Endophytic fungi associated with the leaves of Bambusa tuldoides were investigated. Quantitative and qualitative differences were found between the endophyte assemblages from different ages of leaves collected from the upper and lower culm levels. More isolates were recovered from older than from younger leaves, sampled from both the upper and lower culm levels. Leaf tissues with secondary veins supported more endophytes, compared to those with primary veins. There were no significant differences in the communities distributed throughout the leaves.