This experiment used ‘Ershuizao’ as the material, detected dynamic changes in the contents of five auxins and their analogs, four cytokinins and abscisic acid in the inflorescence axis (EX), callus (CA), primary embryogenic callus (PC), late embryogenic callus (LC) and globular embryo (GE) stages of garlic by LCMS/MS, combined with the dynamic changes of genes expression related to hormone synthesis and signal transduction. The regulatory effects of the three types of hormones in garlic somatic embryogenesis was discussed. We hope to provide a theoretical basis for the hormone regulation of garlic somatic embryogenesis, and lay a foundation for further research on the molecular mechanism of plant somatic embryogenesis. The results showed: (1) during the garlic somatic embryogenesis, the contents of auxin and its analogs all increased first and decreased later, reaching the highest in the PC stage and the lowest in the GE stage. The contents of N6isopentenyladenine(IP), transzeatin(tZ), and ABA were the highest in the EX stage, and these contents in the other four stages were significantly lower than that in the EX stage, while the content of ciszeatin(cZ) was higher in the CA and PC stages and the lowest in the GE stage. (2) In the PC stage, the expression of the IAA synthesis genes AsYUCCA1, AsTA1, and the extraauxin transport gene AsABCB1 increased. In the LC stage, the expression of auxin transportrelated gene AsAUX1 increased. the expression of auxin response genes AsARF1 and AsARF2 decreased. The expression of the cZ synthesis genes AsLOG1 was the highest in the CA stage. The expression of classifier transportrelated gene AsENT2 and signal transductionrelated genes AsORRA1 and AsORRA2 were the highest in the PC stage. (3) In the last four stages of garlic somatic embryogenesis, the expression of the ABA synthesis ratelimiting enzyme genes AsNCED1 and AsNCED2 were significantly lower than those in the EX stage. The expression of ABA signal transductionrelated genes AsPP2C increased in PC stage, AsABI1 increased in LC stage, and AsPYL1 increased in GE stage. This study suggest that: during garlic somatic embryogenesis, the accumulation of indole3carbaldehyde (ICAld), indole3butyric acid (IBA), and cZ can promote the formation of garlic callus. High levels of auxin and its analogs and low levels of IP and tZ can help the formation of garlic primary embryogenic callus, Low levels of auxin and its analogs, CTK, and ABA may be more conducive to the formation of garlic globular embryo (GE). Hormone synthesisrelated genes regulate the changes in endogenous hormone content, and hormone signal transductionrelated genes were involved in the occurrence of garlic somatic embryos.