[关键词]
[摘要]
目的 研究栀子饮片到标准汤剂的量值传递规律。方法 收集23批栀子饮片,制备标准汤剂,测定出膏率,建立饮片和标准汤剂的HPLC指纹图谱,采用三重四级杆飞行时间质谱(triple quadrupole time-of-flight mass spectrometry,Triple-Q-TOF/MS)技术对标定的共有峰进行成分鉴定,对采用对照品比对确认的成分进行含量测定,以出膏率、指纹图谱共有峰传递数及峰面积比值变化、成分转移率为指标,分析栀子饮片到标准汤剂的量值传递规律。结果 栀子饮片指纹图谱标定共有峰26个,其中25个传递到了标准汤剂,共有峰个数传递率96.15%,26个共有峰的成分均得到鉴定,其中7个环烯醚萜类成分栀子苷、京尼平龙胆双糖苷、羟异栀子苷、山栀苷、京尼平苷酸、去乙酰车叶草酸甲酯(DAAME)、鸡矢藤次苷甲酯(SME),2个西红花苷类成分西红花苷I、Ⅱ,2个黄酮类成分芦丁和异槲皮苷,1个单环单萜类成分jasminoside B,1个有机酸类成分绿原酸共计13个成分采用照品比对确认,标准汤剂和饮片共有峰面积比值与成分转移率呈现较好的正向直线关系,7个环烯醚萜类成分合计平均转移率70.90%,其中主要成分栀子苷平均转移率77.16%,西红花苷I、Ⅱ、芦丁、异槲皮苷、jasminoside B、绿原酸平均转移率分别为47.09%、39.56%、63.25%、51.95%、61.42%、75.70%,标准汤剂平均出膏率为28.91%。结论 阐明了栀子标准汤剂量值传递规律,为栀子配方颗粒及含栀子经典名方制剂研究奠定了基础。
[Key word]
[Abstract]
Objective To study the law of quality value transmitting from Zhizi (Gardeniae Fructus, GF) to GF standard decoction. Methods Twenty-three batches of GF were collected and their standard decoction were prepared. HPLC fingerprint of GF and GF standard decoction were established and the components of common peaks in fingerprint were identified by triple quadrupole time-of-flight mass spectrometry (Triple-Q-TOF/MS). The contents of components confirmed by reference substance were determined. Taking the extraction ratio, common peaks transfer number, peak area ratio, and component transfer rate as indexes, the law of quality value transmitting from GF to GF standard decoction was analyzed. Results There were 26 common peaks in the fingerprint of GF, 25 of which were transferred to the standard decoction. and the transfer rate of the common peaks number was 96.15%. The components of 26 common peaks were identified, among them, seven iridoids of geniposide, gentiobioside, gardenoside, shanzhiside, geniposidic acid, deacetyl asperulosidic acid methyl ester (DAAME), and scandoside methyl ester (SME), two crocins of crocin I and crocin Ⅱ, two flavonoids of rutin and isoquercitrin, one monocyclic monoterpenoid of jasminoside B, and one organic acid of chlorogenic acid, a total of 13 components were confirmed by reference substance. The ratio of the common peaks area of GF standard decoction to that of GF showed a good positive linear relationship with the components transfer rate. The average transfer rate of seven iridoids was 70.90%, and that of geniposide was 77.16%. The average transfer rates of crocin I, crocin Ⅱ, rutin, isoquercitrin, jasminoside B, chlorogenic acid were 47.09%, 39.56%, 63.25%, 51.95%, 61.42%, and 75.70%, respectively. The average extraction rate of standard decoction was 28.91 %. Conclusion The value transfer rule of GF standard decoction was clarified, this study laid a foundation for the research of GF dispensing granule formula granules and classic prescription containing GF.
[中图分类号]
R283.6
[基金项目]
江苏省重点研发计划(社会发展)项目(BE2018674);江苏省中医药科技计划项目(YB201836)