Distribution of deep-water corals, sponges, and demersal fisheries landings in Southern California, USA: implications for conservation priorities

Deep-sea corals in Southern California are diverse and abundant but subject to multiple stressors, including bottom-contact fisheries using mobile and fixed gear. There is a need for more information on the distribution of these taxa in relation to the distribution of demersal fishing effort, and the distribution of marine protected areas, in order to improve spatial planning. There are many marine managed areas in Southern California, including essential fish habitat (EFH) areas, conservation areas, and a national marine sanctuary, but specific areas of overlap between bottom fishing and benthic epifauna are poorly known. Groundfish surveys were conducted by the National Marine Fisheries Service using a remotely operated vehicle throughout Southern California between 2003 and 2011 to document abundance and distribution of deep-water rockfish and flatfish to a depth of 500 m. Corals and sponges were also common in these images, providing an opportunity to examine these communities. Analyses of 34,792 still images revealed abundance and diversity of coral and sponge taxa, as well as frequency of fishing debris. The occurrence data were overlaid in a geographic information system with landings data for deep-water (>50 m) demersal fisheries to identify areas of spatial overlap. Corals or sponges were observed in 23% of images. A total of 15 coral genera and six sponge morphotypes were identified. A total of 70 species codes were targeted by deep-water demersal fisheries operating below 50 m for years 2007–2011. A novel priority-setting algorithm was developed to identify areas of high richness, abundance, and fishing intensity (RAFi). Several highly-ranked areas were already protected as EFH (Footprint, Piggy Bank). Other highly-ranked sites (West Catalina Island, San Clemente Island, 9-Mile Bank, Santa Rosa Flats) were encompassed by transient gear restrictions, such as Rockfish conservation areas, but are now recommended for permanent protection by the Pacific Fishery Management Council.


INTRODUCTION
Deep-water (>50 m) azooxanthellate coral and sponge communities are widespread in the Northeast Pacific, from the Bering Sea west to the Hawaiian Islands and south to the tip of Baja California (Verrill, 1870;Ostarello, 1973). In this region, deep-water coral species are reported from 50 to 3,880 m, but are most abundant between 50 and 1,000 m . These deep-water communities provide habitat for fishes and invertebrates (Husebø et al., 2002;Ross & Quattrini, 2007;Love et al., 2007;Stone, 2006) and they are considered important components of deep-sea benthic ecosystems that provide areas for juvenile development and shelter from predation (Baillon et al., 2012).
The complex bathymetry of the seafloor and cold, highly productive upwelling oceanography of the California Current Ecosystem are conducive to deep-water coral and sponge growth within the Southern California Bight (SCB; Point Conception, CA, to the U.S.-Mexico Border). Habitat suitability models based on topographic and environmental data and a growing National Database of Deep-Sea Coral occurrences (Hourigan et al., 2015) suggest high likelihood for several families of corals in the waters surrounding the islands of San Nicolas, Santa Catalina, and San Clemente (Huff et al., 2013;Guinotte & Davies, 2014).
Due to the challenges and high costs of surveying remote deep-sea ecosystems, much remains unknown about the distribution and abundance of corals and sponges in these areas. Much of what is known about deep-sea corals and sponges in the SCB is based on museum records  and published observations from submersible expeditions (Tissot et al., 2006;Love et al., 2007;Yoklavich et al., 2011). Other records come from telepresence expeditions using ROVs and incidental bycatch in commercial fisheries. These records are compiled through NOAA's National Database of Deep-Sea Corals (NOAA, 2017), but to date there has been no synthesis of these occurrences in relation to fisheries landings in the SCB, such as the analysis provided here.
The limited information that is available on these sessile organisms suggests that they are potentially vulnerable to disturbance from bottom-contact fishing gear. Deep-sea corals and sponges are slow-growing and often live hundreds of years (Roark et al., 2005;Andrews et al., 2002). These corals and sponges also co-occur with many commercially important fish species that also inhabit deep, rocky substrates, and therefore may be negatively impacted by bottom-contact fixed gear (e.g., set nets, longlines, and traps) and mobile gear (e.g., trawls) in these areas Whitmire & Clarke, 2007;Parker & Bowden, 2010;Shester & Micheli, 2011;Rooper et al., 2017). Closed areas have been established that provide corals and sponges with permanent (e.g., essential fish habitat (EFH), marine reserves) or temporary (e.g., fishery conservation areas) de facto protections from some bottom-contact fishing activities (California Department of Fish and Wildlife, 2016), but these temporary areas may be reopened to bottom fishing once target fish populations rebuild (Pacific Fishery Management Council, 2006).
Fishery management plans for groundfish describe EFH as the waters and substrate needed for breeding, feeding, and growth to maturity (Pacific Fishery Management Council, Amendment 19, 2006). EFH areas that are important to ecological function, rare, or sensitive to environmental degradation and development are identified as "habitat areas of particular concern" (HAPC). Estuaries, kelp beds, seagrass, and rocky reefs are types of HAPC for groundfish. Rocky reefs are considered the least abundant but most important HAPC (Pacific Fishery Management Council, Amendment 19, 2006) and are often invoked as the best habitat for corals and sponges (Tissot et al., 2006;Whitmire & Clarke, 2007;Yoklavich et al., 2011). There is a legal mandate in the Magnuson-Stevens Fishery Conservation and Management Act (2006) to minimize adverse impacts to EFH as well as discretionary authority in the Act to protect deep-sea coral ecosystems.
The objectives of this study were to examine the spatial relationships between deep-water bottom-contact fisheries and deep-sea corals and sponges in the SCB. We compared data on the abundance and diversity of deep-sea corals and sponges from ROV surveys with the frequency of observed derelict fishing gear and fishery landings (2007)(2008)(2009)(2010)(2011) from adjacent areas (Perry et al., 2010;California Department of Fish and Wildlife, 2011). This was done in order to examine areas of potential overlap across the region and within particular depth ranges. The period from 2007 to 2011 is a temporal frame of reference for fisheries managers, since several protected areas were established in 2006. California Department of Fish and Wildlife (CDFW) catch block data has been used to track the distribution of fisheries along the California coast since 1935 (Miller et al., 2014).
Benthic images were used to estimate diversity and abundance of corals and sponges at the mesoscale of a feature, or named place, referred to as a site. Many ROV dives occurred at each site, and many images were taken on each dive. The images captured information on number, size, and identity of organisms and derelict fishing gear, as well as their frequency of occurrence. These data were combined with regional fishery landings to develop a novel conservation index used to identify areas where interactions between fisheries and sensitive habitats are likely to have occurred, and where there is high diversity and abundance, but little evidence of fishing. This study does not address indirect impacts from fishing; however, the RAFi index is consistent with the goals of HAPC to identify habitats that are sensitive or rare, in terms of abundance and diversity; or stressed, in terms of potential fishing impacts.

Survey area and image collection
High-resolution still images were collected during 352 ROV transects conducted during surveys of abalone Haliotis sp. (Stierhoff, Neuman & Butler, 2012) and rockfishes Sebastes and Sebastolobus spp. (Demer, 2012) in SCB between the depths of 27 and 579 m during the period between November 5th, 2003 and December 7th, 2011 ( Fig. 1). Transects were haphazard, focused on predominantly rocky areas with moderate relief, generally working from deep-water to shallow water over an average distance between one and two km per dive. During these surveys, still photographs were collected periodically to aid identification of demersal fishes and abalone; however, many corals and sponges were also documented. Still photographs of non-target corals and sponges were collected opportunistically. All unique images where the bottom was visible were used to describe the distribution of corals and sponges in the study area.
A total of 34,792 still images were collected at 32 banks and ridges throughout the SCB (Fig. 1). Photos were collected using a three megapixel digital still camera Deep Ocean Engineering, Inc., San Jose, CA, USA). Two pairs of parallel lasers spaced at 20 and 61 cm apart provided a size reference. A conductivity, temperature, and depth (CTD) sensor (Citadel CTD; Teledyne RD Instruments, Inc., Poway, CA, USA) and oxygen optode (Model 3975; Aanderaa Data Instruments AS, Bergen, Norway) were used to describe the physical environment for each image. The location of the ROV above the seabed was determined using an ultra-short baseline acoustic tracking system (TrackPoint-II; ORE Offshore Technologies, Inc.) with a differential GPS (CSI Wireless, Inc. Calgary, AB Canada). All ROV data (e.g., latitude and longitude, physical data) and photographs were synchronously time-stamped and georeferenced. The majority (57.5%) of images were from mesophotic depth zones between 45 and 150 m ( Fig. 2A). The lengths of transects varied depending on the survey type, feature size, and geological characteristics. Survey effort was quantified by summing the lengths of each ROV transect in each depth bin (Fig. 2B). An average of 7,500 images were collected from each of three 50 m interval bins <150 m. An average of 2,500 images were collected from bins between 151 and 350 m. The number of images available per depth class dropped below 1,000 in zones deeper than 351 m.
The size of corals and sponges were recorded as the maximum dimension, height or width, using a laser scale for reference. The corals and sponges were classified into three size categories: 10-20, 21-50, >50 cm. Corals and sponges in each image were classified as "structure-forming" if their height was greater than 10 cm and they exhibited branched morphology; or non-structure-forming (i.e., sea pens, cup corals). The size threshold limited the study to taxa which were large enough to be identified in photos, counted reliably, and potentially subject to impact by bottom fishing gear. Coral and sponge community analysis Richness, evenness, and diversity (Simpson's index, 1/D) were calculated for each site. Raw abundance (counts) was also documented. The number of transects conducted at the 32 sites varied from one to 40, with a mean of 7.72. The Chao 1 estimator was calculated for each site with seven or more dives using rarefaction analysis in Estimate S  (Colwell, 2009). A minimum of 10 dives was required to reach asymptote for most sites. Only dives with >30 unique, on-bottom images deeper than 45 m were used. A total of 247 of the 352 transects met these sample size and depth requirements for the diversity analysis.

Analysis of fishery landings
Landings receipts for commercial fisheries are collected in fishing ports along the coast of California by CDFW. Weight, species, and gear type have been reported and recorded by catch block since 1969 (Fig. S1) and these data have precedent in scientific literature (Miller et al., 2014). Other types of fishing effort data exist from CDFW, such as logbook data, which show start and end points of fishing activity. The data have more precise positioning, but they are confidential. Logbook positions must be averaged over a number of vessels to be shared, compromising the accuracy and precision. Hence, catch blocks for Southern California were used for this analysis, as shown in Fig. S1 (California Department of Fish and Wildlife, 2016). Commercial landings (pounds) from 2007 to 2011 were categorized by species, year, gear type, and catch block (10 min by 10 min catch blocks, approximately 10.5 Â 10.5 miles (17 Â 17 km) in dimension, or approximately 280 km 2 in area) by CDFW's Marine Fisheries Statistical Unit (Perry et al., 2010;Miller et al., 2014). Landings were filtered to include only data from gear types that targeted deep (>50 m) demersal species, and therefore had the potential to interact with coral and sponge communities. These bottom-contact gear types were classified into four major categories (Table 1): trawls, nets (e.g., gillnets), lines (e.g., bottom longlines), and traps (National Marine Fisheries Service, 2011).
A total of 70 species codes of the 356 tracked by CDFW represent deep-water demersal species fished by bottom-contact gear ( Table 2, see Table S1 for scientific names). Landings of the 70 deep-water demersal fisheries included in this analysis were summed over years and joined with catch block polygons using GIS (ArcGIS 10.3; ESRI, Inc., Redlands, CA, USA). Since not all catch blocks were of equal area, landing values were normalized by dividing total landings per block by catch block area (lbs./km 2 ). Landings by catch block and gear type were then visualized using choropleth maps, symbolized by six landings classes defined using Jenks natural breaks (Jenks & Caspall, 1971) for each gear type, for all fixed gear, and for all mobile gear. Commercial landings by catch block were used as an estimator for the distribution and minimum fishing effort throughout the SCB (Miller et al., 2014). Landing data reflect only successful fishing effort, and not true fishing effort.

Spatial overlap between commercial fisheries and deep-water corals and sponges
Spatial overlap between deep-water demersal fisheries and deep-water corals is considered here in three forms: co-occurrence in images of the seafloor; depth range overlap (coincident depth range); and geographic overlap, or coincident latitudinal and longitudinal range. We used ROV images to quantify the number of co-occurrences between fishing gear and coral and sponge fauna. For the purposes of this study, a co-occurrence was defined as the presence of fishing gear debris in the same image as structure-forming corals or sponges. Anthropogenic debris was identified as one of five types: fishing nets, fishing traps, fishing lines, other discarded boating-related gear, or miscellaneous trash. Only the first three debris types were associated with fishing activity. Occurrences were used to examine the depth distributions of predominant corals and sponges in relation to the depth distribution of fishing debris, to examine potential interactions. Since the depths of observations were not normally distributed, a Kruskal-Wallis one-way analysis of variance was used to determine whether the distributions of corals and fishing debris was statistically different. Post hoc, pairwise analysis using Mann-Whitney tests was performed on statistically different depth ranges. All statistical analyses were conducted using R software (R Core Team, 2016).
Visualization of depth distribution was performed using smoothed density curves generated by an algorithm in R software (R Core Team, 2016). The algorithm seeks to estimate an optimal model according to Bayesian information criterion for expectation maximization initialized by hierarchical clustering for parameterized Gaussian mixture models. The approach uses hierarchical clustering to estimate the optimal number of clusters based on the different distributions that make up the overall density structure (Fraley, Raftery & Scrucca, 2012). The estimation assumes that sampling effort is adequate to reflect the true distribution(s). The potential geographic overlap of fisheries and invertebrates was determined by overlaying coordinates of corals, sponges, and fishing debris onto maps of CDFW landings data using GIS. Depth, latitude, and longitude of ROV observations were used to plot the geographic and depth distribution of structure-forming invertebrate taxa and fishing debris. ROV observations were plotted over CDFW fishing blocks in GIS to identify the depths and regions (i.e., catch blocks) where interactions between corals, sponges, and bottom-contact fishing gear are likely to occur.

RAFi index
We used findings from our analyses of coral and sponge communities, fisheries landings, and gear observations to create an index of co-occurrence between deep-water benthic communities and fisheries within the SCB. The index included parameters for richness, abundance, frequency, and fishing intensity. Summary scores for the index, which we called RAFi, were calculated by assigning normalized values (0.00-1.00) for each parameter, and summing the four parameters of richness, abundance, frequency, and fishing intensity equally to yield a maximum possible score (4.00) for each site as follows. The richness "R" parameter used Simpson's reciprocal mean (1/D). The abundance parameter "A" had two types of values: counts of corals and sponges (raw abundance); and frequency of coral and sponges (normalized abundance). In this way, a cumulative value was captured for the total survey extent, as well as a relative value that incorporates differing degrees of search effort. The fishing intensity "Fi" parameter was calculated as the sum of the landings rank (0-5) determined by Jenks natural breaks, plus the normalized frequency of fishing gear observations per ROV image, to yield a maximum score of 1.00.
As an example, consider a study site with the highest diversity of a Simpson's reciprocal mean value of 9.2 (Piggy Bank). Dividing by this maximum value across all sites generates a maximum Richness (R) score of 1.00, and a minimum score of 0.22 (Mission Beach Reef). This process, where score = value Â 1/maximum value, was repeated for the other parameters. Abundance, frequency, and fishing intensity scores had two equally weighted components with maximum subscores of 1.0, where score = [(subscore A + subscore B)/2]. Subscore values for corals and sponges ranged from 0 to 1.0, respectively. These added to a maximum score of 2.0, subsequently divided by 2, to yield a maximum of 1.0. Fishing intensity consisted of a "gear score" and a "landings score." These were also equally-weighted with maximum values of 1.0. The gear score for the study site was based upon the percentage of images that contained fishing gear. The landings score was based on the rank (0-5) of the landings category, divided by 5, to yield values on a scale of 0.0-1.0. Gear scores and landings scores were added together to generate a Fishing intensity (Fi) score ranging from 0.04 (corresponding to a low percentage of images with gear during ROV dives at that site and low reported landings for the block encompassing that site) to 0.97 (corresponding to a high frequency of gear in ROV images at the site and relatively high degree of reported landings). Finally, summary values for the RAFi index were calculated as follows: The final RAFi scores were then ranked to yield the level of priority. A number of iterations took place. Two sets of RAFi tables were calculated, one for all structure-forming taxa, and another for "vulnerable" taxa only, as defined by those taxa in which more than 5% of observations are in the maximum size category, >50 cm. The index was also calculated without fishing intensity values, to determine which sites hosted high diversity and abundance of corals and sponges, regardless of fishing. A map was created once RAFi ranks were determined for sites, and catch blocks were assigned a priority level from high to low (Fig. S1). The levels assigned were: high priority (high landings and many corals or sponges); medium priority (moderate landings and some corals or sponges, or moderate landings with no ROV survey); or low priority (low landings, no evidence of corals or sponges in ROV surveys, or both).

Abundance and diversity of coral and sponge observations
Of the 352 dives analyzed, 331 contained benthic images deeper than 45 m. A total of 26,759 unique photos were analyzed, with 23% containing corals or sponges (6,051). These photos contained 9,610 occurrences (incidences) of corals and/or sponges (Table 3). Scleractinian corals occurred in 1,166 unique images, or 4.4% for a total of 2,060 colonies. Desmophyllum and Coenocyathus were the most commonly observed. The branching scleractinian Lophelia pertusa occurred in 230 images (1%) with a total of 568 colonies counted. The branching hydrocoral Stylaster californicus was observed in 172 images, and 693 colonies were counted. Gorgonian octocorals were the most common corals, they occurred in 2,301 unique images, or 8.6% with a total of 5,136 observed colonies. Sponges were more common than corals. Sponges were observed in 5,736 images (21%), and 12,037 sponges were counted (Table 3).
The coral and sponge assemblages had high richness and evenness (Pielou's J' range of 0.5-1.0). Figure 6 shows the relative proportions of observation for each genus of soft and hard. The number of coral genera and sponge morphotypes found in each study site varied from a minimum of six taxa, in East Catalina Island, to a maximum of 22 taxa, in The Footprint. Since the number of dives in each site varied as well, we used Chao 1 species richness to illustrate diversity at sites where seven or more dives were conducted (Fig. 7). The ROV survey data shows some sites reach 7-10 genera in seven dives, while others rise to 18-20 different genera in seven dives. South San Clemente Island and The Footprint show the greatest rates of accumulation for coral and sponge taxa (>20); 43 Fathom, 9-Mile, and Farnsworth Bank had moderately high richness (15-19); and Potato, Cherry, and Tanner Banks exhibited the lowest number of taxa (10-14).
In general, soft corals comprised the largest size class (>50 cm, Fig. 8A). Gorgonian octocorals Leptogorgia chilensis and Eugorgia rubens, and the black coral Antipathes dendrochristos commonly exceeded 50 cm in size. Acanthogorgia and Paragorgia also exceeded this threshold. All the gorgonian octocorals had a large proportion of colonies in the 21-50 cm size class as well. Among the sponges, the vase-shaped and barrel-shaped morphotypes (e.g., Aphrocallistes and Acanthascus) occasionally grew larger than 50 cm size. The most common morphotypes, foliose and shelf sponges, were generally in the size classes 10-20 and 21-50 cm (Fig. 8B).

Number and types of fishing debris observations
Fishing debris was relatively rare compared to corals and sponges. A total of 914 occurrences of anthropogenic debris (3.4% of photos) were identified, including: 364 images of lines or rope, 292 images of other refuse, 183 pieces of boat gear (all types), 52 images of nets, and 23 images of traps (Table 4). Lines and rope were the most commonly observed anthropogenic debris. "Lines" includes monofilament lines and "rope" includes polypropylene ropes and other types.

Co-occurrences between bottom-contact fishing gear and corals or sponges
A total of 294 co-occurrences of fishing gear and corals or sponges were observed in 914 photos (Table 5). Occurrences with lines/rope were most prevalent (n = 208). Figure 9 shows example images depicting co-occurrences, where fishing gear and benthic fauna were observed. Most co-occurrences involved corals (n = 127), but many 3D sponges (n = 116) also co-occurred with fishing gear. Boat gear were the second most prevalent debris type (n = 54), followed by nets (n = 24) and traps (n = 8). Although sponge observations were more frequent than coral observations, co-occurrences with fishing gear were more common for corals (Table 5). Vertical distribution of corals and sponges Corals and sponges occurred throughout the vertical (depth related) range of 45-475 m depth, but there were significant differences in the depth ranges of individual taxa (Kruskal-Wallis, p < 0.05), as shown in the box plots in Figs. 10 and 11. Corals were stratified into two general groups: a "shallow" (50-100 m) group and a "deep" (100-300 m) group (Fig. 10). Stylaster, Leptogorgia, and Eugorgia were commonly observed in the 50-100 m depth range. Deep gorgonian genera (>100 m) were most frequently represented by the genera Plumarella, Acanthogorgia, Antipathes, and Paragorgia. The "shallow" group also included Adelogorgia phyllosclera, with a depth range of 30-128 m and a median value of 83 m. In pairwise comparisons between shallow taxa, no statistical difference was found between depth ranges of Stylaster, Eugorgia, and Leptogorgia (Kruskal-Wallis, p > 0.05). The depth ranges of Stylaster, Eugorgia, and Leptogorgia were significantly different than those of the "deep" group; consisting of the scleractinian Lophelia pertusa; the black coral Antipathes and the gorgonians Plumarella, Acanthogorgia, and Paragorgia (Kruskal-Wallis, p > 0.05 and Mann-Whitney Z > 1.96). The overall depth ranges for the "deep" group were from 70 to 400 m, with median values from 125 to 275 m and third quartiles reaching to 300 m (Fig. 10). "Bubblegum" octocorals in the genus Paragorgia had the deepest median range and had a statistically different (Mann-Whitney Z > 1.96) depth range than Lophelia and Plumarella, which extend into depths as shallow  as 100 m. Sponges occupied a broad depth range, with a minimum depth of 30 m, a maximum depth of 470 m, and a median value near 150 m (Fig. 11).

Vertical distribution of fishing debris
Debris was identified in still images throughout the depth range of photos, from 27 to 470 m. Median values for traps, nets, and lines/rope were 50-100 m, with third quartiles of traps reaching to 230 m and third quartiles for nets and lines/rope reaching to 150 m (Figs. 10 and 11). Lines and rope had the deepest occurrences among gear types. There was no significant difference in depth range among fishing gear debris types (Kruskal-Wallis, p > 0.05). The highest concentration of debris was generally from Note: Summary of anthropogenic debris types observed as co-occurring with corals and/or sponges. 50 to 250 m depth. Survey effort by the ROV was also highest in this range. Outliers suggest that lines and ropes are likely to occur in deeper waters. Outliers were defined as >1.5 times the interquartile range. These outliers fell between 350 and 500 m depth (Figs. 10 and 11). There was one outlier at 568 m, a single observation of monofilament line.

Depth range overlap between benthic communities and fishing debris
The depth ranges of predominant shallow genera (Leptogorgia, Eugorgia, and Stylaster) were statistically different from the depth ranges of predominant deep genera (Lophelia, Acanthogorgia, Plumarella, and Antipathes) in pairwise Kruskal-Wallis comparisons (p < 0.05). No significant difference was found between depth ranges of fishing gear debris type observations (Kruskal-Wallis, p > 0.05). The depth ranges were statistically similar (p > 0.05) between three predominant coral taxa (Stylaster, Leptogorgia, and Eugorgia) and observations of fishing debris. Corals with deeper ranges did not have statistically equivalent depth ranges with fishing gear debris, although co-occurrences were observed as deep as 500 m. Smoothed density distribution visualizations of predominant taxa and fishing gear are shown on Fig. 12. The histograms for fishing gear show peaks in the same depth range as coral genera Leptogorgia, Stylaster, Eugorgia, and Lophelia.  Table 6). Of the 70 species codes identified as deep-water demersal species, six (sablefish, shortspine thornyhead, ridgeback prawn, hagfish, spot prawn, sea cucumber) accounted for 93% of landings since 2007 (Table 6). Demersal gear accounts for 69-100% of the total reported landings of the top 10 species during this period (Table 6, column 5). Bocaccio, blackgill rockfish, and red rockfish are also taken by gear which typically targets pelagic species. Approximately 9.5 million pounds of seafood were removed from the deep seafloor in the SCB between 2007 and 2011, according to this analysis. Sablefish (Anoplopoma fimbria) and shortspine thornyhead (Sebastolobus alascanus) had the highest landings  Soleidae, but with some Pleuronectidae and Paralichthyidae) were from bottom trawls and account for 30% of total landings. Hagfish (Eptatretus stoutii), spot prawn (Pandalus platyceros), and rockfish (genus Sebastes) were taken by traps and represented about 27% of total landings. Three spatial patterns are evident in plots of demersal fisheries landings from 2007 to 2011. First, landings by bottom trawl fisheries appear to be concentrated in the Santa Barbara Channel region and near the Port of Long Beach, with a few catch blocks reporting landings from San Nicolas, Santa Catalina, and San Clemente Islands (Fig. 13A). Second, fixed gear fisheries (set lines, traps, set nets) appear to have a much broader geographic range in the SCB compared to mobile gear ( Fig. 13B and Figs. S2-S4). Landings for fixed gear fisheries are reported from 147 catch blocks compared to 52 catch blocks for bottom trawl fisheries. Third, the geographic footprint of fixed gear fisheries extends farther offshore than bottom trawl fisheries, especially south and west of the Channel Islands. The mean distance from shore (closest point) for catch blocks with landings larger than 50 lbs./km 2 was 16.4 km (s = 9.2) for trawl landings, while the mean distance for fixed gear landings was 41 km (s = 28.2) from shore.

RAFi indices
The RAFi indices effectively identified sites with high abundance and diversity for corals and sponges, and some evidence of fishing activity. The top 10 ranked sites were robust when fishing scores were excluded, but changed when fishing was included, to up-rank some sites and down-rank others. Indices were examined using the combinations-richness and abundance (without frequency); richness and frequency (without abundance); richness, abundance, and frequency (without fishing components); and the full complement richness, abundance, fishing intensity. The RAFi index scores were based primarily on the numbers of corals and sponges. The total counts are listed by taxon and by bank in supplemental material tables (Tables S2  and S3). Piggy Bank and The Footprint had highest total abundance of corals, and Cherry Bank had high abundance of sponges. Summary scores yielded 32 sites (localities) that were ranked (Tables 7 and 8). Result scores from richness, abundance, and frequency values without fishing components are shown in Table 9.
The "All taxa" method, which included smaller taxa, ranked Piggy Bank, 9-Mile Bank, and South San Clemente Island as highest for species diversity. Piggy Bank, The Footprint, Cherry Bank, and 43-Fathom Bank scored highest for abundance of corals and sponges. Piggy Bank, The Footprint, and Del Mar Steeples scored highest for frequency of corals and sponges. Farnsworth Bank, West Santa Catalina Island, and Del Mar Steeples scored highest for fishing intensity. When the ranking was repeated using only vulnerable taxa, which excluded some of the smaller taxa, the top scores for richness (in order) were Piggy Bank, South San Clemente Island, and 9-Mile Bank; the top scores for abundance were Piggy Bank, The Footprint, and 43-Fathom Bank; the top scores for frequency were Piggy Bank, The Footprint, and Del Mar Steeples; and the top scores for fishing intensity were West Santa Catalina Island, Farnsworth Bank, and Del Mar Steeples.
When summed RAFi scores were tallied for all the larger structure-forming taxa, the top six (upper quartile) ranked were Piggy Bank, The Footprint, West Santa Catalina Island, 9-Mile Bank, 109-Seamount, and South San Clemente Island. Upper quartile ranking for vulnerable taxa were Piggy Bank, West Santa Catalina Island, The Footprint, Farnsworth Bank, South San Clemente Island, and 9-Mile Bank. The ranking results of both exercises were highly correlated (R 2 -0.988) and not statistically different (p = 0.03; p < 0.05). Six sites consistently ranked the highest even when fishing intensity values were removed from the total RAFi score.

Geographic overlap between deep benthic communities and demersal fishing
Corals and sponges were observed in many of the blocks from which demersal fisheries landings were reported, particularly near San Diego and several offshore islands (Figs. 13A and 13B and Figs. S2-S4). The specific fisheries operating in overlapping catch blocks and the types of structure-forming invertebrates occurring in those catch blocks are     summarized in Table 10. The highest landings were reported from trap and line fisheries.
Trap fisheries took prawns, sablefish, and hagfish, with highest landings at Port Hueneme and San Clemente, the latter of which had many documented species of corals (Osada & Cailliet, 1975). The line fisheries took sablefish, thornyheads, and rockfish, with highest landings at Santa Catalina, Piggy Bank, and San Clemente all with records of corals. Trawl landings were highest at the Port of Long Beach and Port Hueneme, but also reported at San Clemente and throughout the Bight.

California demersal fisheries and coral observations in recent reports
This study took a similar approach to the estimation of fishing effort off California as Miller et al. (2014) did in the broad assessment of fisheries distribution along the West Coast, but differs in that the work here focused on Southern California's deep-water landings, and included benthic invertebrates (sea cucumbers, rock prawns) and non-groundfish (hagfish, soupfin shark). This is an important difference because invertebrates represent 31% of recent landings since 2007. Miller et al. (2014) established a good precedent for scientific application of CDFW data, showing that historical landings and catch per area were relatively low in Southern California groundfish fisheries compared to Northern California. This suggests that the Southern Californian seafloor may be generally less impacted and more pristine than seafloor habitats in other regions. The reduced impact would help to maintain diverse and abundant benthic epifauna, such as the deep-sea corals and sponges observed in our study. All the coral genera reported here were previously known to occur in the SCB, but this report adds new information on their geographic and vertical distribution and helps to prioritize particular localities for management. Two different assemblages of azooxanthellate corals were identified: a "shallow" (50-100 m) and a "deep" (100-400 m) assemblage. The reef-forming hydrocoral Stylaster californicus was found to be the predominant hard coral in shallower depths. Stylaster appeared to occupy the same   (1) high priority (high landings and many corals); (2) medium priority (moderate landings and some corals); (3) medium priority (moderate landings with no ROV images); or (4)  vertical niche as four gorgonians, Leptogorgia chilensis, Eugorgia rubens, Adelogorgia phyllosclera, and Muricea spp. Slightly deeper waters (>100 m) hosted a different assemblage consisting of Antipathes dendrochristos, Paragorgia sp., and Lophelia pertusa (now Desmophyllum pertusum), as well as Plumarella sp., and Acanthogorgia sp.
Overall, the composition of the SCB coral assemblage is diverse, with at least twelve structure-forming deep-sea coral genera. Several observations of gorgonian corals could not yet be identified by photos alone, or may represent undescribed taxa. An important caveat to consider is that coral genus and sponge types were identified based on the limits of visual (photographic) observations and taxa known to exist in the region based on previous surveys where samples were collected. The ROV surveys employed for this study were originally visual surveys for white abalone and groundfish stock assessments, where no biological samples of corals or sponges were collected to verify identifications. However, data derived from these surveys can be used to pinpoint large aggregations of deep-sea corals at the genus level because the genera have distinct coloration and branching patterns. The information can be used in future studies to guide collections of voucher specimens for morphology, genetics, population connectivity, age, and growth studies. More fieldwork is also required to target areas where corals have not been documented but may be expected to occur.
Further analysis of the image data remains to be done. Sponges were enumerated, but only identified by morphological type. Fishes and other benthic megafauna remain to be enumerated. Coral health and condition, size-class structure, and other aspects may also be ascertained from the substantial collection of images amassed by NOAA's Southwest Fisheries Science Center. The images are now publicly available through NOAA's National Database of Deep-Sea Corals and Sponges and may be employed for further analyses (NOAA, 2017).

Overlap of fisheries and deep-sea corals
Three types of spatial overlap were proposed as a framework for understanding co-occurrences of commercial fisheries with corals and sponges in the SCB: co-occurrences (in the same frame) from images of the seafloor, depth range overlap, and geographic overlap. The co-occurrence of corals, sponges, and fishing debris in ROV photos was observed directly and quantified by study site. Depth range overlap was illustrated through an analysis of depth distribution of corals, sponges, and fishing debris observed in benthic ROV survey data. Broad geographic overlap was also illustrated empirically by overlaying coral and sponge occurrences with fisheries landings effort. Depth and geographic area overlaps have a coarse spatial scale. Many seabed types can occur in a single catch block. Different assemblages can be expected to occur in each seabed (or community) type. The highest likelihood of interactions would be expected in rocky habitats, when the preferred habitat of commercially fished species (e.g., rockfish, spot prawn) overlaps with the preferred habitat of corals and sponges.
Future studies should seek to access or generate fine-scale (1-5 m resolution) benthic maps that delineate soft-bottom from hard-bottom in order to avoid accidental incursions into hard-bottom habitats by fisheries targeting soft-bottom areas. It would also be helpful to survey fishermen to ask about the habitat preferences of their target species, and whether fishing efforts targeting certain seabed types are indeed restricted to those types.
From this analysis, it appears that depth range overlap does exist between bottom-contact fisheries and corals and sponges throughout the SCB. The majority of fishing debris in this study was observed in the 50-250 m depth range. This is consistent with the depth range of the largest demersal fisheries in the SCB in terms of total landings, including: set line fisheries for sablefish and shortspine thornyhead, bottom trawls for ridgeback prawn, and traps for hagfish and spot prawn. The 50-250 m depth range is also consistent with the depth range of several species of corals, including Stylaster californicus, Lophelia pertusa, Antipathes dendrochristos, Leptogorgia chilensis, Eugorgia rubens, Adelogorgia sp., Plumarella sp., and Acanthogorgia sp. The bubblegum coral Paragorgia is slightly deeper than this range, but there are reports that commercial and recreational rockfish fisheries have been moving into deeper waters (Leet, 2001;Miller et al., 2014) where Paragorgia and other fragile sea fans are expected to occur.
In terms of geographic overlap, these results show that mobile gear has a relatively small footprint in the SCB compared to fixed gear, and there is considerable overlap between fisheries landings and the occurrence of corals and sponges for both mobile (trawl) and fixed gear. Fixed gear is more often used in complex, rocky areas whereas trawl gear is more often used in broad flat areas. Complex rocky areas are a hazard for trawl gear. It is evident from this study that trawling has occurred since 2007 in some of the same catch blocks as corals and sponges; however, in situ photo observations employed for this study were focused primarily on offshore habitats. So, the true extent of the overlap in the Santa Barbara Channel is not fully realized. An aggregated dataset combining multiple resources (e.g., observations from towed cameras and submersibles) is necessary to deduce the full extent of geographic overlap in nearshore habitats in the northern SCB where most trawl landings originate. NOAA's Deep-Sea Coral Research Technology Program currently assembles this type of public resource (NOAA, 2017).
A large portion of fixed gear landings occur offshore compared to mobile gear, and a large number of in situ observations of corals and sponges were also offshore. Our analysis of co-occurrence from ROV photos showed that lines and rope were the most commonly observed fishing debris based on the frequency of occurrence and the frequency of interactions with corals and sponges. Bottom longline fisheries are an emerging concern for deep-sea coral management because of coral bycatch in these fisheries (Sampaio et al., 2012). Line fisheries have traditionally not been a concern because "area swept" is small compared to mobile gear. However, entanglements do occur, and the total landings by those fisheries are high, so cumulative impacts may bear investigation (Rooper et al., 2017). Traps also have the potential to damage corals and sponges on deployment and recovery, especially when strung together.

Priority sites for conservation-and management-based research and monitoring
A qualitative analysis was used to prioritize catch blocks in terms of potential spatial overlap with observations of deep, structure-forming corals and sponges based upon (1) the abundance and richness of corals and sponges, (2) the relative magnitude of landings within each gear type, and (3) the number of different gear types used in each block. Three priority types emerged from this analysis: high priority (high landings and many corals); medium priority (moderate landings and some corals or moderate landings with no ROV images); and low priority (low landings, no evidence of corals or sponges in ROV surveys, or both) (Fig. S1).
Highest priority recommendations for conservation-focused research of deep-water corals include sites off San Clemente Island and Santa Catalina Island, particularly in relation to San Clemente Island's fishery for red sea cucumber in block 850; trap fishery for spot prawn in blocks 867 and 829; and set line fisheries for sablefish, rockfishes, and thornyheads in blocks 829 and 830. Block 830 has some fishing but no survey data. Waters within fishable depths around Santa Catalina are closed to bottom trawling. Santa Catalina Island's set line and trap fisheries in block 762 (high landings, many corals) and blocks 761 and 807 (no survey data) should also be a focus for future research. In addition, block 683 near Port Hueneme has the highest reported landings of demersal species, but no ROV survey data.
Medium priority recommendations for conservation-focused research of deep corals include catch blocks 813 and 814 near San Nicolas; catch blocks 707, 708, and 710 in Channel Islands National Marine Sanctuary (CINMS); and the adjacent catch block 730. San Nicolas reports moderate to low landings in trap fishery for spot prawn, trawl fishery for red sea cucumber, and set net fishery for soupfin sharks. CINMS catch blocks 707 and 708 have moderate landings in the set line fisheries for sablefish, rockfish, and thornyhead. Piggy Bank and The Footprint are mound and ridge features encompassed by these catch blocks. The features have high abundance of corals and sponges. Piggy Bank was the subject of recent research surveys sponsored by NOAA's Deep-Sea Coral Research and Technology Program (Yoklavich et al., 2011). The Footprint was the subject of benthic surveys by Delta submersible (Bright, 2007).
Further west in CINMS, near Santa Rosa, catch blocks 710 and 730 have low landings, but some occurrences of sponges and gorgonians that indicate suitable habitat. Other regions where fishing is occurring but no survey data is available include block 740 off Long Beach, block 711 off Santa Rosa, and block 807 off Santa Catalina (see Table 10, Rank 3).

Observations of debris in other areas
Unfortunately, anthropogenic marine debris is relatively common along the California coast (Watters et al., 2010) where deep-water corals and sponges occur. In that study, the relative abundance of debris was much higher in central California compared to the SCB. In central California, most observations of debris (over 90%) were of recreational fishing gear (e.g., monofilament fishing line) in rocky habitats compared to the SCB where commercial fishing gear (e.g., longlines, nets, traps, and empty bait cans) and maritime debris (e.g., beverage containers and construction materials) comprised a large portion of observations. There was little indication in Watters et al. (2010) that the observed debris had caused disturbance to the seabed habitats and/or benthic megafauna present. However, in this study there were 294 examples of co-occurrence with corals and sponges. Co-occurrence was defined as presence of fishing gear and corals or sponges in the same photo. Co-occurrence does not necessarily mean disturbance, but shows derelict gear in close proximity, or in physical contact with benthic fauna observed. Lines and rope were the most common type of debris, constituting nearly 71% of gear co-occurring with structure-forming corals and sponges in this study.

Site-based prioritization using RAFi index
California Department of Fish and Wildlife landings are reported by catch block, not geographic location, so although fishing may clearly be taking place, fishing may not be taking place in the same habitat type as corals and sponges. For example, rocky substrates favored by spot prawns and certain rockfish are similar to coral and sponge habitats observed. Other target fisheries like sea cucumber and ridgeback prawn favor soft sediments where corals and sponges are less common. Overlap of commercial landing records by catch block with coral and sponge observations can provide some means for the development of effective, priority-based management efforts. However, when determining very refined boundaries for potential HAPCs, catch block-based managements will not distinguish seafloor features or substrate variation within a grid cell. Any evidence from direct observations of fishing gear and coral interactions are also not taken into account using a catch-block approach.
In addition to spatial overlap, the RAFi index incorporated data from frequency of fishing debris observations, as well as diversity of sessile epifauna, to achieve an array of quantitative criteria for conservation and management-based research and monitoring prioritization. Our top results from the RAFi index score (Tables 7 and 8) coincide with priority blocks for Piggy Bank, The Footprint, Western Catalina Island, and Southern San Clemente Island (Table 10 and Fig. S1). When fishing is excluded, the study sites at South San Clemente, Santa Rosa Flats, 9-Mile Bank, Tanner Banks, Cortes Bank, and West Catalina are all up-ranked priorities in the top 10, while and Del Mar Steeples and Farnsworth Bank drop in rank (Table 9). The RAFi index is therefore flexible and useful for a variety of prioritization approaches.

CONCLUSIONS
In conclusion, this study shows that deep-water corals and sponges are broadly distributed offshore and throughout the SCB, from Point Conception in the north to San Diego in the south. The general depth ranges of corals observed in this study (45-500 m) are consistent with the depth ranges of commercial fishing debris and the depth ranges of bottom-contact fisheries as reported by the state fisheries agency.
As reported in Rooper et al. (2017), the spatial extent of fixed gear fisheries in Southern California is found to be larger than the extent of trawl fisheries, which is perhaps of some concern for corals and sponges because most co-occurrences were from lines. More information is needed on the distribution of corals and sponges precisely where fixed gear and trawl fisheries operate in the SCB. Any rocky outcrop offers potential habitat to deep-sea corals and sponges (Hardin et al., 1994). There is also a need for more information on the absence of corals and sponges where bottom-contact fisheries occur.
This study assigned priority ranks based on a conservation priority index called "RAFi" scores. An index such as RAFi can be a useful decision support tool for marine managers to help focus habitat conservation efforts. In this case, the ranking exercise indicated that an existing network of State marine reserves, National marine sanctuaries, EFH, and Rockfish conservation areas (RCAs) (Pacific Fishery Management Council, 2006) already encompasses most of the highly-ranked localities, and this limits impacts from bottom-trawling. Based upon data examined, the Channel Islands region, San Clemente Island, Santa Catalina Island, and sites offshore like Seamount 109 should be a high priority for research and management. In these places, diversity and abundance of deep-sea corals and sponges is high, and the bottom fishing restrictions in place in these areas are impermanent, and routinely under review by the regional Fisheries Management Councils. The benthic communities at Cortes Bank, Tanner Bank, and Cherry Bank had moderate diversity, high abundance and low fishing pressure, so these ranked as intermediate priorities, but they gain importance if fishing intensity is not included. The Cowcod Conservation Area encompasses these outer banks.
The largest coastal protected area in Southern California at the time of this writing is the RCA, which encompasses many highly-ranked localities. The extent of the RCA fluctuates seasonally, and the regulation is impermanent. Wherever RCA is in place, de facto protections from bottom trawling may be expected to provide benefits to deep-sea corals and sponges, as well as rockfish. A new area called the SCB EFH conservation area is now recommended by the Pacific Fishery Management Council (2018) to permanently restrict trawl gear where most deep-water coral and sponges occur in the SCB. The proposal will not restrict bottom-contact gear such as longlines, pots, and traps, which are most likely to be found in these hard-bottom ecosystems. Ongoing dialogue with fishermen, improved habitat maps, and continued benthic surveys are recommended to determine where fisheries interactions occur and whether deep-sea corals and sponges are adversely affected.