食品科学 ›› 2017, Vol. 38 ›› Issue (23): 224-230.doi: 10.7506/spkx1002-6630-201723036

• 营养卫生 • 上一篇    下一篇

乳源生物活性肽QEPV体内外的吸收转运

李婉如1,陈 静1,程志才1,沈 鹏1,高 扬1,李锡安2,张少辉1,3,*   

  1. 1.上海交通大学农业与生物学院,上海 200240;2.浙江熊猫乳业集团股份有限公司,浙江 温州 325800;3.上海交通大学陆伯勋食品安全研究中心,上海 200240
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    浙江辉肽生命健康科技有限公司支持项目

Transepithelial Transport of Milk-Derived Peptide QEPV in Vitro and in Vivo

LI Wanru1, CHEN Jing1, CHENG Zhicai1, SHEN Peng1, GAO Yang1, LI Xi’an2, ZHANG Shaohui1,3,*   

  1. 1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; 2. Zhejiang Panda Dairy Group Co. Ltd., Wenzhou 325800, China; 3. SJTU-Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 发酵乳作为一种长寿食品备受关注。多肽Gln-Glu-Pro-Val(QEPV)是一种来源于发酵乳,具有免疫调节 作用的生物活性肽。通过Caco-2细胞单层膜模型及小鼠模型,研究乳源性生物活性肽QEPV在体外和体内的转运吸 收情况。结果表明:乳源性生物活性肽QEPV具有非常好的稳定性,且能够穿过由Caco-2细胞形成的转运模型,但 当QEPV质量浓度高于3.00 mg/mL时穿膜速率趋于稳定。流式细胞术和超高效液相色谱-四极杆飞行时间质谱结果 表明Caco-2细胞不能吸收QEPV,由于QEPV是不具有空间结构的小肽,可以初步推测出QEPV主要通过胞旁转运方 式透过Caco-2细胞单层膜模型的结论。同时,QEPV可以经过腹腔注射和灌胃方式被小鼠吸收,但肠道吸收效率较 差,腹腔吸收效率较好。

关键词: 生物活性肽, Caco-2细胞单层膜模型, 小鼠模型, 转运吸收

Abstract: Fermented milk benefits human health in many respects. Gln-Glu-Pro-Val (QEPV) is a peptide produced through enzymatic hydrolysis of the milk-derived peptide Gln-Glu-Pro-Val-Leu. QEPV elicits immunomodulatory effects on lymphocytes as a bioactive peptide. In this research, the transepithelial transport of QEPV was investigated in vitro and in vivo by using the Caco-2 cell monolayer model and mouse models. Results showed that QEPV exhibited good stability and it could integrally cross the Caco-2 cell monolayer in vitro. A limited transepithelial transport rate was observed at the concentration of 3.00 mg/mL in the Caco-2 cell monolayer model. Besides, QEPV was not detected in Caco-2 cells by both flow cytometry (FCM) and ultra performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Therefore, QEPV could be absorbed as a bioactive peptide via the paracellular pathway in vitro. The in vivo experiments demonstrated that QEPV could be absorbed by mice via gastrointestinal and peritoneal routes. QEPV was less efficiently absorbed from the intestine than from the peritoneal cavity.

Key words: bioactive peptide, Caco-2 cell monolayer model, mouse model, transepithelial transport

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