Abstract: Stable isotopes had been widely used in the industrial production, health care, fundamental physics research, and so on. Natural boron has two stable isotopes: ¹⁰B (19.9%) and ¹¹B(80.1%). The thermal neutron cross section of ¹⁰B isotope is 3835 barns, while that of ¹¹B isotope is only 0.005 5 barns. With high cross section for thermal neutron, ¹⁰B isotope gains important applications in nuclear industry, radiation therapy and other fields. In the third-generation nuclear power plants, it’s required that the primary coolant should use enriched boric acid with ¹⁰B isotope in abundance of 30%-60%. Boron carbide with ¹⁰B isotope in abundance of 92% is used in fast neutron reactors as the control rod. ¹⁰B isotope can also be used for boron neutron capture therapy (BNCT) in cancer treatments. In order to obtain high-abundance ¹⁰B isotope, the study on gas centrifugal separation of boron isotopes was carried out based on domestic centrifuges, with boron trichloride as processing gas. Single-centrifuge experiments of different feed flows and cuts were done. Samples of each experiment were analyzed by the gas mass spectrometer to calculate separation performance. As a result, the overall separation factor of boron trichloride was about 1.08. Furthermore, the influence of feed flow and cut on overall separation factor was studied. Finally, based on the single-centrifuge experiments, parameters of centrifugal cascades for high-abundance ¹⁰B isotope production were carried out by numerical calculation. Cascade parameters of square cascade and matched abundance ratio cascade were worked out separately. ¹⁰B isotope production in abundance of 60% and 90% could be obtained by cascades with suitable parameters. The single-centrifuge experimental study and cascade calculation proved the feasibility of boron isotope separation by gas centrifugation method, with boron trichloride as processing gas. It laid a foundation for enriched boron isotopes production.