Abstract:In this study, family genes of ethylene biosynthesis (MAT, ACS and ACO) pathway in radish (Raphanus sativus L.) were genomewide identified by bioinformatics method and its tissuespecific response to biotic and abiotic stress were analyzed based on transcriptome data and quantitative realtime PCR. The results showed that: (1) a total of 8 MAT, 16 ACS and 7 ACO candidate genes were identified in radish genome and each family could be further divided into three subgroups. (2) The promoter region harbored at least one cisacting element that function in light responsiveness, and cisacting element involved in the phytohormones responsiveness were also found except for ACO1.1 and ACO3. The promoter region of some genes contains cisacting elements involved in response to biotic and abiotic stresses. (3) The analysis of transcriptome data showed that all MAT genes, ACS6.1 and ACO2/3/4 of radish have high expression levels in leaves. Agrobacterium tumefaciens infection significantly upregulated MAT24, ACS2/7.1/7.2 and ACO1.1/1.2/4 in resistance radish inbred lines, while most of them downregulated in susceptible material. The cadmium, chromium and lead stress significantly induced the expression of MAT4.2 and ACO1.1/4, while reduced the expression of MAT1 and ACO5.1. The expression of MAT2.1/2.2/4.1 and ACO1.1/5.2 were significantly suppressed by chilling stress of 4 ℃. (4) qRTPCR results indicated that both NaCl and PEG6000 stress significantly induced the expression of ACO5.1/5.2, but suppressed MAT4.1 and ACO4. Besides, exogenous fructose and sucrose may be involved in the response of radish to PEG6000 stress. Our findings in the present study will be helpful for the functional research of the ethylene biosynthesis genes in radish.