Distributed under Creative Commons Cc-by 4.0 the Physiological Consequences of Varied Heat Exposure Events in Adult Myzus Persicae: a Single Prolonged Exposure Compared to Repeated Shorter Exposures

The study of environmental stress tolerance in aphids has primarily been at low temperatures. In these cases, and in the rare cases of high temperature tolerance assessments, all exposures had been during a single stress event. In the present study, we examined the physiological consequences of repeated high temperature exposure with recovery periods between these stress events in Myzus persicae. We subjected individuals to either a single prolonged three hour heating event, or three one hour heating events with a recovery time of 24 h between bouts. Aphids exposed to repeated bouts of high temperatures had more glucose and higher expression of proteins and osmolyte compounds, such as glycerol, compared to the prolonged exposure group. However, aphids exposed to the repeated high temperature treatment had reduced sources of energy such as trehalose and triglyceride compounds than the prolonged exposure group. Recovery time had more physiological costs (based on production of more protein and consumption of more trehalose and triglyceride) and benefits (based on production of more osmolytes) in repeated high temperature treatments. As aphids are known to respond differently to constant versus 'natural' fluctuating temperature regimes, conclusions drawn from constant temperature data sets may be problematic. We suggest future experiments assessing insect responses to thermal stress incorporate a repeated stress and recovery pattern into their methodologies.


INTRODUCTION
Climate change is one of the most critical threats to biodiversity (Dawson et al., 2011;IPCC, 2014). Human-induced climate change is predicted to increase the frequency of climatic extremes (e.g., heat waves and severe droughts or floods), climatic variability (Lean & Rind, 2008;Rahmstorf & Coumou, 2011) and mean of the thermal environment in regions around the world (Niehaus et al., 2012). Global mean temperature has risen by 0.85 • C from 1880 to 2012. All of the warmest 20 years on record have occurred since 1990 (CSIRO-ABM, 2014).
Exposure to different thermal means and variability can have a substantive influence on animal physiological performance (Huey et al., 2012). This may include a modification enzymes); changes in membrane structure; and osmolyte compounds; to survive and recover from unfavourable conditions (Back, Oakenfull & Smith, 1979;Henle, Peck & Higashikubo, 1983;Lin, Hefter & Ho, 1984;Kim & Lee, 1993;Meng et al., 2004;Wang et al., 2006;Clark & Worland, 2008;Tollarová-Borovanská, Lalouette & Koštál, 2009). Aphids and whiteflies were one of the first insect taxa reported to accumulate polyols in response to high temperatures and also reveal that these compounds could stabilize proteins structure against thermal denaturation (Back, Oakenfull & Smith, 1979). Whole-animal respiration will change when exposed to repeated stress (Lalouette et al., 2011;Yocum et al., 2011) and understanding the implications of this is critical as this will impact on animal survival and body maintenance as changes occur in energy and water usage (Schimpf et al., 2009;Chown, Sorensen & Terblanche, 2011). In addition, the upper critical temperature threshold at which an animal loses muscular control (CT max ) is a metric of interest as it is useful in predicting phenotypic effects of warming (Huey et al., 2012). Using laboratory estimates of thermal tolerance enables the seasonal abundance and geographic distribution of organisms to be determined (Ju et al., 2013).
It has become clear that constant temperatures are not useful for study performance thermal response of organisms as they do not mimic the fluctuating temperature conditions which occur within the natural environment (Lamb, 1961;Davis, Radcliffe & Ragsdale, 2006). Constant temperature conditions also underestimate thermal thresholds of individuals and become less accurate compared to fluctuating temperature regimes (Davis, Radcliffe & Ragsdale, 2006;Niehaus et al., 2012). This implies that fluctuating temperature experiments ought to be ''normal'' while constant temperature insect development studies were ''abnormal'' experimental conditions (Cloudsley-Thompson, 1953). Fluctuating temperatures enhanced resistance of leaf beetles and fruit-flies to low temperature (Casagrande & Haynes, 1976;Meats, 1976) and that fitness could be more significant in repeated temperatures (Beardmore & Levine, 1963).
In previous studies much attention has focused on the cold tolerance of Myzus persicae (O'doherty & Bale, 1985;Bale, Harrington & Clough, 1988;Clough, Bale & Harrington, 1990;Howling, Bale & Harrington, 1994;Bezemer, Jones & Knight, 1998;Vorburger, 2004) as well as assessments of the effects of temperature on physiological parameters including their thermal tolerance at different latitudes and altitudes (Bezemer, Jones & Knight, 1998;Alford, Blackburn & Bale, 2012a). There is a paucity of knowledge regarding how aphid physiology is affected by repeated exposure to high temperatures. Here, we assessed aphid metabolic rates after different heating regimes (a single prolonged exposure and repeated short-term exposure with recovery time, and no heat exposure) using flow-through respirometry; we also assessed the upper critical temperature threshold, energy reserves and their osmolyte compound profile, as indicators of aphid stress response after exposure to the different heating regimes.
We predict that repeated short-term exposure to high temperatures will increase adult aphid thermal tolerance, as recovery time between heat stress bouts will enable metabolic and cellular repairs to occur (Storey & Storey, 2004). We also predict that aphids will accumulate polyols and sugars in response to high temperatures (Hendrix & Salvucci, 1998) which will result in an increased CT max . Figure 1 Diet cages. These were constructed from rigid clear PVC plastic tubes, 12 cm in diameter (aphid feeding) and covered with a 7 × 7 cm sheet of Parafilm laboratory film.

Aphids and diet chamber construction
Stock colonies of Myzus persicae (green peach aphid), were collected from canola plants at the Laureldale Farm property (University of New England, Armidale, NSW Australia) in 2013.
The lab colony were established from an isofemale line (BG_13-001) and maintained on canola variety 'Thunder Bay' plants in a glasshouse with 25 ± 0.5 • C temperatures, 65% relative humidity, under a 16:8 h (L:D) photoperiod provided by fluorescent lamp for two years to ensured continuous apomictic parthenogenesis.
Since aphids have parthenogenetic embryogenesis (Dixon, 2005) we bred three generations of aphids in the lab to reduce maternal and grand maternal affects: we removed the wingless female adult aphids after 3 days, then the resulting nymphs were left for seven days and we continued this process for three generations. The third generation resulting nymphs were housed at 25 • C, 65% relative humidity, under a 16:8 h (L:D) in a Thermoline incubator (TRH-300) prior to experimental manipulation.

Diet cage preparation
To rear our aphids on artificial diets we used diet cages. Diet cages were constructed from rigid clear PVC plastic tubing with a 12 cm diameter (Fig. 1). A 7 cm × 7 cm square of parafilm was extended by force across the top of a cage, and 2 ml of artificial diet was pipetted on top of the parafilm layer. A second 7 cm × 7 cm sheet of parafilm was then extended over the first sheet, forcing diet across the top surface of the cage, but avoiding leakage over the edge. Then by using a paintbrush, aphids were placed on the underside of the parafilm. The diet cages where then put into a closed transparent box (30 × 30 cm) and put into the incubator prior to the application of treatments.

Artificial diet
The artificial diet used to rear the aphids was based on work by Douglas (1988) and consisted of specific concentrations of phosphate, vitamins, and minerals (Table 1). In the experiments, four replicate groups of 200 larval aphids were maintained on the test diet for 5 days (i.e., from one to 6 days of age). The specified amounts of each component (see Table 1) were prepared with distilled deionized water to the total volume of 10 ml in a glass container. The pH of the solution was 7.0-7.5. The diet solution was divided into 2 ml aliquots, and stored at 4 • C s for less than 1 week or at −20 • C for less than three months. In all experiments, distilled deionized water was used in all of the solutions and the diets of the aphids were changed twice a week for the duration of our study.

Experimental conditions
To investigate the effects of fluctuating thermal regimes (FTR), the experimental design was a simplified version of the experimental protocol of Marshall & Sinclair (2011) and Zhang et al. (2011). For the repeated heat stress exposure treatment, adult aphids were exposed to one to three diurnal cycles (We left them in the food diet and put them into the incubator) of 1h at 38 • C (we didn't control RH) followed by 24 h at 25 • C, 65% relative humidity (RH). Adult aphids (seven day old individuals) from the third generation reared on the artificial diet were used. For the single prolonged heat exposure treatment, separate groups of adults were exposed to 38 • C for 3 h, to make total heat exposure time equivalent among treatments. Post treatment, this group were also given a 24 h-period of recovery at 25 • C, 65% RH. Meanwhile, control animals were held at 25 • C, 65% RH for the duration of the study. Estimates for the upper critical temperature threshold limits for M. persicae have previously found to range from 38.5 • C (Broadbent & Hollings, 1951) to 42 • C (Hazell et al., 2010). Myzus persicae are known to survive 1h per day above their CT max of 38.5 • C (Davis, Radcliffe & Ragsdale, 2006) and so the FTR was chosen to fluctuate around a value close to their CT max . In general, upper temperatures are difficult to experiment with compared to lower temperatures because their performance curve optima is very close to CT max . A test temperature of 38 • C was chosen as there was 60-70% survival following the prolonged thermal exposure for 3 h.

Quantification of metabolic reserves
Twenty-four hours after a heat exposure (Fig. 2), four replicate groups of adult aphids were weighed to 0.001 mg on a Mettler Toledo XP2U (Switzerland) electronic balance, then homogenized in 80 µl extraction buffer (35 mM Tris, 25 mM KCl, 10 mM MgCl2, pH 7.5) with 0.1% (v/v) Triton-X-100 after centrifuging for 1 min at 13,000 rpm at 4 • C. Supernatant was then removed and placed in a new tube which was then stored at −20 • C until all assays were conducted.
For these assays, we measured Glucose, Triglyceride, Glycerol, Trehalose and total protein content in triplicate and we used a visible wavelength spectrophotometer (Epoch Microplate Spectrophotometer; BioTek, Winooski, VT, USA) with absorbance at 544, 540 and 750 nm and 96 well plates, with volumes scaled down from the manufacturer protocols (Ridley et al., 2012).

Active metabolic rate measurement (AMR)
Flow-through CO 2 -based respirometry was used to record V CO 2 , with a similar experimental setup as described by Terblanche & Chown (2007). A HiBlow HP40 air pump All experiments were performed on adult aphids, that were 6 days old on the first experimental day. Each black rectangle represents a 1 h exposure to 38 • C. The blue rectangle represents a 3 h exposure to 38 • C. Adults were kept at 25 • C as the control group (yellow line). Red dots indicate sampling points. All samples were collected 24 h after final treatments. was used to feed atmospheric air into sodalime (VWR with indicator AnalaR NORMAPUR analytical reagent) and Drierite (WA Hammond Drierite Company) scrubber columns, to remove CO 2 and water vapour from the air stream, respectively. The flow rate of the airstream was regulated at 80 ml min −1 by a flow control valve (Model 840, Sierra Side-Trak; Sierra Instruments Inc., Monterey, CA, USA), connected to a mass flow controller (Sable MFC-2). Thereafter, air flowed through the zero channel (cell A) of a calibrated (to 6 ppm CO 2 in air) infrared CO 2 -H 2 O analyzer (Li-7000; Li-Cor, Lincoln, NE, USA). The airstream then flowed over the test animal in the 5ml glass cuvette, which was placed in a programmable water bath (Grant, GP200-R4), programmed using LABWISE software with increasing temperature of 0.25 • C min −1 (see Basson & Terblanche, 2010). The air leaving the cuvette then entered the analyser through another channel which recorded the difference in CO 2 concentration of the air before and after it flowed through the cuvette, at 1 s intervals. Changes in animal position (activity) were recorded using an infrared activity detector (AD-1; Sable Systems, Las Vegas, NV, USA). Aluminium foil was placed around this cuvette to restrict light exposure and to ensure high quality activity recordings (MacMillan et al., 2012).
Four aphids per replicate were weighed to 0.001 mg on an electronic microbalance before and after the experiment and mean mass used as a covariate in statistical analyses. The output from the CO 2 analyzer and activity data were recorded with the LiCor 7000 software and analysed using Expedata V1.25 software (Sable Systems International, Las Vegas, NV, USA). Volumes of CO 2 in ppm were corrected for baseline drift and then converted to µl CO 2 h −1 using Expedata software. Rates of CO 2 production (in µl CO 2 h −1 ) were calculated from the whole record by transforming ppm concentration of CO 2 to CO 2 fraction and then multiplying by the flow rate (80 ml min −1 ). The area under the curve (integral of ml CO 2 min −1 vs min) was calculated. This area was equal to the volume of CO 2 produced by each replicate in the cuvette, and this volume was divided by the total period of measurement (2.30 h), multiplied by 1,000 to give µl CO 2 h −1 to give the metabolic rate per aphid per hour (Castañeda et al., 2009). Metabolic rate was measured for each of the four times two replicate aphids after the final exposure in all experimental groups.

Measuring upper critical thermal limits (CT max )
Critical thermal limits were determined by subjecting aphids to a regime of increasing temperatures and monitoring their ability to control their movement: loss of muscular controls to determine their CT max threshold. Forty adult aphids were each placed within an individual 5 ml plastic tube at a pre-set temperature (25 • C). A programmable water bath was set to increase the temperature from 25 • C to 35 • C with a rate of 0.5 • C min −1 then the temperature was increased to 45 • C with a rate of 0.1 • C min −1 to minimize the hardening response across a broad range of upper critical temperatures and to determine the temperature at which walking ceased and aphids succumbed (Hazell et al., 2008;Alford, Blackburn & Bale, 2012b). Upper critical temperature was measured for 40 adult aphids for each of the three experimental treatments after final heat exposure in all experiments group.

Statistical analysis
All data were analysed for normality and tested for homogeneity of variances for treatment means using the Levene's Test, in the HOVTEST option of GLM procedure within SAS software (2008). A completely randomized design was employed. One-way analysis of variance was performed using the GLM procedure. Tukey post hoc tests were used to compare means (P < 0.05).

Glucose content
The glucose content of the control group exhibited no significant differences over the course of the experiment (Day 1: 1.61 ± 0.02 to Day 3: 1.96 ± 0.06 µg glucose mg −1 (Fig. 3A)). However, adult aphids in both the prolonged and repeated exposure groups experienced a significant increase in glucose content. After a single cycle of 38 • C for 1h and 25 • C for 24 h, glucose content increased significantly, nearly one and half fold for aphids in the repeated exposure group (P < 0.0001; Table 2). For the duration of the experiment, the glucose content of aphids in the repeated exposure group peaked at 3.26 ± 0.02 µg glucose mg −1 (Fig. 3A). For adult aphids continuously exposed to 38 • C for 3 h (prolonged exposure treatment), glucose content significantly increased compared to the control group (3.01 ± 0.02 µg glucose mg −1 ).

Treatments
Treatments Contrasts

Trehalose content
The trehalose content of aphids exposed to repeated heating events significantly decreased from 0.44 ± 0.01 to 0.27 ± 0.01 µg trehalose mg −1 after the second heating exposure (Fig. 3B). After three cycles of heating, trehalose content of aphids was significantly lower than the control group on the third day of the experiment (P < 0.001; Table 2). For aphids exposed to 38 • C for 3 h, the trehalose content did not significantly differ (P = 0.329; Table 2) from that of the control group.

Protein content
Similar to glucose and glycerol content, protein content of aphids that were heated during repeated exposures was significantly higher than in the control group and their prolonged exposure counterparts (P < 0.001; Fig. 3C). After one cycle of 1 h at 38 • C and 24 h at 25 • C, the protein content of aphids was 17.16 ± 0.06 µg protein mg −1 , but this value steadily increased every cycle, and by the end of third cycle protein content was 19.79 ± 0.1 µg protein mg −1 (Fig. 3C). Protein content in aphids heated at 38 • C for 3 h did not significantly differ from the control (Fig. 3C).

Glycerol and triglyceride content
Triglyceride content in aphids did not significantly differ between control and the prolonged exposure groups. However there was a significant drop of triglyceride content in aphids after three cycles of repeated heating in the repeated exposure group: triglyceride content in the repeated exposure group was less than half that of control (0.659 ± 0.01) and prolonged (0.597 ± 0.01) aphids and reached 0.324 ± 0.02 µg triglyceride mg −1 (P < 0.002; Fig. 3E). Glycerol content was significantly higher (P < 0.0001; Table 2) in aphids of the repeated exposure group increasing from 1.53 ± 0.01 to 1.96 ± 0.02 µg triglyceride mg −1 after three cycles of heating. There was also significantly higher glycerol content in aphids of the prolonged exposure group compared to the control group (P = 0.0001; Fig. 3D).

Thermal tolerance
There was no significant difference in aphid CT max between the thermal treatments (Fig. 4).

Active metabolic rate measurements
There was no significant difference in metabolic rate 24 h after heating among treatments (P = 0.6; Fig. 5A). Aphids from the repeated heating and prolonged heating treatments exhibited significant water loss compared to the control group (P = 0.04; Fig. 5B).

DISCUSSION
In the present study, we examined the physiological consequences of either being exposed to repeat short-period high temperatures bouts with recovery time periods between them, or a single prolonged temperature treatment, in the aphid Myzus persicae (Fig. 2). We observed increased physiological costs and benefits during repeated heating exposure: this group of aphids had more glucose and higher expression of proteins and osmolyte compounds such as glycerol compared to the prolonged exposure group. However, the repeated high temperature exposure group also had fewer sources of energy such as trehalose

Figure 5 Mean (±s.e) (A) CO 2 production (µl CO 2 h −1 ) and (B) H 2 O output rates (µg H 2 O h −1 ) of aphids among control (C), prolonged (P) and repeated (R) exposure treatments.
Metabolic rate based on four times two replicate. Post-hoc pairwise differences among treatments indicated by stars. and triglyceride compounds compared to the prolonged exposure group. We found that recovery time had more physiological costs (based on production of more protein and consumption of more trehalose and triglyceride) and benefits (based on production of more osmolytes) for repeated high temperature exposure group, but interestingly we saw no changes in thermal tolerance and metabolic rate across treatments.

Impacts of repeated high temperature on thermal tolerance of aphids
Fluctuating temperature studies are better at predicting the thermal tolerance of aphids than constant temperature studies, even when the mean temperatures are the same between constant and fluctuating regimes (Lamb, 1961); constant temperature studies also underestimate critical temperature thresholds as it is known that fluctuating temperatures develop threshold limits (Casagrande & Haynes, 1976;Meats, 1976). The upper critical temperatures in M. persicae start from 38.5 • C (Broadbent & Hollings, 1951) to 42 • C (Hazell et al., 2010). To our knowledge just two studies (Davis, Radcliffe & Ragsdale, 2006;Gillespie et al., 2012) have assessed fluctuating high temperatures in M. persicae: but our study is the first to assess physiology of aphids at high temperatures. Davis, Radcliffe & Ragsdale (2006) examined the effect of high and fluctuating temperatures on the development of M. persicae. They demonstrated that under fluctuating temperatures, M. persicae had greater fertility and faster development and had the capacity to survive 1 h every day above the CT max of 38.5 • C (Davis, Radcliffe & Ragsdale, 2006). These results are in contrast to Gillespie et al. (2012) who found that under heat wave conditions, the growing population in aphids was lower when exposed to heat waves than weather with periodic hot days. Therefore, differences in their results may be due to other factors such as changes in mean temperatures.
Extreme temperature exposure in short bursts often increase survival compared to prolonged exposure. At optimum conditions, injury repair can occur by restoring ion homeostasis (Kostal et al., 2007) and replenishing energy levels. In Drosophila melanogaster, Krebs & Loeschcke (1994) found similar results for effects of heating in adult female flies by exposing them to 36 • C 1-3 times with 48 h rest between heating exposure. Their results showed that exposure to bouts of stress increased survival temperature to 39 • C. Furthermore, recovery time at optimum temperatures may have permitted aphids to recover from the negative impacts of high temperatures (Hazell et al., 2010). In our study, it appears that recovery times between high temperatures can repair injuries, as aphids exhibited higher levels protein and osmolyte in the repeated exposure treatment compared to the prolonged exposure treatment group: but there was no significant difference in their CT max between treatments. One possible explanation is that the proteins and osmolytes were upregulated during the recovery time, but upregulation was not great enough to change their thermal threshold between treatments (Tammariello, Rinehart & Denlinger, 1999). One further reason for conservatism of physiological resistance to heat is due to upper thermal tolerance being largely uncorrelated to estimates of natural temperature (Grigg & Buckley, 2013) as a high number of terrestrial organisms are unlikely to evolve physiological resistance to increased heat (Kellermann et al., 2012;Hoffmann et al., 2013). In such cases, development of physiological resistances will be weakened.

Changes in energy reserves at fluctuating temperatures
Acclimation impacts the structure of lipid layers (Hazel, 1995), sugar or polyol amount (Hendrix & Salvucci, 1998) and metabolic rate (Hoffmann & Parsons, 1997): all of which can influence temperature resistance (Andersen et al., 2010). Whiteflies and aphids appear to be the first reported organisms that collected polyols in response to high temperatures (Hendrix & Salvucci, 1998). Sugars and polyols balance out and stabilize the natural structure of proteins, protecting them from warm denaturation (Back, Oakenfull & Smith, 1979).
The osmolyte compounds in aphids heated continuously for 3 h were less than those aphids exposed to three 1 h cycles of repeated high temperatures. Adaptations to surviving high temperatures, such as stress protein production and the synthesis of resources like glucose and trehalose (Hottiger et al., 1994;Jain & Roy, 2009). Accordingly, we anticipated that repeated high temperatures would result in the consumption of vital energy reserves.
Our results indicate that repeated high temperature exposure is physiologically costly for aphids compared to prolonged high temperature exposure, as there is a significantly lower amount of trehalose and triglyceride production after repeated high temperatures. Trehalose and proteins play important roles in stress responses (Parsell & Lindquist, 1993;Feder & Hofmann, 1999;Salvucci, Stecher & Henneberry, 2000;Jain & Roy, 2009;Smith et al., 2012). Induction of thermal tolerance by trehalose is also inferred from the fact that the level of trehalose is correlated with thermal tolerance (Hottiger et al., 1994): with this in mind, we measured the content of these two known compounds in M. persicae. Protein content was higher in aphids of the repeated high temperature treatment group, which has a well-defined role as a protective material at high temperatures (Jain & Roy, 2009) and this result is in agreement with previous studies (Huang, Chen & Kang, 2007;Tollarová-Borovanská, Lalouette & Koštál, 2009;Zhang et al., 2011). We hypothesise that in repeated high temperature exposure, protein production is triggered once the aphids are returned to their recovery temperature: a trigger that is not available to aphids exposed to prolonged periods of high temperature.
An unusual result in our study was that the amount of trehalose decreased in aphids exposed to repeated high temperatures compared to the aphids exposed to prolonged high temperatures. This contradicts the findings of previous studies assessing trehalose after exposure to high temperatures (Hottiger et al., 1994;Jain & Roy, 2009) but does support the findings of Teets et al. (2010). Trehalose may be used to produce an osmolyte, such as mannitol, in aphids which is important at high temperatures (Hendrix & Salvucci, 1998). Trehalose can also function as a reserve carbohydrate in others, which acts as a protectant. For example, it has been noted that trehalose accumulates under conditions of environmental stress, such as desiccation and is rapidly metabolized in rehydration. A reserve function could be inferred. Trehalose can also accumulate in response to other stresses, such as heat or osmotic shock (Newman, Ring & Colaco, 1993), for example Lalouette et al. (2007) demonstrated that trehalose can be changed to glycogen therefore is related to energy storage functions.
Triacylglycerols constitute a large part of the aphid lipid assemblage (Itoyama et al., 2000) and serve as a reservoir for fatty acids that can be used for energy production. Very large amounts of triacylglycerol can occur in aphids, comprising 20-30% of fresh body weight (Strong, 1963;Sutherland, 1968). According to expectations, we observed lower amount of triglyceride content after repeated high temperatures compared to the prolonged treatment aphids. One explanation is, these storage lipids are used as a source of metabolite energy for physiological processes: many insects use lipids as an energy source, and fatty acids are stored in the fat body in the form of triacylglycerol (Blacklock & Ryan, 1994;Itoyama et al., 2000). Since glycerol is known to maintain cells from hyperthermic cell death, induced thermal protection and warming protection may be applied by adjustment of either protein or membranes (Henle & Warters, 1982). Our results demonstrated that glycerol in aphids exposed to repeated extreme heat exposure (by the end of the third cycle) is higher than the levels in aphids exposed to prolonged thermal extremes; and this is in accordance with the role of this compound during heat stress (Benoit et al., 2007).
Aphids are very sensitive to acute changes in temperature rather than the duration of stress (Davis, Radcliffe & Ragsdale, 2006). We found significant changes in metabolite reserves after repeated high temperature exposure compared to the prolonged temperature exposure treatments with the same duration indicating that the number of extreme high temperature events is more important than the duration of extreme temperatures although the CT max of aphids was constant for both temperature regimes.

Impacts of repeated high temperature on metabolic rate of aphids
Respiration is the first process restricted in animals at low and high temperatures, and is connected to the limitation of blood circulation and ventilation (Portner, 2001). We observed no significant differences in metabolic rate 24 h after heating and this is in contrast to previous studies looking at metabolic rates at upper critical temperature (Klok, Sinclair & Chown, 2004;Boardman, Sorensen & Terblanche, 2013). Elevated standard metabolic rate after stressful conditions is a repair cost (Boardman, Sorensen & Terblanche, 2013). We measured active metabolic rate with increasing temperatures not resting metabolic rate or standard metabolic rate at 25 • C. In addition, we also found that there was a lower rate of water loss after heating: this may be due to aphids closing their spiracles to reduce water loss after heating events, and possibly a strategy for heat resistance.

OUTLOOK
To date, the key assessment of environmental stress tolerance in the aphid, M. persicae has been at low temperatures and in most known studies; the aphids have been exposed to a single stress event. In natural conditions, M. persicae is exposed to repeated bouts of high temperature punctuated by periods of recovery. Furthermore, because aphids are known to respond differently to consistent versus more 'normal' fluctuating temperatures, conclusions drawn from constant temperature studies may be unreliable. We suggest future experiments incorporate a repeated stress and recovery pattern into their methodologies to fully assess responses to extreme temperature exposure to reflect the natural growing conditions of this species of aphid and also, incorporate biological and fitness studies to physiological studies at the same time to reach comprehensive achievements in this filed.