关键词: 多酚, 超高效液相色谱-串联质谱, 橄榄

Abstract: The aims of the study were to identify and quantify the phenolic compounds present in extracts of Chinese olive fruit (Canarium album L.) and to investigate their respective antioxidant activities. A total of 12 phenolic compounds were identified in Chinese olive fruit by ultra-performance liquid chromatography (UPLC) coupled to time-of-flight mass spectrometry (TOF-MS). A quantitative method was established by UPLC coupled to triple quadrupole mass spectrometry (TQ-MS/MS) under the multiple reaction monitoring (MRM) mode. Phenolic compounds were separated within 15 min. The linearity (r > 0.98), limit of detection (LOQ) (< 1.436 ng/mL), limit of quantification (LOQ) (< 4.786 ng/mL), inter- and intra-day accuracy ( > 80.19%), precision ( > 1.12%) and recovery ( > 80.13%) of the method were all satisfactory and thus it could provide an efficient protocol to analyze phenolic compounds in fruit pulp extracts of Chinese olive. Quantitative analysis of different solvent extracts suggested that 3-O-galloylquinic acid and Geraniin isomers was the most abundant polyphenols in Chinese olive. Furthermore 80% acetone extract contained more polyphenols and possessed stronger antioxidant capacity.

Key words: phenolic compounds, ultra-performance liquid chromatography coupled to triple quadrupole mass spectrometry (UPLC-MS/MS), Chinese olive