| 玉米BBX基因家族鉴定及表达分析 |
| 点此下载全文 |
| 引用本文：赵长江,徐尚缘,都梦翔,宋巨奇,徐晶宇,杨克军,李佐同.玉米BBX基因家族鉴定及表达分析[J].西北农业学报,2022,30(6):677~689 |
| DOI:10.7606/j.issn.1004-1389.2022.06.001 |
| 摘要点击次数: 615 |
| 全文下载次数: 363 |
|
| 基金项目:国家自然科学基金（31701328）；黑龙江省自然科学基金重点项目（ZD2020C007）。 |
|
| 中文摘要:BBX（B-box）是一类光温响应转录因子家族参与植物光形态建成及逆境响应。为揭示ZmBBX家族基因功能，采用生物信息学方法在玉米基因组水平对该基因家族进行鉴定，并对其基本理化性质、基因结构、系统进化关系、启动子顺式作用元件和基因表达谱进行分析。ZmBBX基因家族共有34个成员，划分为5个亚族，编码蛋白氨基酸介于142~498 aa，预测大多数成员定位于细胞核内。ZmBBX基因启动子存在大量的光响应元件、胁迫响应元件、激素应答元件和生长发育相关的顺式作用元件。全生育期组织表达谱发现， ZmBBX7、 ZmBBX27和 ZmBBX28在玉米的全生育期均高表达， ZmBBX3、 ZmBBX5、 ZmBBX14、 ZmBBX15、 ZmBBX19和 ZmBBX20在玉米叶片中表达量较高， ZmBBX6、 ZmBBX11、 ZmBBX18和 ZmBBX32在玉米叶片和种子形成前期等时期表达量较高。同时，对实验室盐处理转录数据分析发现， ZmBBX1、 ZmBBX3、 ZmBBX9、 ZmBBX12、 ZmBBX13、 ZmBBX21、 ZmBBX25、 ZmBBX29和 ZmBBX33 9个基因在地上和地下都差异表达，其中除 ZmBBX3在叶片和根中表达不同外，其余基因在叶片和根中基本都上调表达。基于全生育期转录组数据的共表达及GO和KEGG富集分析发现， ZmBBX1、 ZmBBX4、 ZmBBX10、 ZmBBX16和 ZmBBX32可能通过蛋白互作介导光依赖的MEbrown模块中萌发籽粒的代谢和生长发育调控。蛋白互作预测分析发现，ZmBBX不仅可以发生同源互作，而且可发生异源互作，并且得到共表达转录数据的支持。综上，ZmBBX基因全生育期组织表达和逆境表达分化明显，在盐等非生物逆境和光相关的生长发育调控中发挥重要作用。 |
| 中文关键词:玉米 BBX 基因家族 表达分析 |
| |
| Identification and Expression Analysis of BBX Gene Family in Maize |
|
|
| Abstract:BBX (B-box) is a family of light-temperature-responsive transcription factors involved in plant photomorpho genes is and stress response. To reveal the ZmBBX function,a bioinformatics method was used to identify the ZmBBX gene at genome level of the maize ,and theirbasic physicochemical properties,gene structure,phylogenetic relationships,cis-acting elements of the promoter,and gene expression profile were analyzed. The maize ZmBBX gene family consists of 34 members,which was divided into five subgroups,its encoding protein amino acids were between 142-498 aa,and most of the members were predicted to be located in the nucleus. The ZmBBX gene promoter had many light response elements,stress response elements,hormone response elements,and growth and development-related cis-acting elements. ZmBBX7, ZmBBX27 and ZmBBX28 were highly expressed at the whole growth stage of maize,while the expression levels of ZmBBX3, ZmBBX5, ZmBBX14, ZmBBX15, ZmBBX19 and ZmBBX20 were high in maize leaves,and the expression levels of ZmBBX6, ZmBBX11, ZmBBX18 and ZmBBX32 increased in maize leaves and early seed formation stage.At the same time, ZmBBX1, ZmBBX3, ZmBBX9, ZmBBX12, ZmBBX13, ZmBBX21, ZmBBX25, ZmBBX29 and ZmBBX33 differentially expressed responses to the salt treatment in aboveground and underground seedlings. All the other genes were up-regulated in seedlings leaves and roots except for ZmBBX3. Further more,based on the co-expression and GO enrichment analysis of transcriptome data during the whole growth period,the module MEbrown which contains ZmBBX gene and has the highest phenotypic correlation was associated with germinated grains,and KEGG results showed that it was related to metabolism. By predictive analysis of protein interactions,it was found that ZmBBX could have not only homologous interactions but also heterologous interactions,which were supported by co-expression transcriptional data. In summary,ZmBBX gene expression and stress expression differentiation are evident throughout the growth period,and it plays a vital role in the regulation of salt and other abiotic stress and light-related growth and development. |
| keywords:Zea mays BBX Gene family Expression analysis |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
|
