Rumen bacterial community profile and fermentation in Barki sheep fed olive cake and date palm byproducts

Rumen bacteria make the greatest contribution to rumen fermentation that enables the host animal to utilize the ingested feeds. Agro-industrial byproducts (AIP) such as olive cake (OC) and date palm byproducts (discarded dates (DD), and date palm fronds (DPF)) represent a practical solution to the deficiency in common feed resources. In this study, thirty-six growing Barki lambs were divided into three groups to evaluate the effect of untraditional diets including the AIP on the growth performance. Subsequently, nine adult Barki rams were used to evaluate the effect of experimental diets on rumen fermentation and rumen bacteria. Three rations were used: common concentrate mixture (S1), common untraditional concentrate mixture including OC and DD (S2), and the same concentrate mixture in S2 supplemented with roughage as DPF enriched with 15% molasses (S3). The animals in S2 group showed higher dry matter intake (DMI) and lower relative growth rate (RGR) as compared to the animals in S1 group. However, the animals in S3 group were the lowest in DMI but achieved RGR by about 87.6% of that in the S1 group. Rumen pH, acetic and butyric acids were more prevalent in animals of S3 group and rumen ammonia (NH3-N), total volatile fatty acids (TVFA), propionic acid were higher in S1. Rumen enzymes activities were higher in S1 group followed by S3 and S2. The bacterial population was more prevalent in S1 and microbial diversity was higher in the S3 group. Principal coordinate analysis revealed clusters associated with diet type and the relative abundance of bacteria varied between sheep groups. The bacterial community was dominated by phylum Bacteroidetes and Firmicutes; whereas, Prevotella, Ruminococcus, and Butyrivibrio were the dominant genera. Results indicate that diet S3 supplemented by OC, DD, and DPF could replace the conventional feed mixture.


INTRODUCTION
The dramatic increase in animal feed prices encouraged nutritionists to search for cheaper alternatives to the traditional feedstuffs (Al-Dabeeb, 2005). Locally available byproducts functional genes, metabolic pathway, rumen metabolites, and animal performance (Denman, Morgavi & McSweeney, 2018;Li et al., 2019).
The Barki sheep is a small breed that has white wool and a brown neck and is distributed in the desert in the Mediterranean zone; also, it has long thin legs that allow travel for long distances in search of grass (Elshazly & Youngs, 2019). This breed is well adapted to survive in harsh conditions, including poor feeding, heat stress, and diseases (Abdel-Moneim et al., 2009). The Barki sheep is the main sheep breed in Egypt's desert; it provides meat and milk under desert conditions; thus, it represents an important food supply in Egypt (Abousoliman et al., 2020). Despite the economic importance of Bakri sheep in Egypt, the rumen microbiome did not receive attention in comparison to other domesticated ruminants. Ruminant production in the Mediterranean countries is challenged by poor quality and scarcity of pastures especially during the drought periods besides the increase in cereal prices (Elshazly & Youngs, 2019). Therefore, it is important to develop new strategies to improve animal efficiency using locally available resources (Romero-Huelva, Ramos-Morales & Molina-Alcaide, 2012). Feeding systems that depend on OC, DD, and DPF are common in arid countries including Egypt; besides, several studies interested in the effect of inclusion of olive cake and date palm byproducts on animal performance and rumen fermentation (Estaún et al., 2014;Khattab & Tawab, 2018). However, there is still limited information on the impact of these residues on the rumen microbiome. Mannelli et al. (2018) incorporated olive cake into the diet of lactating ewes and observed that the relative abundances of some bacterial taxa have been affected. On the other hand, no data were available on the effect of date palm byproducts on rumen bacteria. Additionally, no previous reports studied the effect of rations including OC, DD, and DPF on animal performance, rumen fermentation, and rumen bacteria. Therefore, the objective of this study was to evaluate the effect of common untraditional feeding systems that based on OC, DD, and DPF on the growth performance, rumen fermentation, and rumen bacteria community of Barki sheep.

Growth trial
Growth trial was conducted at a commercial private farm in Matrouh government as a part of project "The Executive Project for Breeding and Production Systems Development of Camel and Small Ruminant in the Northwest Coast of the Arab Republic of Egypt" that was funded by the Arab Center for Studies of Arid Zones and Arid Lands (ACSAD). The project was conducted and supervised by the Desert Research center, Egypt. Thirty-six growing male Barki lambs with an average body weight of 29.65 ± 0.29 kg (mean ± SE) were randomly divided into equal three groups and housed in 12 wellventilated shaded pens as three replicates in each group (three lambs/pen). The animals were assigned to one of three treatments and received ad lib diets for 75 days and free access to drinking water was provided. The experimental rations were: control ration or common concentrate mixture (S1); non-traditional concentrate mixture including 10% olive cake (OC) and 60% discarded date palm (DD) (S2); the same concentrate ration in S2 supplemented with ground date palm fronds (DPF) enriched with 15% molasses as roughage (S3). The physical composition of the experimental rations is presented in Table 1. Lambs were weighed biweekly until the end of the experiment. At the end of the growth trail, the animals were left on the farm without euthanizing.

Digestibility and rumen fermentation trials
Nutrients digestibility and rumen fermentation trials were conducted at Maryout Research station, Desert Research center, Egypt. Nine adult Barki rams with an average body weight of 55.10 ± 1.47 kg (Mean ± SE) were assigned into three groups (three animals/ treatment) to evaluate the impact of experimental diets on the digestibility of DM, CP, and NDF as well as investigation of rumen fermentation and composition of rumen bacterial community.
Animals were adapted for the diet in individual metabolic cages for 15 days, followed by 7 days as a collection period. Drinking water was offered free before the collection period and two times during the collection period. Daily collected feces were subsampled and dried at 70 C for 48 h, then grounded and conserved until analysis. On day 22, rumen liquid was collected using a stomach tube before the morning feeding and the pH of rumen fluid was immediately recorded using a digital pH meter (WPA CD70). Rumen samples were used for the analyses of rumen ammonia and VFA, DNA extraction, and lignocellulolytic enzymes assays.
This study, including the growth trail and digestibility trail, was conducted under guidelines set by the Department of Animal and Poultry Production, Desert Research Center, Egypt. Moreover, the project was approved by the Institutional Animal Care and Use Committee, Faculty of Veterinary Medicine, University of Sadat City, Egypt (Reference: VUSC00008). All methods were performed in compliance with the ARRIVE guidelines. In addition, the project does not include euthanasia of the experimental animals. The sample size was decided based on the availability of animals that are similar in age, weight, physiological stage.

Chemical analyses
The experimental diets were analyzed for dry matter, crude protein (CP), and crude fiber (CF) according to AOAC (1997). Neutral detergent fiber (NDF) and acid detergent fiber ADF contents were determined by the method of Van Soest, Robertson & Lewis (1991) without sodium sulfite. Dry fecal samples were analyzed for CP and NDF. Moreover, the rumen ammonia and total VFA concentrations were determined by steam distillation in a Kjeldahl distillation according to the methods of Annison (1954) and AOAC (1997), respectively. In addition, individual VFAs were measured using high-performance liquid chromatography (HPLC) (Weimer, Shi & Odt, 1991).
Additionally, cellulase and xylanase activities were determined by quantifying the released reducing sugars by the 3, 5-dinitrosalicylic acid (DNS) (Ghose, 1987;Bailey, Biely & Poutanen, 1992). Xylanase was measured as endo-xylanase that was defined as the amount of enzyme that releases 1 mmol of xylose per ml in a minute. Cellulase was quantified as a unit of endo-β-1,4-glucanase that is defined as the amount of enzyme that could hydrolyze filter paper and release 1 µmol of glucose within 1 min of reaction.

DNA extraction, PCR amplification, and sequencing
One milliliter of rumen fluid was centrifuged at 13,000 rpm and the precipitated pellets were used for DNA extraction by i-genomic Stool DNA Extraction Mini Kit (iNtRON Biotechnology, Inc., Seongnam-si, South Korea) according to the manufacturer's instructions. DNA was then eluted in 50 µL elution buffer and DNA quality and quantity were verified using agar gel electrophoresis and Nanodrop spectrophotometer (Thermo Fisher Scientific, Madison, WI, USA). DNA Amplicon libraries targeting the V4-V5 region of the 16S rRNA bacterial 16S ribosomal DNA gene were generated by PCR amplification using primers 515F (5′-GTGYCAGCMGCCGCGGTAA-3′) and 926R (5′-CCGYCAATTYMTTTRAGTTT-3′) (Walters et al., 2015). PCR amplification was conducted under the following conditions: 94 C for 3 min; 35 cycles of 94 C for 45 s, 50 C for 60 s, and 72 C for 90 s; and 72 C for 10 min. PCR products' purification, preparation for sequencing using Illumina MiSeq system were conducted according to the protocol described by Comeau, Douglas & Langille (2017) in Integrated Microbiome Resource (Dalhousie University, Canada).

Quantitative real-time PCR
Real-time PCR was conducted to determine the total bacterial 16S rRNA copy number in the rumen fluid. Standards were generated using dilutions of purified genomic DNA from Prevotella sp, Ruminococcus albus, Butyrivibrio hungatei purchased from DSMZ (Braunschweig, Germany). Dilution series of the standards ranging from 10 1 to 10 6 copies of the 16S rRNA gene were used. The qPCR was performed using the Applied Biosystems StepOne system (Applied Biosystems, Foster City, CA, USA). The bacterial specific primers F (5′-CGGCAACGAGCGCAACCC-3′) and R (5′-CCA TTGTAGCACGTGTGTAGCC-3′) (Denman & McSweeney, 2006) were applied to amplify DNA samples and diluted standards. The 10-mL reaction consisted of 1 mL genomic DNA, 1 mL of each primer, and 7 mL SYBER Green qPCR-master mix (iNtRON Biotechnology, Inc., Seongnam-si, South Korea). The PCR conditions were as follows: 40 cycles of 95 C for 15 s, and 60 C for 60 s. The linear relationship between the threshold amplification (Ct) and the logarithm of 16S rDNA copy numbers of the standards was used to calculate the copy numbers of rumen bacteria per mL of DNA.

Bioinformatics analyses
The bioinformatics analyses of the paired-end (PE) Illumina raw sequences were processed in R (version 3.5.2) using DADA2 (version 1.11.3) (Callahan et al., 2016). Briefly, reads will be denoised, dereplicated and filtered for chimeras to generate Amplicon Sequence Variants (ASVs). Taxonomic assignment of sequence variants was compared using the latest SILVA reference database SILVA. The resulting ASV table was normalized and subsequently used to perform downstream analyses, including the computing of alpha and beta diversity metrics and taxonomic summaries.

Statistical analysis
The statistical analyses were conducted using the IBM SPSS version 20 software (SPSS, 1999). The differences in feed intake, relative growth rate, rumen fermentation parameters, rumen enzymes, bacterial copy number, microbial diversity, and relative abundance of bacterial phyla and genera were performed using one-way ANOVA based on a post hoc Duncan test. For all statistical tests, p-values < 0.05 were considered significant. The results of relative abundance of bacteria were tested for normality using Shapiro-Wilk test and non-normal values were then arcsine transformed. All the sequences were deposited to the sequence read archive (SRA) under the accession number: PRJNA744569.

Chemical Composition of the experimental diets
The composition and chemical analysis of animal diets are presented in Tables 1 and 2. The results indicated that olive cake and discarded date palm represented 70% of the untraditional concentrates mixture (S2). Moreover, the traditional concentrates mixture (S1) contained higher content of CP and NDF compared with S2. Whereas, S2 contained higher content of DM, CF, and ADF compared with S1.

Performance of growing lambs
The results of dry matter intake (DMI), organic matter intake (OMI), and the relative growth rate (RGR) are presented in Table 3. The differences in DMI and RGR were significant among groups. Animal group S2 consumed the highest total DMI followed by S1 and S3, respectively. Both S2 and S3 groups showed a comparable RGR value but they are lower than that revealed in the S1 group.

Digestibility of nutrients in adult rams
Sheep group S2 showed the highest total DMI followed by S3 and S1, respectively ( Table 3). The untraditional diets (S2 and S3) showed lower values for CP and NDF digestibility as compared to the traditional diet (S1).

Rumen fermentation parameters and lignocellulolytic enzymes
The effect of ration type on rumen fermentation parameters and enzymes assays are presented in Table 4. The ration S3 that containing DPF resulted in a higher rumen pH value followed by S2 and then S1; and rumen ammonia concentration followed the same trend. Both S2 and S3 showed significantly lower total VFA concentration but higher in acetic and butyric fractions as compared with S1. However, the ration S1 was superior in propionic acid fraction. S1 group showed higher values for rumen xylanase and cellulase production as compared with the other two groups but still without significant differences (Table 4). The bacterial population (Log10 of 16S rDNA copies) was significantly affected by the experimental rations. The copy number was higher in the S1 group followed by S3 and then S2, respectively (Table 4).

Rumen bacterial diversity analysis
Following quality filtering, merging and removal of chimeric sequences, the sequencing of the V4 region on 16S rDNA in rumen samples from nine sheep resulted in 196999 high-quality sequence reads with an average of 21,888 ± 1,113 reads per animal (Mean ± Standard error; SE) ( Table 5). Alpha diversity metrics were used to estimate the similarity in the bacterial community among sheep groups. The bacterial richness was estimated using Chao and ACE indices. Moreover, the bacterial diversity was determined using Invsimpon and Shannon indices. The sheep group fed S3 diet showed higher ASVs number and alpha diversity indices compared to S2 and S1 groups with a significant difference in Shannon index. The principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity (Fig. 1) used to estimate beta diversity of bacterial communities Table 4 Rumen fermentation parameters and enzymes activites. Rumen pH, ammonia nitrogen (NH3-N), total volatile fatty acid (VFA) concentration and VFA proportions, cellulase and xylanase enzymes, and bacterial population (Log10 copies/mL DNA) in the rumen of Barki sheep under investigation (Mean ± SE).

Item
Feeding within sheep groups confirmed that sheep groups were separated distinctly from each other.

Spirochaetes
Proteobacteria Firmicutes Bacteroidetes Figure 2 The relative abundance of dominant bacterial phyla. The relative abundance of dominant bacterial phyla in the rumen of three sheep groups (S1, S2, S3). Full-size  DOI: 10.7717/peerj.12447/ fig-2 was dominated by RC9_ gut group. In addition, family Prevotellaceae was higher in S1 group with a significant difference and most of the family's reads were assigned to genus Prevotella and uncultured Prevotellaceae. Phylum Firmicutes was the second largest phylum in sheep rumen, it was significantly higher in the S1 group (22.7%), followed by S2 (22.1%), and S3 (15%) ( Table 6 and Fig. 2). This phylum was dominated by five families, including Veillonellaceae, Erysipelotrichaceae, Ruminococcaceae, Lachnospiraceae, and Family_XIII. While group S2 showed the highest representation of family Lachnospiracea, the sheep group S1 diet showed the highest representation of Veillonellaceae, Erysipelotrichaceae, and Ruminococcaceae. Furthermore, Family_XIII was higher in S3. In addition, phylum Firmicutes was dominated by five genera, including Selenomonas, Ruminococcus, Butyrivibrio, Megasphaera, and Sharpea. These genera were also affected by diet type; Selenomonas was higher in S1group followed by S3 and S2, respectively. Butyrivibrio was higher in S2 followed by S3 and S1 respectively (Table 6 and Fig. 2).

Chemical composition of diets
Physical and chemical characteristics of animal diet are the main driver of modulations in the rumen microbiome (Denman, Morgavi & McSweeney, 2018); therefore, understanding the rumen microbiome could lead to developing strategies to improve animal productivity (Wirth et al., 2018). Agricultural byproducts provide suitable alternatives to the common feedstuffs in the arid countries (Mioč et al., 2007;Awawdeh & Obeidat, 2013;Allaoui et al., 2018). However, those byproducts should be evaluated on the level of their effect on animal performance and the rumen ecosystem (Alnaimy et al., 2017). To the best of our knowledge, this study is the first to report the effect of using untraditional diets that contain a combination of olive cake and date palm byproducts (discarded date or dates palm fronds) in sheep feeding on animal performance, rumen fermentation, and rumen bacteria ( Table 1). The common concentrate feed mixture is a starchy mixture and has low-fiber content and high content of crude protein, energy, and soluble carbohydrates (Carberry et al., 2012). At the same time, olive cake and date palm fronds are described as low-quality feedstuffs with high contents of lignocelluloses and low contents of protein and energy (Awawdeh & Obeidat, 2013;Boufennara et al., 2016;García-Rodríguez et al., 2020). Furthermore, the discarded dates are considered as an energy source due to high soluble carbohydrates and contain low crude protein (Al-Dabeeb, 2005;Boufennara et al., 2016;Allaoui et al., 2018).

Animals' performance
In this study, the highest DMI was obtained by group S2, which fed feed mixture contains OC, and DD; while the lowest DMI was obtained by group S3, which fed feed mixture contains OC, and DD plus DPF (Table 3), this finding in line with a study by Rajabi et al. (2017), when discarded dates included in sheep 's diet. De Visser et al. (1998) indicated that the inclusion of the late cut grass with high-fiber content decreased the DMI. The higher DMI in S2 could be attributed to the palatability of diet contains DD or rapid passage rate Iqbal et al., 2019).
Untraditional CFM diet alone (S2) or supplemented with date palm fronds (S3) resulted in low relative growth rate (54.43 vs. 57.78%, respectively) but the highest value was recorded for the S1 group (66.02%) that fed common feed mixture (Table 3). The decline in growth rate in S2 and S3 diets could be attributed to low protein and high fiber content compared to the S1 diet (Kewan, 2013;Allaoui et al., 2018). This finding is similar to previous studies that indicated that the inclusion of the olive cake or discarded dates declined the growth performance (Al-Dabeeb, 2005;Mioč et al., 2007;Allaoui et al., 2018). In addition, Abo Omar, Daya & Ghaleb, 2012 mentioned that the inclusion of olive cake into Awassi lambs' diet did not affect the performance adversely. Mahgoub et al. (2007), reported that sheep fed date palm fronds gained less weight than those fed rhodes grass hay; which could be due to higher fiber content and lower protein content in palm fronds (Khattab & Tawab, 2018).
The decline in growth rate in S2 and S3 diets groups could be attributed to lower dietary protein and high fiber contents compared to the S1 diet (Al-Dabeeb, 2005;Allaoui et al., 2018). More studies mentioned that incorporation the olive cake and date palm byproducts in lamb diets declined the digestibility of DM, OM, CP, NFE, and CF (Al-Dabeeb, 2005;García-Rodríguez et al., 2020), which support our results and could be attributed to high fiber and phenolic compounds that limit the availability of nutrients and affect the rumen microbiota negatively (Mioč et al., 2007;Awawdeh & Obeidat, 2013;Boufennara et al., 2016;Djamila & Rabah, 2016). Therefore, animals fed on such byproducts will require protein supplementation (Allaoui et al., 2018); also, feeding the animals on crude byproducts should be avoided (Mioč et al., 2007).
Lower digestibility of CP for both S2 and S3 than S1 (Table 3) might be due to higher condensed tannins in dates, which bind with protein and reduced its utilization. These results are similar to the findings that reported by Kewan (2013) who found that CP digestibility was 71.49 and 77.38 for lambs fed concentrate feed mixture with complete replacement of corn grains (27.5% of the TMR) by discarded dates and control group, respectively. In addition, lower digestibility of NDF for both S2 and S3 than S1 might be due to the soluble carbohydrate in the dates which has commonly been associated with rapid fermentation and subsequent depression of ruminal pH. This finding was confirmed by Khattab, Abdel-Wahed & Kewan (2013) who found a linear decrease of NDF digestibly by increasing the dates inclusion rate.

Rumen fermentation activity
The improvement in rumen pH in S2 and S3 compared to S1 diet (Table 4) was also indicated in other studies on olive cake (Awawdeh & Obeidat, 2013;García-Rodríguez et al., 2020). In addition, the incorporation of the DPF in the S3 diet has increased the rumen pH compared to the other two groups. Khattab & Tawab (2018) reported that the inclusion of DPF in sheep's diet kept the rumen pH higher than sheep fed clover hay. The decline in rumen pH has a negative consequences on rumen fermentation (Dijkstra et al., 2012), which could be a positive point for using S3 diets in sheep feeding. The concentrate feed mixtures consist of rapidly fermentable carbohydrate that encourages the VFA production that declines the rumen pH; in contrast, the higher content of ADF and NDF in S3 diets has neutralized the rumen pH (Asadollahi et al., 2016). The higher concentration of ammonia in S1 is reflected by the higher dietary protein compared to other diets (Hamchara et al., 2018;Khattab & Tawab, 2018;García-Rodríguez et al., 2020). Previous studies showed that incorporation of the DD in the animal diet decreased the protein content, rumen ammonia, and VFA; and did not affect VFA composition (Rajabi et al., 2017;. Another explanation for the linear decrease of ammonia is soluble sugars in the S2 and S3 capture more degradable nitrogen for microbial protein synthesis than the starch in S1 . The inclusion of OC, DD, and DPF in the diet changed the proportions of individual VFAs. Acetic and butyric acids were increased a long with a decrease in propionic acid (Table 4); this finding is in agreement with previous studies (De Visser et al., 1998;Pallara et al., 2014;Asadollahi et al., 2016;García-Rodríguez et al., 2020) that revealed that higher dietary fiber stimulates the production of acetic acid and decrease the propionic acid due to increasing the activity of fibrolytic bacteria. The increase of butyrate in S2 and S3 groups was also obtained in previous studies on DD in sheep diets . Analyzing the effect of the feeding system on VFA production in the rumen is important as VFAs provide the animal with two-third of the energy supply of the ruminant (De Visser et al., 1998). Consequently, the production of meat and milk could be affected; for instance, the reduction in acetic to propionic ratio has a depressing effect on milk fat production (Sutton et al., 2003).
The activities of rumen enzymes and VFA production are the reflection of rumen microbial groups involved in rumen fermentation (Kamra, Agarwal & McAllister, 2010;Latif et al., 2014). According to a study by Raghuvansi et al. (2007), the increase in the production of fibrolytic enzymes could be attributed to the higher numbers of fibrolytic bacteria. Our results indicated that group S1 showed higher enzymes activities (cellulase and xylanase), and a higher bacterial population followed by S3 (Table 4); this finding could be attributed to the presence of rapidly degradable carbohydrates and degradable nitrogen in S1 groups that stimulated bacterial growth (Raghuvansi et al., 2007;Azizi-Shotorkhoft et al., 2018). Moreover, higher cellulase production and bacterial population in S3 compared to S2 could be explained by the presence of molasses in the diet and higher content of ADF, which agrees with the results of Azizi-Shotorkhoft et al. (2018) who indicated that the inclusion of molasses increased the cellulolytic activities. On the other hand, Khattab & Tawab (2018) reported that the inclusion of palm fronds in the animal diet reduced microbial protein due to low protein content that depresses microbial growth. The information about the effect of OC, DD, and DPF on rumen enzymes and the bacterial population is limited; an In vitro study conducted by García-Rodríguez et al. (2020) reported an increment in the bacterial density and reduction of the microbial protein by incorporation olive cake into the animal diet. Moreover, Dahlan (2000) showed that goat fed oil palm fronds produced more ammonia, TVFA, acetic acid, cellulase and xylanase compared to goat fed rice straw, which supports our findings. Sheikh et al.
(2019) revealed that feed digestibility and microbial activity were improved when degradable protein was added to the diet, which could explain the higher bacterial population and VFA production in S1.

Diversity and composition of rumen bacteria
Inclusion of the OC, DD, and DPF to the concentrate mixture has changed the diversity and composition of rumen bacteria (Tables 5 and 6 and Figs. 1 and 2), which is in agreement with previous studies on the olive cake (Pallara et al., 2014;Mannelli et al., 2018;García-Rodríguez et al., 2020). Mannelli et al. (2018) reported that rumen microbiota is highly affected by dietary composition especially the chemical compounds that have antimicrobial activities such as polyphenols observed in byproducts. At the same time, no available data on the effect of discarded dates and date palm fronds on the rumen microbial populations. The higher ASVs were linked with sheep group fed S3 diet that contains concentrate mixture plus forage, which is similar to findings obtained by Petri et al. (2012) and Rabee et al. (2020a). In this study, phylum Bacteroidetes and Firmicutes dominated the bacterial community; this finding was also reported in the rumen of lactating ewes fed olive cake (Mannelli et al., 2018), cattle (Petri et al., 2013), and camel (Rabee et al., 2020a).
Firmicutes were dominated by the family Ruminococcaceae and Lachnospiraceae (Tables 6 and 7), which is in agreement with results obtained by Rabee et al. (2020a). Members of family Ruminococcaceae degrade the hemicellulose, pectin, and cellulose present in the plant cell wall (Pettipher & Latham, 1979), which could explain the high representation of this family in S1 group that fed mixture with high-NDF content and group S3 that fed mixture with high-ADF and NDF contents. The decline in the relative abundance of Ruminococcaceae in S3 compared to S1 could be attributed to the presence of phenolic compounds in olive cake and date palm byproducts that affect fibrolytic bacteria (Khattab & Tawab, 2018;Mannelli et al., 2018). According to Moumen et al. (2007), the optimal fermentation of lignocellulosic byproducts could be achieved by the addition of nitrogen and soluble carbohydrates to animal diets, which enhance rumen fermentation; this might illustrate the higher abundance of Ruminococcaceae in S1.
Family Lachnospiraceae was dominated by genus Butyrivibrio that is cellulolytic bacteria and was overrepresented in S2 and S3 groups that fed diets with higher ADF that favors the cellulolytic bacteria (Pitta et al., 2014;Liu et al., 2017). The decline in the abundance of this genus in S1 could be a result of lower fiber content (Mannelli et al., 2018). In addition, the genus Butyrivibrio is involved in the production of butyrate that explains the higher amount of butyrate in S2 and S3 (Bharanidharan et al., 2018).
Otherwise, the decline of Butyrivibrio in S3 compared to S2 could be attributed to the presence of DPF that has higher polyphenol content (Boufennara et al., 2016;Mannelli et al., 2018). Anaerovibrio within family Veillonellaceae, has an essential role in the lipid metabolism and biohydrogenation in the rumen, this process is highly associated with concentrate content in animal diet that enhances the lipolysis (Prins et al., 1975).
Consequently, the presence of this genus in the S1 and S2 groups is explained. Moreover, the disappearance of this genus from the S3 group could be linked to high fiber and polyphenols content due to the inclusion of date palm fronds that rich in both fiber and polyphenols (Mannelli et al., 2018;Boufennara et al., 2016). Thus, the polyphenols have an adverse effect on some of the bacterial genera involved in biohydrogenation in the rumen (Frutos et al., 2004;He, Lv & Yao, 2007). Consequently, the quality of animal products (milk and meat) could be improved by the incorporation of polyphenols in ruminant diets (Pallara et al., 2014). Vasta et al. (2010) reported that the inclusion of polyphenols in ruminant diets affects rumen metabolism, decreasing dietary protein degradation and fatty acid biohydrogenation through targeting specific groups of microorganisms. At the phylum level, Bacteroidetes dominated the bacterial community in sheep groups, and its relative abundance varied between groups (Table 6 and Fig. 2). This phylum was dominated by uncultured bacteria that are specialized in lignocellulose degradation (Naas et al., 2014), which explains the higher abundance in the S3 group. In addition, this phylum was dominated by Prevotella and RC9_gut_group, which is in agreement with a finding of Fouts et al. (2012) on cows. Prevotella can degrade starch, hemicelluloses, pectin and could produce propionate and xylanase in the rumen (Russell & Rychlik, 2001). Consequently, the overrepresentation of this genus in the S1 group is expected and explains the higher propionic acid and xylanase enzymes in the S1 group. RC9_gut_group was previously observed in camel and Rhinoceros gut and could have a role in the degradation of lignocellulose, which might illustrate its higher representation in group S3 (Mackenzie et al., 2015;Rabee et al., 2020b). Furthermore, phylum Fibrobacteres was more abundant in group S3 (Table 7), which is an expected trend as this phylum is the major cellulolytic bacteria in the rumen (Ransom-Jones et al., 2012;Petri et al., 2012). Phylum Elusimicrobia and Verrucomicrobia were found higher in the S3 group; some members of these phyla have a role in fiber degradation, which supports our results (Hou et al., 2008;Herlemann et al., 2009;Lee et al., 2009). The members of Proteobacteria have a role in protein degradation (Liu et al., 2017).

CONCLUSIONS
Olive cake and date palm byproducts are available feed resources that could replace common concentrate feed mixture especially in case of limited availability or high price. Inclusion of OC, DD, and DPF affected animal performance and rumen fermentation parameters as well as microbial diversity and the relative abundance of rumen bacteria. Additionally, lignocellulolytic bacteria showed an increase in their relative abundance in the rumen of sheep fed byproducts. The composition of the rumen bacterial community in Barki sheep is similar to other ruminant animals.
Mebarek Lamara conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers): The Institutional Animal Care and Use Committee, Faculty of Veterinary Medicine, University of Sadat City, Egypt approved the study (Approval reference number: VUSC00008).

Data Availability
The following information was supplied regarding data availability: