本文描述一種從臺灣植物分離而得之新穎性酵母菌。該分離株9506015(上標 T)分離自新北市深坑區的蕨類植物,不產生有性孢子與投擲孢子。經由26S核糖體核糖核酸基因序列分析顯示,與本種親緣相近之酵母菌種分別為Cryptococcus zeae、Bullera kunmingensi、C. luteolus、B. sinensis,其序列相似度只有96.0%-96.7%。以內轉錄間隔區(ITS)之序列分析,相似度低至91.2%-96.2%。除此之外,本新種亦能以生理特徵方式,與其他親緣相近之酵母菌種區分,如肌酸、肌酸酐與咪唑等氮源之利用,於0.1%的環己醯亞胺中生長。綜合形態、生理及基因序列數據,提議該菌種為Cryptococcus屬之新種Cryptococcus taiwanensis。該新種之標準菌株為BCRC 23252(上標 T) (=CBS 12180(上標 T))。
During a continuous survey of yeast diversity in Taiwan, an anamorphic yeast, belonging to the genus Cryptococcus was obtained. To determine the species identity, this yeast was analysed morphologically, physiologically, and phylogenetically. Neither ballistoconidia nor sexual reproduction was observed. Sequence analysis of 26S rRNA gene indicates that Cryptococcus zeae, Bullera kunmingensi, Cryptococcus luteolus, Bullera sinensis are the most closely related species, with 96.0 to 96.7% sequence similarity. But they only show low sequence similarity with the yeast obtained in this study in the ITS region (91.2 to 96.2%), and differ physiologically from it in the ability to assimilate creatine, creatinine and imidazole, and to grow in 0.1% cycloheximide. Judging from these comparative analyses, a novel species, Cryptococcus taiwanensis, is proposed, with BCRC 23252(superscript T) (=CBS 12180(superscript T)) as the type strain.