粗壮脉纹孢菌降解豆皮粗纤维产可发酵糖培养基优化及单糖分析

doi:10.7506/spkx1002-6630-201523039

食品科学

粗壮脉纹孢菌降解豆皮粗纤维产可发酵糖培养基优化及单糖分析

张玮1，杨建远1,2，范亚苇1,*，邓泽元1

1.南昌大学食品科学与技术国家重点实验室，江西南昌 330047；2.九江学院药学与生命科学学院，江西九江 332000

出版日期:2015-12-15 发布日期:2015-12-24

Optimization of Medium Composition for Soybean Hull Fiber Degradation by Neurospora crassa and Monosaccharide Composition Analysis of the Resulting Fermentable Sugars

ZHANG Wei1, YANG Jianyuan1,2, FAN Yawei1,*, DENG Zeyuan1

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. College of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332000, China

Online:2015-12-15 Published:2015-12-24

摘要/Abstract

摘要：

研究考察了粗壮脉纹孢菌固态发酵豆皮酒糟培养基产纤维素酶的最优培养基组成，并用气相色谱法对该发酵条件下豆皮纤维素降解所产的可发酵糖进行定性检测。结果表明：产纤维素酶的最优培养基组成为豆皮64%、酒糟34%、(NH4)2SO4 2%，在此培养基中粗壮脉纹孢菌发酵产纤维素酶的酶活力为2.83 IU/g，较优化前的1.17 IU/g提高了141.88%，还原糖产量为18.01 g/100 g，较优化前9.91 g/100 g提高了81.74%。获得的可发酵糖的单糖组成主要为葡萄糖和木糖。

关键词: 豆皮, 粗壮脉纹孢菌, 纤维素酶, 可发酵糖, 气相色谱法

Abstract:

In this work, Plackett-Burman design and response surface methodology were applied to optimize the solid
medium containing soybean hull and distillers’ grains to produce cellulase by Neurospora crassa. Meanwhile, the
monosaccharide composition of fermentable sugars from the degraded product under optimal culture medium was analyzed
by gas chromatography. The results showed that the optimal medium was composed of 64% soybean hull, 34% distillers’
grains and 2% ammonia sulfate. Under the optimal condition, the activity of cellulase significantly was improved by
141.88%, from 1.17 IU/g to 2.83 IU/g, and the yield of reducing sugar was increased by 81.74%, from 9.91 g/100 g to
18.01 g/100 g compared to that obtained with the unoptimized medium. The major monosaccharide components of the
resulting fermentable sugars were glucose and xylose, respectively.

Key words: soybean hull, Neurospora crassa, cellulase, fermentable sugar, gas chromatography (GC)

中图分类号:

S216

张玮，杨建远，范亚苇，邓泽元. 粗壮脉纹孢菌降解豆皮粗纤维产可发酵糖培养基优化及单糖分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201523039.

ZHANG Wei, YANG Jianyuan, FAN Yawei, DENG Zeyuan. Optimization of Medium Composition for Soybean Hull Fiber Degradation by Neurospora crassa and Monosaccharide Composition Analysis of the Resulting Fermentable Sugars[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201523039.

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粗壮脉纹孢菌降解豆皮粗纤维产可发酵糖培养基优化及单糖分析

Optimization of Medium Composition for Soybean Hull Fiber Degradation by Neurospora crassa and Monosaccharide Composition Analysis of the Resulting Fermentable Sugars

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