Direct live imaging of cell–cell protein transfer by transient outer membrane fusion in Myxococcus xanthus

In bacteria, multicellular behaviors are regulated by cell–cell signaling through the exchange of both diffusible and contact-dependent signals. In a multicellular context, Myxococcus cells can share outer membrane (OM) materials by an unknown mechanism involving the traAB genes and gliding motility. Using live imaging, we show for the first time that transient contacts between two cells are sufficient to transfer OM materials, proteins and lipids, at high efficiency. Transfer was associated with the formation of dynamic OM tubes, strongly suggesting that transfer results from the local fusion of the OMs of two transferring cells. Last, large amounts of OM materials were released in slime trails deposited by gliding cells. Since cells tend to follow trails laid by other cells, slime-driven OM material exchange may be an important stigmergic regulation of Myxococcus social behaviors. DOI: http://dx.doi.org/10.7554/eLife.00868.001


Introduction
Myxococcus xanthus, a gram negative deltaproteobacterium, displays complex multicellular behaviors in response to environmental cues such as the presence of prey bacteria or starvation (Zhang et al., 2012). In particular, starvation triggers a developmental program where thousands of cells coordinate their motility, moving into aggregation centers to build multicellular fruiting bodies where the cells form metabolically-inert spores. This multicellular response requires an arsenal of intercellular signals, including diffusible long-range signals as well as contact-dependent signals (Konovalova et al., 2010;Mauriello et al., 2009). One intriguing cell-cell communication mechanism involves the cell-to-cell transfer of outer membrane (OM) proteins between Myxococcus cells. This phenomenon was originally unmasked by mixing experiments where certain motility mutants were shown to rescue other motility mutants in a process called stimulation (Nudleman et al., 2005). Stimulatable mutants all carried mutations in genes encoding predicted OM proteins (termed cgl or tgl). Experiments with the Tgl and the CglB OM lipoproteins suggested that stimulation is transient and does not involve the exchange of genetic material, but results from the physical transfer of Tgl/Cgl proteins from donor Tgl + /Cgl + cells to recipient Tgl − /Cgl − cells (Nudleman et al., 2005).
Remarkably, OM protein exchange is not restricted to motility proteins and virtually any OM protein and even lipid can be exchanged between cells (Wei et al., 2011;Pathak et al., 2012). Gliding (A−) motility has been shown to be important for transfer, but rather indirectly by promoting the formation of dense regions of aligned cells and favoring intimate cell-cell contacts (Nudleman et al., 2005;Pathak et al., 2012). The transfer process itself depends on two specific proteins, TraA and TraB (Pathak et al., 2012). TraA is a protein with hallmarks of yeast floculins, a class of cell surface adhesins that mediate cell-cell interactions leading to flocculation (Smukalla et al., 2008) and TraB is a secreted protein of unknown function with a possible peptidoglycan-binding domain. TraA and TraB must be expressed both by donor and recipient cells for transfer to occur. Consequently, Wall and colleagues proposed that when adjacent cells engage Tra-dependent surface interactions (i.e., homotypic interactions or interactions with other surface ligands), the OMs fuse locally and OM materials are exchanged (Wei et al., 2011;Pathak et al., 2012). However, because transfer was studied in bulk assays this hypothesis could not be tested directly. Therefore, other mechanisms remained possible, for example long-range exchange of OM vesicles or even local cell lysis. In this study, we investigated the transfer mechanism at the single cell level to gain more insights into the transfer mechanism.

Results
Transfer is a highly efficient OM-specific process In a previous study, Wei et al. (2011) measured the transfer efficiency in agar plate mixing assays ('Materials and methods'), monitoring the appearance of fluorescent recipient cells over time with mCherry fluorescent probes (OM mCherry and IM mCherry ), which when fused to type II or type I signal sequences localize to the OM or the inner membrane (IM), respectively. However, no information was obtained about the increase in fluorescence intensity in the recipient cells. Thus, in a prelude to this study, we repeated the Wei et al. (2011) experiment and further measured fluorescence fluctuations in recipient cells. For completion and to test the transfer of soluble periplasmic proteins, we also constructed a periplasmic probe, fusing mCherry to the Escherichia coli phoA signal sequence (PERI mCherry ) ('Materials and methods' and Figure 1-figure supplement 1). Consistent with previous works and OM specific protein transfer, only OM mCherry was transferred significantly between cells. As observed by Wei et al. (2011), transfer was highly efficient and ∼80% of the total recipient cells were already labeled after 12 hr of co-incubation ( Figure 1A). Transfer remained active for the next 36 hr because although the total number of recipient cells became stable after 24 hr, the fluorescence intensity of eLife digest Bacteria studied in the laboratory are, in general, readily amenable to culture, and they easily form colonies when grown on agar plates. In the wild, however, many bacteria exhibit a range of more complex behaviors, including the growth of super-organisms that contain many cells.
The bacterium Myxococcus xanthus can exist either as single cells or as a super-organism. Each cell has an inner and outer plasma membrane separated by a periplasmic space. Previous work has found that individual cells communicate with each other by exchanging the contents of their outer membranes, and that these swaps can govern multicellular behavior.
Membrane exchange is known to depend on both donor and recipient cells having the proteins TraA and TraB. TraA proteins are similar to the adhesion factors that hold cells together, and they are found in many species: this suggests that TraA therefore might help the outer membranes of cells to fuse so that they can swap materials. The role of TraB is not known at present.
To investigate membrane exchange more closely, Ducret et al. measured the transfer of fluorescent proteins from the periplasm and the inner and outer membranes of the donor cell to the recipient cell, as well as the transfer of fluorescent lipids from the donor's outer membrane. Both proteins and lipids from the outer membrane were transferred rapidly (within minutes); although a small amount of protein transfer from the periplasmic space was observed after 36 hr, there was no transfer from the inner membrane. As in previous studies, exchange depended on the presence of TraA. Ducret et al. observed that contact between two cells was sufficient to stimulate transfer of proteins and lipids from the outer membrane. But not all contacts led to a transfer. Importantly, when cells that had swapped fluorescent membrane components moved apart, they appeared to remain connected by tubular structures, suggesting that an inter-membrane junction must form to allow proteins and lipids to be transferred between the cells. This junction is referred to as an outer-membrane synapse.
Ducret et al. also noted another phenomenon: cells shed pieces of membrane as they moved across surfaces or separated after outer membrane exchange. This suggests that both synapse formation after direct cell-to-cell contact and the shedding of membrane components can help to propagate bacterial signals, enabling population-wide behavioral changes, including the formation or collapse of super-organisms. DOI: 10.7554/eLife.00868.002 recipient cells increased regularly until it reached a plateau at 36 hr ( Figure 1B). After 36 hr of co-incubation, 20% of the recipient cells displayed a high level of fluorescence, showing that some cells acquire exogenous OM content with very high efficiency ( Figure 1C). A low amount of PERI mCherry transfer was detected after 48 hr ( Figure 1A), suggesting that periplasmic proteins may also be exchanged but with a near background level efficiency. These findings confirm results from previous studies that transfer is a highly efficient OM-specific process.
OM transfer can be captured at the single cell level in a live transfer assay We next tested whether OM transfer between two cells could be captured at the single cell level. Although most of the recipient cells are stained after 12 hr, the staining is generally weak and both brilliance and the fast-bleaching of mCherry prevented single cell transfer analysis with the OM mCherry probe. Therefore, to maximize our chances to observe a transfer event, we constructed a new probe where super-folder GFP (sfGFP), a fast folding bright variant of GFP (Pédelacq et al., 2006), is fused to the type II signal sequence (OM sfGFP ). In a bulk transfer assay, OM sfGFP and OM mCherry were transferred with similar efficiencies, showing that OM sfGFP could be used in a single cell assay (Figure 2-figure  supplement 1A). To this aim, Myxococcus donor cells expressing OM sfGFP were mixed with recipient cells expressing IM mCherry, and the emergence of dual color cells was monitored over time by time-lapse fluorescence microscopy. As observed in Figure 2A,B and Figure 2-figure supplement 1D, unlabeled recipient cells became fluorescent when they came in contact with OM sfGFP donor cells (Videos 1,2). Several lines of evidence argue that the observed fluorescence increase results from the physical transfer of OM sfGFP : (i), Fluorescence transfer was very rapid, approximately a third of the total donor fluorescence appeared in the recipient strain after 12 min of contact ( Figure 2-figure supplement  1B). (ii), Fluorescence initially appeared at the contact zone and subsequently diffused throughout the cell body ( Figure 2B). Additionally, green fluorescence was enhanced at the recipient cell periphery, reflecting a membrane localization (Figure 2-figure supplement 1C). (iii), IM mCherry was not exchanged between the two cells ( Figure 2A). (iv), Green fluorescence transfer was not detected in recipient cells that were not in contact with donor cells (Figure 2-figure supplement 1C), or in a negative control experiment, when they were mixed with a traA mutant (Figure 2-figure supplement 1A).
Because transfer seems highly efficient, physical transfer of OM sfGFP would be expected to lead to a decrease in OM sfGFP levels in the donor cells. While indeed a moderate decrease of sfGFP fluorescence is observed in the donor cell ( Figure 2-figure supplement 1B), the steepness of this decrease is likely compensated by the high level of newly synthesized OM sfGFP expressed from the strong pilin (pilA) promoter. To circumvent this limitation, we made use of the observation that lipids are also exchanged during transfer (Pathak et al., 2012) and tested the transfer of DiO, a small C 18 backbone hydrophobic lipid dye that intercalates in lipid membranes. DiO-labelled cells contain a finite amount of DiO and given that it is highly diffusible, its dilution upon transfer should be obvious. Importantly, DiO-transfer is Tra-dependent (Pathak et al., 2012) and thus its exchange between cells would also reflect the transfer dynamics. In a cell mixing experiment, DiO-stained cells were observed to transfer DiO to unlabeled cells upon physical contact ( Figure 2C,D; Video 3). In the example shown in Figure 2C,D, DiO-transfer is also observed to a third cell that is not immediately in contact with the DiO-donor cell but is adjacent to the first transferred cell. Remarkably, the fluorescence of the DiO-labeled cell decreased very rapidly, concomitant with the gradual increase of fluorescence in the adjacent unlabeled cells as if all three cells were connected like communicating vessels ( Figure 2C,D). Transfer must require specific contacts (i.e., collision of TraA proteins) because unlabeled cells do not systematically acquire fluorescence when they establish a direct contact with a DiO-donor ( Figure 2C,D; red contoured cell). In total, the OM sfGFP and the DiO-staining experiments strongly suggest that we were able to capture transfer events at the single cell level. Transfer can occur between more than two cells, potentially explaining why it is facilitated by cell-cell alignment.

Dynamic OM extensions are formed between cells
The DiO experiment suggests that transferring cells are connected like communicating vessels, which would be explained by the formation of transfer sites where the lipid bilayers of each OM fuse locally, giving rise to a single continuous OM between connected cells. What is the evidence for such connections? While imaging OM sfGFP expressing cells or DiO stained cells, we frequently observed tubular structures that appeared when two connected cells moved apart ( Figure 3A, Video 4). These tubes were exclusively derived from the OM because they were only stained by sfGFP when observed in two-color cells expressing both OM sfGFP and IM mCherry ( Figure 3B and

Tube formation is linked to OM transfer
Motile transferring cells may fuse their OMs locally, forming an 'OM synapse'. If such synapses are not resolved when the cells physically separate due to motility, OM tubes would appear because of the tight physical connection. This would predict that tube formation is linked to the transfer mechanism. We first tested whether a tube and the cell OM are continuous. For this, we took advantage of the rapid diffusion of DiO and performed fluorescence recovery after photobleaching (FRAP) experiments targeting a tube connected to a single DiO + cell. DiO fluorescence showed a quick recovery, implying rapid exchange between the tube and the cell DiO pool ( Figure 4A,B). We then aimed to capture tube formation between transferring cells. Since the tubes are relatively short lived, we also used DiO staining for these experiments. In the example shown in Figure 4C and Video 5, DiO is exchanged upon contact between two cells, a tube becomes apparent when the cells move apart, strongly suggesting that tubes are formed between transferring cells. Last, if two cells linked by a tube have continuous OMs, they should exchange DiO, even if they are not immediately in contact. Figure 4D and Video 6, show a DiO-stained tube formed between a brightly fluorescent cell and a weakly fluorescent cell within a larger group of cells. Remarkably, in the cell with weak fluorescence, the level of fluorescence increased steadily as long as the tube connection was maintained, even though the two cells were not in immediate contact ( Figure 4E). When the tube was ruptured the fluorescence decreased due to photo-bleaching ( Figure 4E). Fluorescence transfer was strictly confined to the tube-connected cells and no fluorescent fluctuations were observed in the other cells of the group (Figure 4C,D). Thus, tubes allow the rapid exchange of DiO and must be continuous between two connected cells.
While DiO can be exchanged through the tubes, we did not detect any significant exchange of OM sfGFP or OM mCherry through the tubes (Figure 4figure supplement 1). This is probably not surprising because the tubes are narrow extensions and have a relatively short lifespan (4.2 ± 3 min). Thus, large molecules such as OM sfGFP or OM mCherry with lower diffusion rates than DiO (∼fourfold, Figure 4-figure supplement 2A,B) may traffic slowly through the tubes. OM tubes may allow the transfer of small OM molecules, which may be relevant physiologically but they are likely the manifestation of the intimate contact established between transferring cells. The connection of cells by continuous tubes strongly argue that Myxococcus OM-protein transfer involves the formation of a single OM synapse between two connected cells.

Large amounts of OM materials are deposited in slime trails during single cell motility
Where does transfer occur in the Myxococcus biofilm and why is it highly dependent on motility? Cell alignment in densely packed Myxococcus swarms promotes cell-cell transfer, likely because it favors tight interactions between cells (Nudleman et al., 2005;Wei et al., 2011;Pathak et al., 2012). However, Cryo-EM studies on the Myxococcus biofilm and our TEM and live observations of the lipid tubes also suggests that large amounts of OM materials may be released in the biofilm matrix, which may constitute a significant transfer reservoir (Palsdottir et al., 2009). Interestingly, when we observed gliding cells on cellulose pre-coated EM grids ('Materials and methods'), we found that cells deposit vesicular/tubular material in their wake ( Figure 5A and Figure 5-figure supplement  1). This material was also observed by fluorescence microscopy and must be derived from the OM because dual labeled OM sfGFP /IM mCherry cells deposited trails that were labeled with OM sfGFP but not with IM mCherry (Figure 5B). Gliding Myxococcus cells are known to deposit slime, a self-deposited sugar polymer of unknown composition that facilitates cell adhesion to the underlying substratum . The slime polymer can be detected selectively by addition of fluorescent Concanavalin A (ConA-FITC) in a microfluidic gliding assay . To test whether the OM materials are specifically associated with the deposited slime, we observed slime trails deposited by an OM mCherryexpressing strain in the presence of ConA-FITC. Figure 5B shows that such cells deposited numerous mCherry + dots and tubular structures that co-localized with ConA + trails. EM analysis using goldlabeled ConA confirmed that the deposited OM material is embedded in a sheath of slime polymer ('Materials and methods' and Figure 5C). All together, these results suggest that gliding Myxococcus cells shed a significant amount of their OM during motility and that this material remains attached to the underlying slime polymer. Since gliding Myxococcus cells have long been known to follow trails left by other cells (Burchard, 1982), a tantalizing possibility is that the transfer of OM materials could also occur when cells follow slime trails, harvesting vesicles and tubes embedded in the slime. Unfortunately, we could not test this possibility directly because the amount of OM sfGFP /OM mCherry labeled material remains too weak to detect a significant transfer to gliding cells by fluorescence microscopy.

Discussion
Direct imaging of OM protein transfer between Myxococcus cells uncovers critical aspects of the cell biology and kinetics of transfer. Specifically, we found that the physical contact between two adjacent Research article cells is sufficient to promote transfer of OM proteins and lipids at high efficiency. This explains the results from bulk transfer experiments (from previous works and reported herein) suggesting that transfer is a remarkably efficient process. The formation of transient OM tubes between cells is a major indication that transfer indeed occurs by OM fusion: the tubes are continuous extension of cell OM, they form between transferring cells and allow the rapid exchange of lipids. Importantly, the Myxococcus OM transfer system is distinct from reported bacterial nanotubes, which seem to connect the cytosolic contents of connected cells and involve a yet uncharacterized machinery (Dubey and Ben-Yehuda, 2011). In Myxococcus, the transfer process is restricted to OM proteins and lipids. Transfer only occurs in a subset of cell contact events, suggesting that it is provoked by specific contacts, for example if TraA interactions brought OMs in close apposition locally. OM sfGFP /OM mCherry are fused to type II signal sequences and thus insert in the OM as OM lipoproteins. Since, OM lipoproteins are inserted in the inner leaflet of the OM (Nakayama et al., 2012), transfer must involve the fusion of both leaflets of the OM membrane, suggesting that the entire OM is exchanged locally between cells. The formation of OM synapses must therefore create continuity between the periplasmic content of transferring cells. The size of the OM synapse may be estimated from the size of the tubes (∼50 nm), suggesting that the diameter of the periplasmic lumen may reach up to 20 nm (for an OM of 10-15 nm thickness [Bayer, 1991;Palsdottir et al., 2009]), providing ample space for periplasmic exchange. However, the PERI mCherry probe was poorly if at all exchanged and there is currently no evidence for the physiological transfer of periplasmic proteins, suggesting that OM synapses are not very permeable to periplasmic proteins.
Our results also suggest that gliding motility may facilitate transfer by promoting cell-cell alignment but also when cells follow slime trails by incorporating membrane materials embedded in the slime polymer. The shedding of large amounts of membrane materials on the underlying substrate is a common byproduct of surface motility both in eukaryotic and prokaryotic cells. For example, crawling keratinocytes also deposit their plasma membrane due to the activity of acto-myosin motors in focal adhesions (Kirfel et al., 2003). In Myxococcus, gliding (A−)motility is thought to involve OM dynamics in the form of energized deformations and/or protein movements (Nan et al., 2010;Luciano et al., 2011;Nan et al., 2011;Sun et al., 2011). Thus, OM fragments may detach to the substrate due to the interaction between the motility machinery and slime. It is possible that acquisition of the traAB genes allowed Myxococcus cells to recycle this 'waste' and co-opt it for cell-cell signaling. A tantalizing possibility would be that slime embedded vesicles contain signals that promote specific recognition, facilitate trail following and promote colony expansion in response to environmental changes.
The Myxococcus Tra-dependent cell-cell transfer of OM proteins is a novel mode of bacterial communication that adds to the growing repertoire of bacterial contact-dependent signaling mechanisms. Contrary to known contact dependent protein transfer systems, the type VI secretion (Silverman et al., 2012) or intercellular nanotubes (Dubey and Ben-Yehuda, 2011), the distribution of TraA suggests that Tra-dependent OM fusion is restricted to the deltaproteobacteria ( Figure 6A and Figure 6figure supplement 1). Interestingly, even in Myxococcus xanthus strains, the predicted extracellular N-terminal PA14 domain of TraA shows variability, while the C-terminal region, presumably involved in anchoring to the cell surface is highly conserved ( Figure 6B-figure supplement 2). Importantly, TraA acts as both key and lock for transfer to occur (Pathak et al., 2012). Therefore, as already suggested, OM-transfer may have evolved to regulate interactions between cells of the same kin. tra mutants do not show motility or developmental defects in pure culture and thus the contribution of OM transfer to Myxococcus social behaviors is unclear (Pathak et al., 2012). Interestingly however, mixing tra mutants with WT cells perturbs motility and development profoundly, consistent with a role in the control population dynamics (Pathak et al., 2012). OM exchange by transient fusion may be more widespread than suspected, especially because it is not easily unmasked and likely does not employ a conserved molecular system. Indeed, membrane vesicles and tubes have been observed in other biofilm-forming proteobacteria (Schooling and Beveridge, 2006;Schooling et al., 2009) and could well be involved in OM exchange behaviors.  M. xanthus strains were grown at 32°C in CYE rich media as previously described (Bustamante et al., 2004). When necessary antibiotics were added: kanamycin (Km) at 50 mg/ml, tetracycline (Tc) at 12 mg/ml, for M. xanthus, and Km at 50 mg/ml, or Tc at 100 mg/ml for E. coli. Constructs were confirmed by phenotypes, restriction analysis and DNA sequencing. Plasmids were introduced in M. xanthus by electroporation.

Lipid dye transfer experiment
Cells were first grown in CYE, harvested, resuspended to a final concentration of 1 × 10 7 cfu/ml. To stain cells, 1 µl of Vybrant DiO Cell-Labeling Solution (Invitrogen, Saint Aubin, France) was added to 1 ml of cells and incubated for 30 min in the dark at 32°C under agitation. Cells were then pelleted by centrifugation, and washed four times with 1 ml TPM. Cells were then imaged on agar pads for up to 1 hr.

Time lapse video-microscopy
Time lapse experiments were performed as previously described (Ducret et al., 2009)  inverted epifluorescence microscope TE2000-E-PFS (Nikon, Champigny sur Marne, France). The microscope is equipped with 'The Perfect Focus System' (PFS) that automatically maintains focus so that the point of interest within a specimen is always kept in sharp focus at all times, in spite of any mechanical or thermal perturbations. Photobleaching was performed with a 488 nm laser. The bleach region of interest (ROI) was a circular region ∼1 µm diameter. The ROI was uniformly bleached with a 200 ms laser exposition at 100% intensity. Images were recorded with a CoolSNAP HQ 2 (Roper Scientific, Roper Scientific SARL, France) and a 100x/1.4 DLL objective. All fluorescence images were acquired with appropriate filters with a minimal exposure time to minimize bleaching and phototoxicity effects. Cell tracking was performed automatically using a previously described macro under the METAMORPH software (Molecular devices, Evry, France) (Ducret et al., 2009). Typically, the images were equalized,

Diffusion rates of DiO dye and OM sfGFP fusion
To know the respective diffusions rates of the probes used in this study, we measured the diffusion constant of the DiO and the outer membrane probe OM sfGFP using fluorescence recovery after photobleaching (FRAP). As observed in Figure 4-figure supplement 2A,B, FRAP analysis provided a diffusion coefficient for DiO (D DiO = 8.1 ± 1.3 µm 2 /s) at least four times faster than the OM sfGFP probe (D sfGFP = 2.3 ± 0.5 µm 2 /s). These values are similar to diffusion constants measured for respectively outer membrane probes and outer membrane proteins in E. coli (Tocanne et al., 1994;Chow et al., 2012).

TEM procedure
For TEM experiments carbon-coated copper grids were first coated with carboxymethylcellulose. Briefly, carbon-coated copper grids were covered with 30 µl of carboxymethylcellulose sodium salt (Medium viscosity, Sigma-Aldrich, Inc., St Louis, MO) diluted in ultrapure water. After 15 min of incubation at room temperature, the coating solution was removed by performing two successive washes with ultrapure water. Carbon-coated copper grids were then covered with the cell suspension previously washed and resuspended in TPM containing 100 mM of CaCl 2 (TPM-Ca 2+ ). After 1 or 15 min incubation, unattached cells were removed by performing two successive washes with TPM. For Lectin-Gold staining procedure, carbon-coated copper grids were first covered for 30 min with 30 µl of ConcanavalinA (ConA)-Biotin conjugated (ConA-Biotin; Sigma-Aldrich, Inc.) diluted in TPM-Ca 2+ to a final concentration of 100 µg/ml and then washed four times with TPM-Ca 2+ . The grids were then incubated for 15 min with 10 nm gold-conjugated streptavidin (Invitrogen, Saint Aubin, France) diluted in TPM-Ca 2+ (1/500) and then washed four times with TPM-Ca 2+ . Grids were postfixed with 1% glutaraldehyde, washed once with TPM, washed four times with water, stained with 1% (wt/vol) uranyl acetate, dried, and imaged with a JEM-1011 transmission electron microscope operated at 100 kV. Cells were first observed on standard TEM grids. As observed in Figure 3C, tubes appear as continuous and flexible structures emerging from the cell surface. Unattached tubes and vesicles were also observed in the vicinity of cells suggesting that this material was also released by cells in the media. On standard uncoated TEM grids Myxococcus cells do not glide (data not shown) precluding any observation of these structures when cells were moving. To deal with this limitation we pre-coated the TEM grids with cellulose, a linear polysaccharide composed of β(1→4) linked D-glucose units and previously known to support gliding motility of M. xanthus (Ducret et al., 2013). As observed on Figure 5A,D and Figure 5-figure supplement 1A,B, linear depositions of tubes and vesicles were observed in the wake of motile cells when cells were deposited on pre-coated TEM grids and incubated for 20 min before fixation. Linear depositions were not observed (i) when motile cells were fixed directly after deposition, (ii) with non-motile cells, and (iii) with A− cells (A − S + strain), strongly suggesting that these depositions are specifically associated with the A-motility. Since gliding Myxococcus cells are known to deposit slime, a self-deposited sugar polymer that facilitates cell adhesion to the underlying substratum, we then tested if the deposited material is associated with slime. The slime polymer can be detected selectively by addition of Concanavalin A (ConA). When ConA was added to the TEM grids, electron-dense trails appeared in the wake of motile cells. The tubes and vesicles were clearly embedded in these trails. To verify that the trails are indeed labeled by ConA, we use Biotinylated ConA and colloidal gold-streptavidin. As observed on Figure 5D, gold particles were exclusively associated with the trail proving that vesicles and tubes are associated with the slime polymer.

Periplasmic probe and OM/IM probe Verification-plasmolysis
To verify the proper localization of each probe, cells expressing OM mCherry , IM mCherry , PERI mCherry or OM sfGFP /IM mCherry were subjected to plasmolysis (Lewenza et al., 2008;Wei et al., 2011). As predicted, OM mCherry, OM sfGFP and PERI mCherry retained their envelope localization when IM mCherry probe formed fluorescent cytoplasmic aggregates (Figure 1-figure supplement 1A,B), indicating that following plasmolysis only the IM fusions collapse with the inner membrane. Plasmolysis was performed as previously described (Lewenza et al., 2008). Briefly, log phase cells were washed, resuspended in TPM buffer and then immobilized in a hybrid flow chamber (Ducret et al., 2009). Cells were imaged before (control) and after injection of the plasmolysis solution (0.5 M NaCl).

Lectin staining procedure
Lectin staining was performed as previously described . Briefly, cells were injected in a flow chamber pre-coated with Chitosan. Immediately prior to the experiments, the Concanavalin-A stock solution were diluted to a final concentration of 20 µg/ml in TPM containing 100 mM of CaCl 2 and 100 µg/ml of bovine serum albumin (BSA). The mixture was then injected into the flow chamber. After 20 min of incubation, the lectins were washed out with TPM.