MICROBIAL CONVERSION OF STEFFIMYCIN AND STEFFIMYCIN B TO 10-DIHYDROSTEFFIMYCIN AND 10-DIHYDROSTEFFIMYCIN B
1980 Volume 33 Issue 8 Pages 819-823
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1980 Volume 33 Issue 8 Pages 819-823
It has been shown that steffimycin (1) and steffimycin B (2) are reduced at the C-10 carbonyl by Actinoplanes utahensis, UCR-5885 and Chaetomium sp., UCR-4634, respectively. Using cell-free extracts of the latter organism, the optimum conversion time, pH, and enzyme concentration have been determined for the conversion of 2 to 4. The biochemical conversion of 2 has been found to be TPNH linked.
