本研究分別以實驗及理論之方法,探討流體流經Sepharose軟粒子床之流力行為。從粒子強度與粒子間接觸模式之關係,以及在粒子床阻力的估量上,針對因流體流動所形成之摩擦拖曳力以及粒子本身重量,所造成之粒子壓縮而導致孔隙度減少之效應等方面,皆加以探討。本研究之理論分析結果,與Colby等(1996)所得之實驗數據進行比較,可的知此理論分析方式,能夠準確地預測在不同流體流動速率下,軟粒子填充床所造成之穩態壓降變化。
The hydrodynamic behaviors of fluid flow through particles bed composed of soft Sepharose gel particles were studied theoretically and experimentally. A number of in situ strengths of single gel particle and inter-particle contact modes among gel particles were explored. Effect of particle deformation due to frictional drag and mass of particles on the reduction of porosity was studied to examine how this variation led to the increase in flow resistance. The expansion of gel particles bed after the release of hydraulic drag force was observed. Theoretical predictions were compared to experimental pressure-drop data obtained by Colby et al. (1996). Results showed that the proposed theory could provide accurate steady-state pressure-drop predictions in gel particles bed composed of soft gel particles at different superficial velocities.