown in Figs. 4A and 4B, ROS production showed dynamically changes upon Ea-W and Ea-C treatment. ROS production was significantly increased at 2 h and gradually decreased from 8 to 24 h, then dramatically increased at 48 h. Next, NAC, a ROS scavenger, was used to test the role of ROS in the induction of apoptosis. As shown in Figs. 4C-4E, NAC pretreatment partially inhibited the apoptosis of HeLa cells induced by Ea-W and Ea-C, suggesting that ROS produced during the first 2 h promoted the induction of apoptosis.

DISCUSSION
Several studies including ours have been reported that P. ferulae contains a number of active components such as polysaccharides, flavonoids and polyphenols, and exhibits antioxidant, antitumor, antihyperglycemic and immunoregulatory activities (Choi et al., 2004(Choi et al., , 2016Alam et al., 2012, Li et al., 2015aWang et al., 2014). However, cultivated P. ferulae has been used in almost studies including ours due to the resource of wild P. ferulae is scarce. It is still elusive whether wild and cultivated P. ferulae have similar or different activities of antioxidation and antitumor. Here, the ethanol extracts of wild and cultivated P. ferulae and their subfractions were prepared to compare their components and antioxidant and antitumor activities. The results show that PFEE-C Compared with PFEE-C and its subfractions, PFEE-W and its some subfractions showed higher antioxidant and antitumor activities to a certain extent, which might be correlated with the differences of their components. Due to the much difference of antitumor activities between Pe-W and Pe-C, the components of Pe-W and Pe-C were identified by GC-MS. We found that the predominant constituents in Pe-W and Pe-C were methyl linoleate (60.95% vs 58.25%) and hexadecanoic acid methyl ester (10.44% vs 14.32%). Linoleic acid and its methyl esters have many beneficial effects in the prevention of atherosclerosis, cancer, hypertension and improvement of immune function (Whelan, 2008;Bhattacharya et al., 2006). It has been reported that linoleic acid or conjugated linoleic acid can inhibit the proliferation of hybridoma cells or human breast cancer MCF74 cells through induction of apoptosis or activation of p53, respectively (Albright et al., 2005;Kisztelinski et al., 2006). These studies suggest that methyl linoleate may be associated with the antitumor effects of Pe-W and Pe-C. However, other two major constituents in Pe-W and Pe-C are much different, 11-octadecenoic acid methyl ester (6.95% vs 0.73%) and methyl oleate (0% vs 15.23%). Moreover, seven components including hexadecanoic acid, hexadecanoic acid, 2-hydroxy-, methyl ester, 12-methyl-E,E-2,13-octadecadien-1-ol, Z,E-3,13-Octadecadien-1-ol, (22Z)-Cholesta-5,7,22 -trien-3-ol, desmosterol, ergosterol were found in Pe-W but not in Pe-C. Some of them may have antitumor activity, such as ergosterol, which can reduce the breast cancer cell viability through induction of apoptosis and up-regulation of Foxo3 expression (Li et al., 2015b). The difference of components might cause the different antitumor activities of Pe-W and Pe-C. Therefore, it is worth further isolating and identifying the constituents that cause the different antitumor activities between wild and cultivated P. ferulae.
Our previous study showed that PFEE-C could inhibit the growth of tumor cells through induction of apoptosis . Similarly, PFEE-W/C and their subfractions significantly induced apoptosis of HeLa cells. Furthermore, ROS production induced by Ea-W and Ea-C partially caused apoptosis. However, ROS production showed dynamically changes upon Ea-W and Ea-C treatment, which might be correlated with their antioxidant activities. The possible reason is that some components in Ea-W and Ea-C quickly induced ROS production during the first 2 h and gradually scavenged by other components in Ea-W and Ea-C from 8 to 24 h. Finally, ROS production reerupted at 48 h due to the progress of apoptosis in cells.

CONCLUSION
These results indicated that the different extracts of wild and cultivated P. ferulae possessed antioxidant and antitumor activities. Although there are some differences in antioxidant and antitumor activities, cultivated P. ferulae could replace the wild one to a great extent. P. ferulae could be used to develop functional food with antioxidant and antitumor activities.

ADDITIONAL INFORMATION AND DECLARATIONS Funding
This work was supported by the "13th Five-Year" Plan for Key Discipline Biology Bidding Project (17SDKD0202), Xinjiang Normal University to Jinyu Li and the Chinese National Natural Science Foundation Grant (31460241) and the High Level Talent Introduction Project of Xinjiang Uygur Autonomous Region to Jinyao Li. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Grant Disclosures
The following grant information was disclosed by the authors: "13th Five-Year" Plan for Key Discipline Biology Bidding Project: 17SDKD0202. Xinjiang Normal University to Jinyu Li and the Chinese National Natural Science Foundation: 31460241. High Level Talent Introduction Project of Xinjiang Uygur Autonomous Region to Jinyao Li.