目的 观察丹红注射液对急性血瘀大鼠模型的影响，探讨丹红注射液调控血瘀证血小板功能的作用机制。方法 60只SD大鼠随机分为空白组、模型组、舒血宁注射液（1.8 mL/kg）组和丹红注射液高、中、低剂量（1.4、0.7、0.35 mL/kg）组，尾iv给予药物干预11 d后，采用sc肾上腺素联合冰水浴制备急性血瘀证大鼠模型，再给予药物干预1 d后，检测血流变指标、凝血功能指标，血小板活化黏附、聚集、释放相关指标；观察耳廓微循环、心电图和心尖、腹主动脉组织病理变化；采用qRT-PCR和Western blotting检测心肌组织蛋白激酶B1（protein kinase B1，Akt1）、p38、细胞外信号调节激酶1/2（extracellular signal-regulated kinases1/2，ERK1/2）的表达。结果 与空白组比较，模型组大鼠血液流变学、血管性血友病因子（von Willebrand factor，vWF）、纤连蛋白（fibronectin，FN）、血栓素B₂（thromboxane B₂，TXB₂）、β-血栓环蛋白（β-thromboglobulin，β-TG）、血小板第四因子（platelet factor 4，PF4）指标显著升高（P＜0.05、0.01），6-酮-前列腺素F1α（6-keto-prostaglandin F1α，6-keto-PGF1α）、耳廓微循环血流量显著降低（P＜0.01），心肌组织p-Akt1、p-ERK1/2、p-p38蛋白表达以及Akt1、ERK1/2、p38基因表达水平均显著升高（P＜0.01）。与模型组比较，丹红注射液能够不同程度地降低血液流变学指标、vWF、FN、TXB2、β-TG、PF4（P＜0.05、0.01），升高6-keto-PGF1α（P＜0.05、0.01），显著提高凝血酶时间（thrombin time，TT）值（P＜0.05、0.01），保护心脏，增加耳廓微循环血流量（P＜0.05）。心尖、腹主动脉组织HE染色结果显示，模型组大鼠心肌、血管内膜出现轻微损伤，弹力纤维破坏，由于斑块脱落导致开始出现空泡；丹红注射液组大鼠心肌、血管内膜和内皮细胞以及弹力纤维显著改善。结论 丹红注射液可以恢复血液流变学、凝血功能，改善血管内皮细胞、心肌细胞功能，通过调控vWF/GPIb-IX-V介导的信号通路及血小板功能，对大鼠急性血瘀有较好的保护和治疗作用。

Objective To observe the effect of Danhong Injection (丹红注射液) on acute blood stasis rats model, and explore the mechanism of Danhong Injection on regulating platelet function of blood stasis syndrome. Methods A total of 60 SD rats were randomly divided into blank group, model group, Shuxuening Injection (舒血宁注射液, 1.8 mL/kg) group and Danhong Injection high-, medium-and low-dose (1.4, 0.7, 0.35 mL/kg) groups. After 11d of drug intervention, rat model of acute blood stasis syndrome was prepared with sc adrenaline combined with ice water bath. After 1 d of drug intervention, hemorheology index, coagulation function index, platelet activation, adhesion, aggregation and release related indexes were measured; The auricle microcirculation, ECG and histopathological changes of cardiac apex and abdominal aorta were observed; The expressions of protein kinase B1 (Akt1), p38 and extracellular signal-regulated kinase 1/2 (ERK1/2) in myocardium was detected by qRT-PCR and Western blotting. Results Compared with blank group, hemorheology, von Willebrand factor (vWF), fibronectin (FN), thromboxane B₂ (TXB₂), β-thrombocyclin (β-TG), platelet factor 4 (PF4) were significantly increased in model group (P < 0.05, 0.01), 6-keto-prostaglandin F1α (6-keto-PGF1α) and auricle microcirculation blood flow were significantly decreased (P < 0.01), p-AKT1, p-ERK1/2 and p-p38 protein expressions and Akt1, ERK1/2, and p38 gene expressions in myocardial tissue were significantly increased (P < 0.01). Compared with model group, Danhong Injection decreased the hemorheological indexes, vWF, FN, TXB2, β-TG, PF4 in different degrees (P < 0.05, 0.01), increased 6-keto-PGF1α and thrombin time (TT) (P < 0.05, 0.01), protected heart, increased auricle microcirculation blood flow (P < 0.05). HE staining results of cardiac apex and abdominal aorta showed that myocardial and vascular intima of rats in model group were slightly damaged, elastic fibers were damaged, and vacuoles began to appear due to the plaque shedding; The myocardial, vascular intima, endothelial cells and elastic fibers of rats in Danhong Injection group were significantly improved. Conclusion Danhong Injection can restore hemorheology and coagulation function, improve the function of vascular endothelial cells and cardiac myocytes, and has better protective and therapeutic effects on acute blood stasis in rats by regulating platelet function mediated by vWF/GPIb-IX-V signal pathway.

R285.5

河南省中医药科学研究专项（20-21ZY1001）；河南省高校科技创新团队（23IRTSTHN026）