Medical device that contacts with human body has to evaluate safety of in vitro cytotoxicity . In recent years, nanomaterials are used widely in the field of medical devices. In this study, we used nanomaterials and non-nanomaterials to establish an in vitro cytotoxicity test platform which was according to the relevant standards of the International Organization for Standardizations (ISO) and The American Society for Testing and Materials (ASTM). We selected the listed product “High Efficiency Leukocyte Removal Filter For Red Cell Transfusion (RCXL1Y)” and “nanosilver particle” to test the above platform. The result of RCXL1Y cytotoxicity test was non-cytotoxic. However, as the result of nanosilver, it appeared interference phenomenon that led to false result. We found that the test methods of standards were not suitable for all nanomaterials. Therefore, using test methods of standards should notice interference conditions to ensure the correction of results. In the future, this study about in vitro cytotoxicity testing platform can be used in developing medical devices to promote progress in the industry.