The influence of storage time and temperature on propofol concentrations in canine blood and plasma
- Published
- Accepted
- Subject Areas
- Veterinary Medicine, Pharmacology
- Keywords
- Propofol, Stability, Blood, Plasma, Temperature
- Copyright
- © 2017 Cox et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. The influence of storage time and temperature on propofol concentrations in canine blood and plasma. PeerJ Preprints 5:e2885v1 https://doi.org/10.7287/peerj.preprints.2885v1
Abstract
Propofol is an intravenous anesthetic commonly used due to its favorable pharmacokinetic and pharmacodynamic profile. There are discrepancies in the literature about the most appropriate sample for determining propofol concentrations. Although plasma has been used for determining propofol concentrations, whole blood has been the preferred sample because propofol is significantly bound to erythrocytes. There is also a lack of consistency in the literature on the effect of storage time and temperature on propofol concentrations and this may lead to errors in the design of pharmacokinetic/pharmacodynamics studies. The purpose of this study was to determine the difference in propofol concentrations in whole blood versus plasma and to evaluate the influence of storage time (56 days) and temperature (4°C, -20°C, -80°C) on the stability of propofol concentrations in blood and plasma samples. Results from the study indicate that whole blood and plasma samples containing propofol were stable for at least 56 days when stored at -80°C; thus, -80°C is the most appropriate temperature for propofol sample storage out of the three temperatures evaluated. Plasma propofol concentrations were consistently higher than whole blood for all 3 storage temperatures. Consequently, plasma is the most appropriate sample for propofol analysis due to its consistent determinations.
Author Comment
This is a submission to PeerJ for review.