目的 评价麦冬醇提物体内外抑制肺癌生长及调控肺癌细胞自噬作用。方法 采用C57BL/6小鼠Lewis肺癌模型,观察麦冬醇提物对肿瘤生长的抑制作用。采用Visual Sonics Vevo 2100小动物专用高频彩色超声仪测量C57BL/6小鼠Lewis肺癌肿瘤的体积,称瘤质量,并计算抑瘤率。采用免疫组织化学法和蛋白免疫印迹法检测肺癌组织中Ki67和P53蛋白的表达。采用电镜观察麦冬醇提物诱导A549肺癌细胞自噬,结合RT-PCR和蛋白免疫印迹法检测诱导自噬相关蛋白表达。结果 麦冬醇提物对Lewis肺癌小鼠的肿瘤生长具有一定的抑制作用,显著降低Ki67蛋白表达,显著增强P53蛋白表达,并且能诱导A549肺癌细胞产生自噬。结论 麦冬醇提物对肺癌具有明显的抑制作用,并能诱导A549肺癌细胞产生自噬,麦冬醇提物的抗肺癌作用可能与其诱导肺癌细胞自噬有关。

Objective To evaluate the effect of Ophiopogon japonicus alcohol extract (OJAE) on tumor growth of lung cancer and the regulation on autophagy of lung cancer cells. Methods Lewis tumor-bearing mice model was established to observe the effect of OJAE on tumor growth. The volume of tumor was observed and calculated by Visual Sonics Vevo 2100 Animal Dedicated High-frequency Color Ultrasound. To detect Ki67 and P53 protein expression in lung cancer tissue by Western blotting assay method and immunohistochemical assay method. The induction of OJAE on autophagy of A549 cells was evaluated by scanning electron microscopy, combining with Western blotting assay and RT-PCR methods. Results OJAE had certain inhibitory effect on Lewis lung cancer tumor growth. It also could make the expression of Ki67 significantly reduced and the expression of P53 significantly enhanced. It could induce autophagy. Conclusion OJAE has obvious inhibitory effect against lung cancer, and has the ability to induce autophagy. This study reveals the anti-lung cancer effect of OJAE may be related to its induction on autophagy, thus provides the basis for the research on anti-lung cancer effect of OJAE.

国家自然科学青年基金项目(81303275);苏州市2012年科技计划医疗器械与新医药专项(ZXY2012009)