Detection of Alzheimer’s disease by displacement field and machine learning

Aim. Alzheimer’s disease (AD) is a chronic neurodegenerative disease. Recently, computer scientists have developed various methods for early detection based on computer vision and machine learning techniques. Method. In this study, we proposed a novel AD detection method by displacement field (DF) estimation between a normal brain and an AD brain. The DF was treated as the AD-related features, reduced by principal component analysis (PCA), and finally fed into three classifiers: support vector machine (SVM), generalized eigenvalue proximal SVM (GEPSVM), and twin SVM (TSVM). The 10-fold cross validation repeated 50 times. Results. The results showed the “DF + PCA + TSVM” achieved the accuracy of 92.75 ± 1.77, sensitivity of 90.56 ± 1.15, specificity of 93.37 ± 2.05, and precision of 79.61 ± 2.21. This result is better than or comparable with not only the other proposed two methods, but also ten state-of-the-art methods. Besides, our method discovers the AD is related to following brain regions disclosed in recent publications: Angular Gyrus, Anterior Cingulate, Cingulate Gyrus, Culmen, Cuneus, Fusiform Gyrus, Inferior Frontal Gyrus, Inferior Occipital Gyrus, Inferior Parietal Lobule, Inferior Semi-Lunar Lobule, Inferior Temporal Gyrus, Insula, Lateral Ventricle, Lingual Gyrus, Medial Frontal Gyrus, Middle Frontal Gyrus, Middle Occipital Gyrus, Middle Temporal Gyrus, Paracentral Lobule, Parahippocampal Gyrus, Postcentral Gyrus, Posterior Cingulate, Precentral Gyrus, Precuneus, Sub-Gyral, Superior Parietal Lobule, Superior Temporal Gyrus, Supramarginal Gyrus, and Uncus. Conclusion. The displacement filed is effective in detection of AD and related brain-regions.


INTRODUCTION
Alzheimer's disease (AD) is an abnormal process of aging. AD is a special category of senile dementia (SD) which leads to short-term and long-term memory, thinking, and behavior (Dong et al., 2015a). Research on AD has attracted scholars from all over the world because of its importance and effect on the society. Symptoms of AD may become severe enough to interfere with daily life, and to death (Goh et al., 2014;Hahn et al., 2013). There is neither cure nor treatment for AD. In 2006, more than twenty million people in the world suffered from this disease (Murphy et al., 2011). AD is predicted to influence 1 in every 85 people worldwide about thirty years later, and more than forty percent of prevalent cases need high level of care (Brookmeyer et al., 2007).
Now that the earth is growing into an aging society, AD has caused heavier burdens on families and society than before (Esposito et al., 2013;Kantanen et al., 2014). In China, AD accounts for more than half of SD, which extracts a total economic loss of more than eighty billion yuan every year, and is responsible for nearly sixty billion yuan in healthcare costs every year (Song & Wang, 2010). In United States, healthcare on people with Alzheimer's disease currently costs roughly $100 billion per year and is predicted to cost $1 trillion per year by 2050 (Miller, Erlien & Piersol, 2012;Yuan, Machado & Arias-Carrion, 2014).
Nowadays, it is beneficial to develop early and accurate detection methods for AD, which is also necessary for the treatment and management for controlling the deterioration of AD (Zhang et al., 2015b). A 3D scan of the whole brain becomes acceptable and affordable with recent advances in neuroimaging technology (Zhang et al., 2015e;Zhang et al., 2014b), especially by the help of the most popular imaging technique: magnetic resonance imaging (MRI). With its high-resolution magnetic resonance (MR) images, the diagnostic accuracy of AD are greatly enhanced. MR images already play an essential role in detecting AD from normal elder controls (NC).
In this study, we proposed to employ displacement field (DF) to track the morphometry from normal brains to AD brains. The main advantage is that this method does not need to segment region-of-interest (ROI) beforehand, namely, it obtains the DF for the whole image. The classical level-set method was employed to estimate the DF.
This work is structured as follows: 'State-of-the-Art' provides the state-of-the-art. 'Materials and Methods' presents the materials and methodology. 'Experiments and Results' gives the experimental results. 'Discussion' discusses the results and the proposed method. 'Conclusions' concludes this work. The nomenclature in this work is listed in Table 7.

STATE-OF-THE-ART
In the past, most diagnosis work was carried out by measuring region of interest (ROI) of brain MR images, since researchers already know several typical AD-related regions and corresponding shape deformation (Anagnostopoulos et al., 2013;Kubota, Ushijima & Nishimura, 2006), such as the enlarged ventricles, the shrinkage of hippocampus (HC), and the shrinkage of cortex (Pennanen et al., 2004). Somehow, the ROI-based methods suffer from some shortcomings: (i) The ROI methods need a priori information and expert knowledge. (ii) The detection accuracy relies on the experiences of the interpreters (Yang et al., 2015). (iii) The mutual information among the voxels is difficult to implement (Lee, Park & Han, 2013;Xinyun, Wenlu & Xudong, 2011). (iv) We need to explore other potential regions that may be connected to AD (El-Dahshan et al., 2014). (v) Automatic segmentation of ROI is not feasible in practice, and examiners tend to segment the brain manually (Zhang et al., 2013).
Recently, a new type of methods, the "whole-brain analysis", gets popularity since it considers all voxels in the brain as a whole. It does not need to segment the brain beforehand, and it does not need any biomarker for classification task. The main disadvantage is the curse of dimensionality, which can be solved as the high-speed computers are very cheap nowadays (Álvarez et al., 2009). The whole-brain analysis heavily relies on pure computation, and it can be accomplished by only computer scientists, after physicians help to label the data as either AD or healthy. Generally, the whole-brain analysis treats the whole brain as a ROI, and it consists of two stages: feature extraction and classification. We reviewed more than 10 literatures, and analyzed them in detail.
Scholars have presented various methods to extract efficient features for AD and other types of pathological brain detection. 1 In addition, various classification models and 1 Some abbreviations are modified to avoid conflict within this paper. methods exist, nevertheless, only a few of them are suitable for MR images. El- Dahshan, Hosny & Salem (2010) extracted the approximation and detail coefficients of 3-level discrete wavelet transform (DWT). They used artificial neural network (ANN) and K-nearest neighbors (KNN) classifiers. Plant et al. (2010) used brain region cluster (BRC). They suggested to use information gain (IG) to evaluate the interestingness of a voxel, and applied clustering algorithm to identify groups of adjacent voxels with a high discriminatory power. They used support vector machine, Bayes statistics, and voting feature intervals (VFI), with the aim of pattern classification. Park (2012) employed manifold learning for classification. Their use of the first and second distance measure resulted in an 18% and 46% error rate for classifying between patients with AD and normal patients, respectively. Chaves et al. (2012) utilized large margin-based methodology for AD detection in SPECT and PET images. Their system yielded accuracy, sensitivity and specificity values of 90.67%, 88% and 93.33% (for PET) and 92.78%, 91.07% and 95.12% (for SPECT), respectively. Saritha, Joseph & Mathew (2013) was the first to use wavelet-entropy in pathological brain detection. They employed spider-web plots to reduce the size of feature space. They also used the probabilistic NN (PNN) for classification. Zhang et al. (2015a) suggested that removing spider-web-plot yielded the same classification performance. Savio & Grana (2013) presented a novel method that employed deformation-based morphometry (DBM) method. They tested five features, and found three features performed excellent as trace of Jacobian matrix (TJM), modulated GM (MGM), and Geodesic Anisotropy (GEODAN). Furthermore, they utilized Pearson's correlation (PEC), Welch's t-test (WTT), and Bhattacharyya distance (BD), to measure the significance of voxel site. Kalbkhani, Shayesteh & Zali-Vargahan (2013) modeled the detail coefficients of 2-level DWT by generalizing autoregressive conditional heteroscedasticity (GARCH) statistical model, and the parameters of GARCH model were considered as the primary feature vector. They tested the KNN and SVM models. Wang et al. (2014) proposed to utilize the undersampling (US) technique on a three-dimensional image. They used singular value decomposition (SVD) to select features. Finally, they combined kernel SVM (KSVM) with decision tree (KSVM-DT). Zhou et al. (2015) followed Saritha's method, and again employed wavelet-entropy for feature extraction. Naive Bayes classifier (NBC) was employed for abnormal brain detection. Harikumar & Kumar (2015) analyzed the performance of ANN, in terms of classification of medical images, using wavelets as feature extractor. Their classification accuracy achieved 96%. Zhang et al. (2015c) employed discrete wavelet packet transform (DWPT). They utilized Tsallis entropy to get wavelet packet entropy features. They introduced a generalized eigenvalue proximal support vector machine (GEPSVM). Nazir, Wahid & Khan (2015) suggested to use filters for the removal of noises, and extracted color moments as mean features. Finally, they achieved an overall accuracy of 91. 8%. Dong et al. (2015b) employed the eigen-brain (EB) to extract features, and then they employed Welch's t-Test (WTT) for the aim of reducing features. They proposed to use SVM with radial basis function (RBF). Damodharan & Raghavan (2015) combined tissue segmentation and neural network for brain tumor detection. Zhang et al. (2015f) proposed a novel classification system that implemented 3D discrete wavelet transform (3D-DWT) to extract wavelet coefficients the volumetric image. After reviewing the latest literature, two findings can be obtained: (1) The DWT based features are efficient. However, we presented a novel feature of displacement field, which is the first to be used in MR images. (2) SVMs were commonly used, compared with conventional decision tree, artificial neural network (Wang et al., 2015a;Zhang et al., 2015d), and other classifiers (Collins & Pape, 2011). Hence, we continue to use SVM. Besides, two variants of SVM are introduced in this study: generalized eigenvalue proximal SVM (GEPSVM) and twin SVM (TSVM), with the aim of augmenting the classification performance further.

Materials
The open dataset was downloaded from "Open Access Series of Imaging Studies (OASIS)" (Ardekani, Figarsky & Sidtis, 2013), which consists of 416 subjects aged from eighteen to ninety-six. All subjects are right-handed. Then, we merely selected 126 samples (28 ADs and 98 NCs) from the dataset. The demographic statuses are reported in Table 1. Following common convention, clinical dementia rating (CDR) was interpreted as the target (label). Note that subjects either with missing records or under sixty years old were removed.

Co-registration and brain-masking
All three-dimensional MR brain images of each subject were motion-corrected, and coregistered to generate an averaged three-dimensional image, with the aim of increas-ing signal-to-noise ratio. Afterwards, those three-dimensional images were spatially co-registered to the Talairach space and then were brain-masked. Figure 1 offers an example of the preprocessing on 3D images with resolution of 1 mm × 1 mm × 1.25 mm. The motion-correction procedure registered the images of three scans, and then generated an image in the original acquisition space with resampling to 1 mm × 1 mm × 1 mm. Finally, the averaged image was normalized to the Talairach coordinate space, and brain-extracted ( Fig. 1).

Key slice selection
Calculating the displacement field on the whole brain was time-consuming. Therefore, we introduced the "key-slice (KS) selection" method that picks up key slices containing structures indicative of AD from NC. The procedure was as follows: we established a criterion called "inter-class variance ( where k is the index of key slice, ∥ · ∥ 2 represents the l 2 -norm, µ A and µ N represents the mean of gray-level values of the kth slice of AD subjects and NC subjects, respectively, and v denotes the ICV. We select the KSs whose ICVs are larger than half of maximum ICV, with 10× undersampling factor (i.e., every ten slices). Besides, the direction of key slice can be either sagittal, or axial, or coronal. Scholars found images along coronal direction give a well-defined view compared to those along sagittal and axial directions. Figure 2 shows an image along coronal direction has an advantage because it covers 3 most important AD-related regions, which are treated as indicative of AD. These AD-related regions consist of the cerebral cortex (CC), the HC, and the ventricle. When sagittal or axial directions are used, two or even more slices are needed to cover those tissues. Therefore, we chose the coronal direction for key slice selection, in order to use less slices.

Shape registration
The shape registration consists of both global and local registrations. The global registration estimates the rigid parameters, which was accomplished in 'Co-registration and brain-masking' . Then, the local registration finds the "displacement field (DF)" between the moving and reference images (see Fig. 3).
The rigid registration is an essential preprocessing procedure before non-rigid registration. It can fix the deformation stemming from the position, move, and pose of patients. Then, the following non-rigid registration can reflect the shape deformation of diseases.
The problem of finding the non-rigid estimation can be thought of a motion estimation task between a normal brain (moving) and an AD brain (reference). Several types of solutions are available to solve this task, such as spline function based methods, phase-correlation methods, fluid methods, optical-flow methods, elastic methods, etc. Among the above methods, the first type is parametric, hence, this kind of solution needs to solve optimal spline-based function parameters (Petibon et al., 2013). The second type needs a mass of computation resources; it is difficult to determine the local search range, and it is impossible to guarantee to find global optimal points (Lu, Yang & Zhang, 2014). The other three methods are non-parametric. They find the DF by solving directly a predefined physical model using partial differential equation (PDE) (Yang et al., 2007). The level-set motion estimation method (Huang et al., 2014;Lee, Sandhu & Tannenbaum, 2013;Vandemeulebroucke et al., 2012) is a rather novel method, which is formed on the basis of the level set evolution theory. The moving image I 1 morphs iteratively along its gradient direction, till it deforms close to the given reference image I 2 . The displacement field is in the form of where V represents the displacement field, and I 1 (V) the deformed image of I 1 by V.
The above equation can be solved by iterative algorithms described in reference (Kodipaka et al., 2007).  Figure 4 gives an example of the displacement field between a glioma brain and a normal one. Figure 4A presents a moving image while Fig. 4B is a reference image. Their overlap is shown in Fig. 4C. Apparently, the reference image is larger than the moving image, and they are not quite a match. Figure 4D shows the result of rigid-registration, and Fig. 4E shows the two images now match well except the cortex, the temporal and occipital lobe.

Region detection
Step 1 Select a normal brain (I 1 ) and an AD brain (I 2 ) from the dataset Step 2 For each key slice k Implement level-set displacement-field estimation between I 1 and I 2 , and obtain the displacement field V. Move the points (x,y), that satisfies |V(x,y)| > 5, to the set R.

End
Step 3 Output R. Figure 4F shows the result of non-rigid registration. Compare to Fig. 4B, they closely match each other, and Fig. 4G also suggests a nearly-perfect match. The displacement field is shown in Fig. 4H, with two enlarged areas in Figs. 4I and 4J.
The values of displacement field are complex, i.e., the real part represents the displacement field along the horizontal direction, and the imaginary part along the vertical direction. Those values of Cartesian system are transformed into polar coordinate system, viz., the magnitude-direction spaces. Hence, the inputs to the classifiers are two fold in this study: (1) the directions of the displacement vector of the whole brain and (2) the magnitude of the displacement field of the whole brain. We did not use the sign of the displacement vector, which were commonly used in literature (Das Gupta & Tamadapu, 2013;Kodipaka et al., 2007). The reason is the direction field already contains the information of sign of the displacement.

Method of region detection
Here, we proposed a visual interpretation approach based on displacement field to detect regions R that can distinguish AD and NC.
Here | · | represents the magnitude, V(x,y) represents the displacement field at point of (x,y), T the threshold. From another point of view, Eq.
(3) means we preserve the points whose displacement magnitude is larger than T. In this work, T is assigned with a value of 5, which is defined empirically. A smaller threshold T may introduce more noises in the estimated displacement field; whereas, a larger threshold T will drop realistic deformation with short deformation magnitude. Hence, we believe the deformation with magnitude larger than 5 represents realistic deformation in the brain. In all, the visual interpretation is a three-step process (see Table 2).

Non-parallel support vector machine
Among all recent classifiers, the support vector machine (SVM) has gained popularity as the most excellent classifiers in small-size problem (Zhang & Wu, 2012). To further enhance the classification performance, two new variants of SVM were introduced:

Generalized eigenvalue proximal SVM
Original SVM has a limitation that two hyperplanes should be parallel . Mangasarian & Wild (2006) designed a generalized eigenvalue proximal SVM (GEPSVM). It drops the parallelism restrain on the two planes (remember the parallelism is necessary in original SVM), and requires each hyperplane should be as close as possible to one of the data sets and as far as possible from the other. The latest literature shows that GEPSVM yielded superior performance to canonical support vector machines in terms of sensitivity, specificity, precision, and accuracy (Khemchandani, Karpatne & Chandra, 2011;Shao et al., 2013). Suppose samples are from either class 1 (denote by symbol X 1 ) or class 2 (denoted by symbol X 2 ), respectively. The GEPSVM finds the two optimal nonparallel planes with the form of (w and b denotes the weight and bias of the classifier, respectively) To obtain the first plane, we deduce from Eq. (4) and get the following solution where o is a vector of ones with any possible dimension according to the context. Simplifying formula (5) gives We include the Tikhonov regularization term to decrease the norm of the variables U that corresponds to the first hyperplane in (4).
where t is a positive (or zero) Tikhonov factor. Formula (8) turns to the "Rayleigh Quotient (RQ)" in the following form of where P and Q are symmetric matrices in size of (p + 1) × (p + 1) Considering the stationarity and boundedness characteristics of RQ, the answer of (9) is deduced by figuring out a generalized eigenvalue problem (GEP) as here the global optimal result of (9) can be obtained at an eigenvector U 1 associated to the smallest eigenvalue λ min of formula (12). Therefore, w 1 and b 1 can be deduced from formula (6), and utilized to from the plane as written in formula (4). Afterwards, a similar optimization problem is generated that is analogous to (7) by exchanging the symbols of X 1 and X 2 . The eigenvector U 2 * corresponding to the smallest eigenvalue of the 2nd GEP will obtain the 2nd hyperplane approximate to samples of class 2.

Twin support vector machine
Jayadeva & Chandra (2007) provided a novel variant of standard SVM: the twin support vector machine (TSVM). The TSVM is similar to GEPSVM in the way that both obtain non-parallel hyperplanes. The difference lies in that GEPSVM and TSVM are formulated entirely differently. Each of the two quadratic programming (QP) problems in TSVM pair is formulated as a typical SVM. Reports have shown that TSVM is better than both SVM and GEPSVM (Nasiri, Charkari & Mozafari, 2014; Shao et al., 2014;Xu, Qi & Zhang, 2014). Mathematically, the TSVM is constructed by solving the two QP problems here c i (i = 1,2) are positive parameters, and o i (i = 1,2) is the same as in formula (5). By this means, the TSVM constructed two hyperplanes. The first term in equations of (13)(14) is the sum of squared distances from the hyperplane to one class. The second term is the sum of error variables. Therefore, minimizing Eqs. (13) and (14) will force the hyperplanes approximate to data of each class, and minimize the misclassification rate. Finally, the constraint requires the plane to be at a distance of more than 1 from data in the other class. Another advantage of TSVM is that its convergence rate is four times faster than conventional SVM (Jayadeva & Chandra, 2007).

Statistical analysis
Generalization error was obtained by K-fold CV, and K was set to 10 because of two reasons: (1) to balance between computational cost and reliable estimates, and (2) for fair comparison since the common convention is to set K with the value of 10 (Zhang et al., 2014a). For a 10-fold CV, the dataset is randomly divided into 10 mutually exclusively folds of nearly equal size. In each run, 9 subsets are used for training, and the rest for validation (see   Fig. 5). Above procedure repeats 10 runs, such that each subset is used once for validation. The 10 results over validation set are combined together along the diagonal blocks in Fig. 5, with the aim of producing an individual out-of-sample evaluation. The 10-fold cross validation repeated 50 times, viz., a 50×10-fold cross validation was implemented.

Evaluation
We used four indicators to measure which algorithm performed the best. Those four indicators consisted of sensitivity (recall), specificity, accuracy, and precision (Table 3). In this work, a correctly detected AD was treated as TP. After the 50×10-fold cross validation, the final evaluation results are written in the form of "mean ± standard deviation (SD)".

The whole proposed system
Remember that our aim contains two purposes. First, we need to develop a computeraided diagnosis (CAD) system and report its performance. Second, we need to locate Table 4 Pseudocode of proposed method.

Algorithm: proposed method
Step A Input the ground-truth imaging data together with their labels.
Step B Co-registration to Talairach Coordinate by Rigid Registration.
Step C Pick up the key-slices by ICV (more than half of maximum), with 10× undersampling factor.
Step D Produce displacement field for each key slice for each subject.
Step E Submit the displacement field to the classifiers.
Step F Report the classification performance based on a 50×10-fold cross validation.
Step G Report the AD-related regions with the points whose displacement magnitude is larger than five. discriminant voxels that can detect AD from NC subjects; and (ii) The pseudocode is listed in Table 4.

EXPERIMENTS AND RESULTS
The programs were developed in-house using MATLAB (Version 2015a; Natick, MA), and run on an IBM laptop with 3 GHz Intel i3 dual-processor and 8 GB random access memory (RAM).

Key-slice selection
The curve of "inter-class variance" versus coronal slice index is shown in Fig. 6A. Ten coronal slices, from 60 to 150 with increasing steps of 10, were selected. The reason is that their corresponding ICVs were all higher than half of the maximum value. Figures 6B-6C shows respectively the axial and sagittal view of the selected key-slices, where the red lines represent the key-slices. The results are coherent with our formal work (Dong et al., 2015b). Figure 7 shows the displacement field of an AD over the key-slices. Each column corresponds to a key slice. Here CI means coronal index. We show the moving image, the reference image, the overlap of both, the rigid registration, the overlap of rigid registration and the reference image, the non-rigid registration, the overlap of non-rigid registration and the reference image, and the displacement field for each key slice. From the row of displacement field, we can see how that a healthy brain is warped into an AD brain through the displacement fields (green arrows in the bottom row).

Classification comparison
The displacement fields were transformed into polar coordinate system, and their number was reduced by PCA as mentioned in the methodology section. Three classifiers were used: SVM, GEPSVM, and TSVM. Classification comparison results are provided in Table 5, together with the results of ten state-of-the-art methods.

Region detection
We implemented the AD-related region detection procedure to different AD subjects as 'Method of region detection' described. Figure 8 shows the related regions, which are labelled by green points. Some areas are slightly outside of the brain, such as Fig. 8A, this is because the algorithm may also consider the distortion of background. This does not influence following labelling procedure.

Area labeling
Talairach Daemon software was used to provide the anatomical label information. Table 6 shows the results, where BA represented Brodmann area. Note that some areas (such as cerebral tonsil, declive, and pyramis) move due to the expansion and shrinkage of neighboring areas, so they are not included in Table 6. We only pick up the areas with changed volume, which are judged manually.
Notwithstanding, some regions reported to be associated with AD are not interpreted by displacement field. Those areas include caudate nucleus (Montagne et al., 2015), claustrum (Pirone et al., 2015), lentiform nucleus (Dong et al., 2015b), subcallosal gyrus (Dong et al., Table 6 Discriminant areas with changed volume found by Talairach Daemon software. 112 Bangen et al. (2014) 2015b), and subthalamic nucleus (De Reuck et al., 2014). Why are those areas not detected by our DF method? The reasons are tri-fold: (1) We only preserved the displacement field with magnitude longer than 5. Reducing the value of 5 may include more potential regions and noises. A feasible solution is to reduce from 5 to 3 and meanwhile develop robust anti-noise method.

Regions # of voxels
(2) Some literature used advanced imaging modalities, such as MRSI and fMRI for metabolism detection and function analysis. Therefore, we may also include these imaging-modality techniques. (3) The key-slice selection procedure may miss important regions. Hence, we will try to reduce the slice separation, although it will increase the computation burden.
The advantages of displacement-field are two-fold. On one hand, it reaches excellent classification performance, which was comparable to latest approaches (Dong et al., 2015b;Plant et al., 2010;Savio & Grana, 2013;Wang et al., 2014). On the other hand, it can directly locate AD-related regions. The disadvantages of displacement field are: (i) The accuracy of displacement field estimation relies on the accuracy of rigid registration, which is a necessary pre-processing (see Fig. 3). (ii) The estimated DF may exist in the background (see Fig. 8A), which needs to be removed by masking technique.

CONCLUSIONS
The contributions of this study consist of four points: (i) We proposed to use displacement field in the application of Alzheimer's disease detection, and we proved its effectiveness.
(ii) SVM and its two variants (GEPSVM and TSVM) were tested, and we proved TSVM performed better than SVM and GEPSVM. (iii) The proposed system achieved comparable sensitivity, specificity, and accuracy with ten state-of-the-art algorithms. (iv) The proposed computer-aided diagnosis (CAD) system can locate AD-related regions in the brain, which complies with 28 recent peer-reviewed articles. Future research direction shall center in following aspects. First, the displacement field will be generalized to 3D, so key-slice selection can be discarded. Second, we shall try to embed kernels to classifiers. Third, spectroscopy method, like extracting the quantity of Docosahexaenoic Acid (DHA) (Tan et al.), may help to increase the classification accuracy. Forth, the proposed approach might be helpful in identifying brain mechanisms underlying endogenously defensive mechanism to neuroinjury and neurodegeneration (Yuan & Hou, 2015). Fifth, the least square technique (Hamedi, Salleh & Noor, 2015) may be applied to SVM and its variants, in order to further reduce training time. Sixth, the swarm-intelligence algorithm will be introduced to help to enhance the algorithm performance (Wang et al., 2015b). Finally, the stress field calculates the stress at every point within a particular region (Petit et al., 2015). It will be tested in future research.  BK20150983). The authors obtained the OASIS dataset made possible by NIH grants P50AG05681, P01 AG03991, R01 AG021910, P50 MH071616, U24 RR021382 and R01 MH56584. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Grant Disclosures
The following grant information was disclosed by the authors: NSFC: 610011024, 61273243, 51407095. Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).